LOCUS       40924_6606        7441 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBK638, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Schoep TD, Gregg K.
  TITLE     Primer Extension Analysis of Gene Promoters by Fluorescent DNA 
            Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Schoep TD, Gregg K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2005) Microbiology and Immunology, University of 
            Western Australia, Stirling Hwy, Perth, WA 6009, Australia
REFERENCE   3  (bases 1 to 7441)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7441)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2005) Microbiology and Immunology, University of Western 
            Australia, Stirling Hwy, Perth, WA 6009, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..338
                     /note="bacteriophage T4D gene 32 transcription/translation 
                     terminator"
                     /regulatory_class="terminator"
     regulatory      339..875
                     /regulatory_class="promoter"
     regulatory      568..573
                     /regulatory_class="minus_35_signal"
     regulatory      591..596
                     /regulatory_class="minus_10_signal"
     regulatory      680..685
                     /regulatory_class="minus_35_signal"
     regulatory      703..708
                     /regulatory_class="minus_10_signal"
     CDS             897..1520
                     /codon_start=1
                     /gene="ermAM"
                     /product="erythromycin resistance protein"
                     /label=ermAM
                     /protein_id="ABD13962.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRCTLDIHRTLG
                     LLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNRE
                     YRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            897..1520
                     /gene="ermAM"
                     /label=ermAM
     CDS             2067..2867
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3013..3601
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3889..3910
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    3965..3981
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3989..4005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4043..4061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4068..4084)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4792..4808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5278..6132)
                     /codon_start=1
                     /product="ORF2"
                     /label=ORF2
                     /protein_id="ABD13964.1"
                     /translation="MSHNMILLFSEGITMKNDDRMEIAIERDYKVVKANEIIQKAKFDL
                     GLLEQKTFCYAVSKIKPDDALGSEYVFTINEYCDICGINRNSGKTIEEVKSALKRLRDK
                     SFYMLDEKGDYVLIGWLSKAKVSPRSGKIAIKFDEDMQKYLMGLYTNYTQYSLLCVLPM
                     RSTYSIRLYELLKSYAGLHKKDFDIDDLKAQLCAPYARFPDFRRKVIEVAVKEINIYTD
                     IEVSWEPINKGRKVVKVHFSIKQRNSMKIAMAGARATEALDGIDGQIRFDLEGNLSEER
                     KSR"
     CDS             complement(6393..6830)
                     /codon_start=1
                     /product="putative replication protein"
                     /label=putative replication protein
                     /note="ORF1"
                     /protein_id="ABD13965.1"
                     /translation="MTITEFAESRQVQPQAISRYIGRHPEKFNGHTEKKGKTVELDDIA
                     LELLEKKYPMPAPVQIIEDTESRQKLIKAQELIIQLQDKLMDAQSQIAEAEATKILLED
                     KNAQIEKYELTEANYKKQIDELLEELSKEKSKTWIDKLFKK"
ORIGIN
        1 aagcttgcat gcctgcaggt cgatcgactc tagcggatca gtgagggttt gcaactgcgg
       61 gtcaaggatc tggatttcga tcacggcacg atcatcgtgc gggagggcaa gggctccaag
      121 gatcgggcct tgatgttacc cgagagcttg gcacccagcc tgcgcgagca ggggaattga
      181 tccggtggat gaccttttga atgaccttta atagattata ttactaatta attggggacc
      241 ctagaggtcc ccttttttat tttaaaaatt ttttcacaaa acggtttaca agcataaagc
      301 ttgctcaatc aatcaccgga tcgtcgacag gcctcgagag gggttaggga tgtaaatcgt
      361 ttattgggat ttctggcagg attaatagaa ggaataataa taatatggct tatcctcttc
      421 attgcagact gcttcccggc ctctgcgtat ggcagattta tcatagaaaa cacagaaaat
      481 gaccagatgt tgtattttat ttatcaaaac aacataattg agagaattat cggaatataa
      541 agacattaat tattttgaaa aaacctgttg actttgtggt agtgggatga taatatacta
      601 ttcgttccgc gagagcagaa cacaagatga aatcgagtgt ttcatcaaaa agattaaaaa
      661 aataaatctt aaaaagtgct tgacaaagca agctgctcat gataatataa acgagttgct
      721 gctgagaagc acaacaaacg aagattgata actgaacagt gaaacaaacc ttgaattaat
      781 acaagttttt aaaacatgta tccttgaaat tcatttaagt ttcttaatga aacgaaacct
      841 ttattagtaa acaagtcagt tttatactga ttcggcatat gaagaggaga gatcccatga
      901 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
      961 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
     1021 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
     1081 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
     1141 aagatattct acagtttcaa ttccctaaca aacagaggtg taccttggat attcaccgaa
     1201 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
     1261 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
     1321 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
     1381 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
     1441 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
     1501 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
     1561 actaaaggga atgtagataa attattaggt atactactga cagcttccaa ggagctaaag
     1621 aggtccctag cgcctacggg gaatttgtat cgataagggg tacaaattcc cactaagcgc
