LOCUS       40924_7591        5904 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Broad host range vector pBTBXh-6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Broad host range vectors for expression in Gram negative hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2009) Chemical and Biological Engineering, 
            University of Colorado, UCB 424, Boulder, CO 80309, USA
REFERENCE   3  (bases 1 to 5904)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5904)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2009) Chemical and Biological Engineering, University of 
            Colorado, UCB 424, Boulder, CO 80309, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..21
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      complement(74..110)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      116..885
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             886..1545
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1645..2418
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="involved in tellurite resistance"
                     /protein_id="ACV83917.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            1645..2418
                     /gene="kilA"
                     /label=kilA
     CDS             2436..3572
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /protein_id="ACV83918.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            2436..3572
                     /gene="telA"
                     /label=telA
     CDS             3569..4522
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /protein_id="ACV83919.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            3569..4522
                     /gene="telB"
                     /label=telB
     terminator      complement(4536..4567)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(4598..5473)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5500..5784
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 gaccatcatc atcatcatca ttagagcttg atatcattca ggacgagcct cagactccag
       61 cgtaactgga ctgaaaacaa actaaagcgc ccttgtggcg ctttagtttt gttccgcggc
      121 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag
      181 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc
      241 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt
      301 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa
      361 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg
      421 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc
      481 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc
      541 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc
      601 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga
      661 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca
      721 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga
      781 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc
      841 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc
      901 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa
      961 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat
     1021 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc
     1081 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag
     1141 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat
     1201 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc
     1261 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc
     1321 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc
     1381 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa
     1441 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat
     1501 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagta cgtaagaggt
     1561 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1621 ttttcaggag ctaaggaagc taaaatggaa gaacaaagcg tgaacatggc gcgattgaag
     1681 ggggaggttt tgcccgccct cttcgcgtcg ccggcgacga ttggcgagta cggggccggc
     1741 atcgacgggg cggattccct caacgagctg tcgaatctga tggagcacgg cgcagttgcc
     1801 gcgctggccg acaaaatcag ccagatcgtg gcgaagctgg ccgacgcgga cccccgcaag
     1861 atcgcggaaa agcctacctg gttcgagaag atgcttggcc gtgaggttga acgccaggtg
     1921 aggtatcagg tcgcccgcaa gacgctcgac cagttgctgg acgaagccga gggcgtagcg
     1981 cagcgcgtgc gggacacgtt gcgcgccttg gatgacatgc tcaatacgca tgaggccgag
     2041 gtagaccggc tcagagccta cattcaagcc gggcgcgagt tcctggacga gaaccccgag
     2101 gccggcgcgg ccaaggccgg cgtgatcgag ttcgacaagc cgcgcgaacg cttcgcgcgc
