LOCUS       40924_7586        3266 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Broad host range vector pBTBXh-5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Broad host range vectors for expression in Gram negative hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2009) Chemical and Biological Engineering, 
            University of Colorado, UCB 424, Boulder, CO 80309, USA
REFERENCE   3  (bases 1 to 3266)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2009) Chemical and Biological Engineering, University of 
            Colorado, UCB 424, Boulder, CO 80309, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..21
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      complement(74..110)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      116..885
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             886..1545
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1645..1878
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     terminator      complement(1898..1929)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(1960..2835)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2862..3146
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 gaccatcatc atcatcatca ttagagcttg atatcattca ggacgagcct cagactccag
       61 cgtaactgga ctgaaaacaa actaaagcgc ccttgtggcg ctttagtttt gttccgcggc
      121 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag
      181 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc
      241 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt
      301 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa
      361 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg
      421 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc
      481 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc
      541 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc
      601 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga
      661 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca
      721 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga
      781 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc
      841 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc
      901 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa
      961 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat
     1021 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc
     1081 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag
     1141 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat
     1201 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc
     1261 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc
     1321 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc
     1381 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa
     1441 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat
     1501 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagta cgtaagaggt
     1561 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1621 ttttcaggag ctaaggaagc taaaatgggt caaagtagcg atgaagccaa cgctcccgtt
     1681 gcagggcagt ttgcgcttcc cctgagtgcc acctttggct taggggatcg cgtacgcaag
     1741 aaatctggtg ccgcttggca gggtcaagtc gtcggttggt attgcacaaa actcactcct
     1801 gaaggctatg cggtcgagtc cgaatcccac ccaggctcag tgcaaattta tcctgtggct
     1861 gcacttgaac gtgtggccta agaatggatc cccctcaagt caaaagcctc cggtcggagg
     1921 cttttgactt tctgctatgg aggtcaggta tgatttttat gacaacttga cggctacatc
     1981 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
     2041 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
     2101 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
     2161 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
     2221 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
     2281 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
     2341 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
     2401 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
     2461 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
     2521 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     2581 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     2641 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     2701 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     2761 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     2821 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     2881 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     2941 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     3001 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     3061 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     3121 atcgcaactc tctactgttt ctccataccc gtttttttgg gctagcaccg cctatctcgt
     3181 gtgagatagg cggagatacg aactttaaga aggagatata cccatggtaa aatggtcagt
     3241 attgagcgat atctagagaa ttcgtc
//