     1681 tcggggggta ccgcgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     1741 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1801 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1861 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     1921 ttataggtta atgtcatggg ggggggcacg ttgtgtctca aaatctctga tgttacattg
     1981 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2041 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgacgagg ccgcgattaa
     2101 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     2161 caggtgcgac aatctaccga ttgtatggga agcccgatgc gccagagttg tttctgaaac
     2221 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     2281 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     2341 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
     2401 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     2461 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     2521 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     2581 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     2641 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     2701 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2761 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2821 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2881 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     2941 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3121 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     3421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3781 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3841 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     3901 gctcactcat taggcacccc aggctttaca ctttatgctt ccgcggctcg tatgttgtgt
     3961 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccact
     4021 agtggatcga tccccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     4081 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4141 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4201 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4261 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4441 ctctaaatcg gggtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4501 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4561 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4621 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgaggctg cgcaactgtt
     4681 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     4741 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     4801 cggccagtgc caagcttacc gctcccccag gcgaagccta accctcttgc tacaagggtc
     4861 tatcgaccct tacgaaaaaa cgaactaaca ctttgctatc tgaacaaagt gctagttcgt
     4921 tttttctatc cctgaaaccc tttttattct tttaaagaac cacacaattt ccaaaatgca
     4981 attttattac taacattctt ggcaagcagg gcaaatggat atccatttgc ttttttttgc
     5041 aaaaaacttg ttggggaata tccccaatcc ccaatcaaaa ttaaaatttt gagcaacgct
     5101 cttcgagcta ctttttttaa aatcttccgt aaatcaaaaa tgatttatta attatatcca
     5161 gcaagctgga agagtttgta agcggctaaa aagattcgaa cttcactagg caacaaaata
     5221 cgtttgccaa gtgaattcga ctttttaacc tacagcccac aaacttataa aattggatca
     5281 gcgacttttt ctttcttcag acaaattacc ttccagatca aatctaattt gtccatctat
     5341 tccgtccaag gcttctgttg ctcttgcacc agccattgca attttcattg agtttctctg
     5401 ctttatgcta aaatgcacct ttaccacttt gcgtcctttg ttaattggct cccatgacac
     5461 ttctatgtct gtatatatat taatttcttt tactgccact tcaataacct tccttctaaa
     5521 atcaggaaat cttgcataag gagcgcacag ttgagctttc aaatcatcta tatcaaaatc
     5581 ttttttgtga agcccagcat agctcttcaa aagttcatag agccttatag aatatgtcga
     5641 gcgcattggt agaacacaaa gcaagctata ctgggtgtaa tttgtataaa gtcccatcaa
     5701 atacttttgc atatcttcat caaacttgat tgctattttg ccactcctag gactgacttt
     5761 tgcttttgaa agccaaccaa tcagaacata atctcctttt tcatcaagca tataaaaaga
     5821 cttatcacgt agtcttttta gtgcactttt aacttcttca attgttttac cactatttcg
     5881 attgattcca cagatatcgc agtattcgtt tattgtaaaa acatactcgc tgcccaaagc
     5941 gtcatcaggc tttatttttg aaaccgcgta gcagaaagtt ttttgctcta acaatcctaa
     6001 atcaaacttt gctttttgaa taatctcatt agcctttaca actttgtaat cacgttcaat
     6061 tgctatttcc attcggtcgt catttttcat tgttattccc tccgaaaata acaatatcat
     6121 attgtgactc atgcgtaaac attcaagtca cattaggaga tgatttacgt tccaaaggtc
     6181 acaataaaat tacgttctaa atctcccaat aaaagttatc aacgttccaa aagtcacaat
     6241 aaaacgttcc aaaagtcaca ataaaacgtt ccaaaggtca caataaaaac cctgcaacca
     6301 cgggctttag cgggctttcg ggtcccctaa aataataaaa ataagaaaaa taattattta
     6361 tttttacaac aaaaaaagtg actcttataa agtcactttt taaacaactt atcaatccag
     6421 gtttttgact tttctttcga caattcttct aataactcat caatttgttt tttgtaattc
     6481 gcctctgtaa gctcgtattt ttcaatttga gcgtttttat cttctaagag tatctttgtt
     6541 gcctcagctt ctgcaatttg gctttgtgcg tccattagct tgtcttgtag ctgtattatc
     6601 agttcttgcg ctttaattag cttttgacgg ctctctgtat cctctataat ttgtacaggc
     6661 gcaggcatag gatatttctt ttctagcaat tctaaggcta tatcatcaag ttctactgtt
     6721 ttaccttttt tctcagtgtg cccgttgaat ttttctggat gcctgcctat atatcgagaa
     6781 attgcctgtg gttgcacttg tctcgactct gcaaattcag taatagtcat ggtttacctc
     6841 acttttatgt acaaaatacc gtacaagtac agtatatgga acatgagcat ttttatatat
     6901 tataatatgc caaaattagg cgtttaacaa gtttttttag atatcctcgg gctgtaggtt
     6961 aaaaagtcga attcacttgg caaacgtatt tcgttgccta gtgaagttcg aatcttttta
     7021 gccgcttgag gataccacca gcttgctggt ttgactattt gaattaactt gctataatga
     7081 gaataggcaa agaaatgaga ttactatttt tgacaagcta acaaaaaaag ctaggaatgg
     7141 cagttcctag cttttttcta ttccgttttg gtcaaggtgg cttaatcctt aggctcgttg
     7201 ccattatcat cgctgtcgag ccatttgcaa atgtaatagg cagttacact tgctgcgaca
     7261 gagattagaa atgagataac tatcttcaag ctaacacccc ctttccgtgc ctagtctagg
     7321 ggtggcaacg attgaaatta tagcataatt tttagcatct tccatacaag gtatgtaaga
     7381 tcgtctgacg agctatctta caccgcaccc gtttagggtg ctcaccttgt tctcattggg
     7441 g
//