     2161 aagctcgcca acctggcaac cctcatggcg tcccatgaaa tgagcgtcac tcagatgaag
     2221 ctcacgcggg cgcaggccgt ggacatgctg gaccgcttct ctgaaacggc atccgtcctg
     2281 gtgcccgtct ggcgtcagca caccctcgcg ctcatcacca ccaagaacat gaatccggca
     2341 atggtcgccg aggcggccaa agctcaccag gcgctcatgc ggagcctttc gcagagcctg
     2401 gaaggcatca accaataaca cggcgggaga accctatgaa cgcactgaaa acgacgcacg
     2461 acgccaaggc ccctatcgtc gccttcgaca tgaccccggc aaccctgcgc gagctgggct
     2521 tgcaggaaag cgacgtgccg gaagtccatg cggtcgcgca gcggatcgag gtcggcagtc
     2581 cgcagaccgt tgccgagttc ggccgcgacg tggccgagca cacgtcccgc tacgccgata
     2641 gcctgctgga ccaggtgcgc aacagcgacc tggacgaagc aggcgagaaa ctgacccagg
     2701 ttgtcgccaa ggcccgttcc ctgaacgtcg gccctttgtc cgacaaccgt tcccgcctgc
     2761 ccctgattgg cccgctgatc gaccgcttcc gcgtccgttc gacgggcttc atggcgcgct
     2821 tcgacacgac ccgcgagcag atcgaacacc tggtcagcga agtgcagacc acccagcaag
     2881 gcatcgcgca gcgcaatgcc tcgctcgacg aaatgttcgc agccgtgcgc gaggaacacc
     2941 gccttcttgg cgtccacatc gcggccggca aggtccgcct tgccgagctg cgcgagcagg
     3001 ccgagggtct gcgcggcaat gtcgggaacg acccgggccg cgtgcaggag ctggccgacc
     3061 tcgatgcgat ggttgccaac ctggacaagc gcatcggcga cctgatcgcc ttgcaacatt
     3121 cggccatgca gagcctgccg accatccgca tgatccaggc caacaaccag atgctggtcg
     3181 ataaattcca caccatccgc gaaatcaccg tgccggcgtg gaagcggcaa ttcatgctgg
     3241 ccttgagcct caacgagcag aagaacgccg tcgaactggc cacggccatc gacgacacca
     3301 ccaacgacct gatgaagcgc aatgcggccc tgctgcatcg cacgtccgtc gagacggcga
     3361 aggagaacca acgcctggtg atcgacgtgg acacgctcaa gcaggttcag acgacgctca
     3421 tcaagaccgt cgaggacgtt attcgcatcc agcaggaagg cgtgcagaag cgcaaggatg
     3481 ccgagaagca gatcgccgca atgcgtggcg atcttcaagc caagctgacc cgccagcccg
     3541 tgcgcgagct ggcccaacag gagtccgtat gaatgccaca aacaccgatg ttttcgccca
     3601 ggtaggcggc ctcgaggccc gaggcgcgaa gatgaagaag cggggcaccc gcttcctcat
     3661 cgcggcgctg gcagtccttg ccattgccgg gatcggggca gtaacgggat gggcgatcag
     3721 cccgagcgcg acgcccggaa gcattgacgt gccgcaggtg ctggcatcga cattcagcga
     3781 ccaggtgccg ggcagtgagg gcggcggcct gggtggcggc ctgcccttca cttcggccgt
     3841 cggggcattc acggacttca tggcggggcc ggcaattttt accttgggca ttcttggcat
     3901 agtggtcgcg ggtgccgtgc tcgtgttcgg gggtgaattc tgcgggttcg tgcgatccgt
     3961 ctgcatgatg gtgatagccg tcagcatgat tttcgtgtcg tcgaacttgg tgaagggcat
     4021 tctcggcggc gatcacgacg ccggccctgc ggagccttcg ccgcgtgcgc gattcatggc
     4081 ggccgtggag gccaaggatt tcgcgcgagt gcaagagctg atcgaggcgc gtggagccaa
     4141 gtcggcggct gattatgtcc ttgcgcagct cgccgtggcc gaaggtctgg accgcaagcc
     4201 tggtgcgcgc gtcgtggtcg ggaaagcggc gggcagcatg gcaatgccgc ctgcggcgct
     4261 gggttttacg ccaaggggag aagcggcata cgccatcgag cggtcagcct atggtgagcc
     4321 gaggtccagc attgcgaagc agtaccagca ggaatggaac cggaaggcgg cgacctggtg
     4381 ggcgatggcc ggtgtggccg gcatcatcgg cgcgatcctg gcggcggcgg caaccggctt
     4441 tgttgggctg gcagtgtcga tccgcaaccg agtgaagcgc gtgcgcgacc tgttggtgat
     4501 ggagccgggt gcagagccat aagggatccc cctcaagtca aaagcctccg gtcggaggct
     4561 tttgactttc tgctatggag gtcaggtatg atttttatga caacttgacg gctacatcat
     4621 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
     4681 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     4741 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     4801 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     4861 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     4921 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     4981 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     5041 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     5101 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     5161 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     5221 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     5281 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     5341 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     5401 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     5461 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
     5521 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
     5581 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     5641 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     5701 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
     5761 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaccgcc tatctcgtgt
     5821 gagataggcg gagatacgaa ctttaagaag gagatatacc catggtaaaa tggtcagtat
     5881 tgagcgatat ctagagaatt cgtc
//
