LOCUS       40924_7561        5890 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Broad host range vector pBTBX-6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Broad host range vectors for expression in Gram negative hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2009) Chemical and Biological Engineering, 
            University of Colorado, UCB 424, Boulder, CO 80309, USA
REFERENCE   3  (bases 1 to 5890)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2009) Chemical and Biological Engineering, University of 
            Colorado, UCB 424, Boulder, CO 80309, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..871
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             872..1531
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1631..2404
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="related to tellurite resistance"
                     /protein_id="ACV83871.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            1631..2404
                     /gene="kilA"
                     /label=kilA
     CDS             2422..3558
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="related to tellurite resistance"
                     /protein_id="ACV83872.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            2422..3558
                     /gene="telA"
                     /label=telA
     CDS             3555..4508
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="related to tellurite resistance"
                     /protein_id="ACV83873.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            3555..4508
                     /gene="telB"
                     /label=telB
     terminator      complement(4522..4553)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(4584..5459)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5486..5770
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atggaagaac aaagcgtgaa catggcgcga ttgaaggggg aggttttgcc
     1681 cgccctcttc gcgtcgccgg cgacgattgg cgagtacggg gccggcatcg acggggcgga
     1741 ttccctcaac gagctgtcga atctgatgga gcacggcgca gttgccgcgc tggccgacaa
     1801 aatcagccag atcgtggcga agctggccga cgcggacccc cgcaagatcg cggaaaagcc
     1861 tacctggttc gagaagatgc ttggccgtga ggttgaacgc caggtgaggt atcaggtcgc
     1921 ccgcaagacg ctcgaccagt tgctggacga agccgagggc gtagcgcagc gcgtgcggga
     1981 cacgttgcgc gccttggatg acatgctcaa tacgcatgag gccgaggtag accggctcag
     2041 agcctacatt caagccgggc gcgagttcct ggacgagaac cccgaggccg gcgcggccaa
     2101 ggccggcgtg atcgagttcg acaagccgcg cgaacgcttc gcgcgcaagc tcgccaacct
     2161 ggcaaccctc atggcgtccc atgaaatgag cgtcactcag atgaagctca cgcgggcgca
     2221 ggccgtggac atgctggacc gcttctctga aacggcatcc gtcctggtgc ccgtctggcg
     2281 tcagcacacc ctcgcgctca tcaccaccaa gaacatgaat ccggcaatgg tcgccgaggc
     2341 ggccaaagct caccaggcgc tcatgcggag cctttcgcag agcctggaag gcatcaacca
     2401 ataacacggc gggagaaccc tatgaacgca ctgaaaacga cgcacgacgc caaggcccct
     2461 atcgtcgcct tcgacatgac cccggcaacc ctgcgcgagc tgggcttgca ggaaagcgac
     2521 gtgccggaag tccatgcggt cgcgcagcgg atcgaggtcg gcagtccgca gaccgttgcc
     2581 gagttcggcc gcgacgtggc cgagcacacg tcccgctacg ccgatagcct gctggaccag
     2641 gtgcgcaaca gcgacctgga cgaagcaggc gagaaactga cccaggttgt cgccaaggcc
     2701 cgttccctga acgtcggccc tttgtccgac aaccgttccc gcctgcccct gattggcccg
     2761 ctgatcgacc gcttccgcgt ccgttcgacg ggcttcatgg cgcgcttcga cacgacccgc
     2821 gagcagatcg aacacctggt cagcgaagtg cagaccaccc agcaaggcat cgcgcagcgc
     2881 aatgcctcgc tcgacgaaat gttcgcagcc gtgcgcgagg aacaccgcct tcttggcgtc
     2941 cacatcgcgg ccggcaaggt ccgccttgcc gagctgcgcg agcaggccga gggtctgcgc
     3001 ggcaatgtcg ggaacgaccc gggccgcgtg caggagctgg ccgacctcga tgcgatggtt
     3061 gccaacctgg acaagcgcat cggcgacctg atcgccttgc aacattcggc catgcagagc
     3121 ctgccgacca tccgcatgat ccaggccaac aaccagatgc tggtcgataa attccacacc
     3181 atccgcgaaa tcaccgtgcc ggcgtggaag cggcaattca tgctggcctt gagcctcaac
     3241 gagcagaaga acgccgtcga actggccacg gccatcgacg acaccaccaa cgacctgatg
     3301 aagcgcaatg cggccctgct gcatcgcacg tccgtcgaga cggcgaagga gaaccaacgc
     3361 ctggtgatcg acgtggacac gctcaagcag gttcagacga cgctcatcaa gaccgtcgag
     3421 gacgttattc gcatccagca ggaaggcgtg cagaagcgca aggatgccga gaagcagatc
     3481 gccgcaatgc gtggcgatct tcaagccaag ctgacccgcc agcccgtgcg cgagctggcc
     3541 caacaggagt ccgtatgaat gccacaaaca ccgatgtttt cgcccaggta ggcggcctcg
     3601 aggcccgagg cgcgaagatg aagaagcggg gcacccgctt cctcatcgcg gcgctggcag
     3661 tccttgccat tgccgggatc ggggcagtaa cgggatgggc gatcagcccg agcgcgacgc
     3721 ccggaagcat tgacgtgccg caggtgctgg catcgacatt cagcgaccag gtgccgggca
     3781 gtgagggcgg cggcctgggt ggcggcctgc ccttcacttc ggccgtcggg gcattcacgg
     3841 acttcatggc ggggccggca atttttacct tgggcattct tggcatagtg gtcgcgggtg
     3901 ccgtgctcgt gttcgggggt gaattctgcg ggttcgtgcg atccgtctgc atgatggtga
     3961 tagccgtcag catgattttc gtgtcgtcga acttggtgaa gggcattctc ggcggcgatc
     4021 acgacgccgg ccctgcggag ccttcgccgc gtgcgcgatt catggcggcc gtggaggcca
     4081 aggatttcgc gcgagtgcaa gagctgatcg aggcgcgtgg agccaagtcg gcggctgatt
     4141 atgtccttgc gcagctcgcc gtggccgaag gtctggaccg caagcctggt gcgcgcgtcg
     4201 tggtcgggaa agcggcgggc agcatggcaa tgccgcctgc ggcgctgggt tttacgccaa
     4261 ggggagaagc ggcatacgcc atcgagcggt cagcctatgg tgagccgagg tccagcattg
     4321 cgaagcagta ccagcaggaa tggaaccgga aggcggcgac ctggtgggcg atggccggtg
     4381 tggccggcat catcggcgcg atcctggcgg cggcggcaac cggctttgtt gggctggcag
     4441 tgtcgatccg caaccgagtg aagcgcgtgc gcgacctgtt ggtgatggag ccgggtgcag
     4501 agccataagg gatccccctc aagtcaaaag cctccggtcg gaggcttttg actttctgct
     4561 atggaggtca ggtatgattt ttatgacaac ttgacggcta catcattcac tttttcttca
     4621 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa
     4681 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
     4741 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
     4801 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg
     4861 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
     4921 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
     4981 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
     5041 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga
     5101 aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc
     5161 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga
     5221 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc
     5281 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
     5341 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
     5401 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
     5461 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
     5521 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa
     5581 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
     5641 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
     5701 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
     5761 gtttctccat acccgttttt ttgggctagc accgcctatc tcgtgtgaga taggcggaga
     5821 tacgaacttt aagaaggaga tatacccatg gtaaaatggt cagtattgag cgatatctag
     5881 agaattcgtc
//
