LOCUS       40924_6541        4610 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBIOTRX-BirA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy 
            JM.
  TITLE     A thioredoxin gene fusion expression system that circumvents 
            inclusion body formation in the E. coli cytoplasm
  JOURNAL   Biotechnology (N.Y.) 11 (2), 187-193 (1993)
  PUBMED    7763371
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Smith PA, Tripp BC, DiBlasio-Smith EA, Lu Z, LaVallie ER, McCoy JM.
  TITLE     A plasmid expression system for quantitative in vivo biotinylation 
            of thioredoxin fusion proteins in Escherichia coli
  JOURNAL   Nucleic Acids Res. 26 (6), 1414-1420 (1998)
  PUBMED    9490786
REFERENCE   3  (bases 1 to 4610)
  AUTHORS   Smith PA, Tripp BC, DiBlasio-Smith EA, Lu Z, LaVallie ER, McCoy JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-1998) Molecular Biology and Gene Expression, 
            Genetics Institute, Inc., 87 Cambridge Park Drive, Cambridge, MA 
            02140, USA
REFERENCE   4  (bases 1 to 4610)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4610)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology (N.Y.)"; date: "1993"; volume: "11"; issue: "2"; 
            pages: "187-193"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998"; volume: "26"; issue: "6"; pages: 
            "1414-1420"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-1998) Molecular Biology and Gene Expression, Genetics 
            Institute, Inc., 87 Cambridge Park Drive, Cambridge, MA 02140, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2159..2187
                     /label=lambda pL
                     /note="lambda pL"
                     /regulatory_class="promoter"
     CDS             2416..2481
                     /label=lambda N peptide
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
     misc_feature    2724..2792
                     /note="Tag 81; biotinylation recognition sequence"
     CDS             2793..3119
                     /label=TrxA
                     /note="E. coli thioredoxin"
     CDS             3154..4116
                     /label=BirA
                     /note="E. coli biotin protein ligase"
     regulatory      4138..4204
                     /label=AspA gene transcription terminator
                     /note="AspA gene transcription terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(4214..4230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagaatt gatctctcac ctaccaaaca atgcccccct gcaaaaaata
     2101 aattcatata aaaaacatac agataaccat ctgcggtgat aaattatctc tggcggtgtt
     2161 gacataaata ccactggcgg tgatactgag cacatcagca ggacgcactg accaccatga
     2221 aggtgacgct cttaaaaatt aagccctgaa gaagggcagc attcaaagca gaaggctttg
     2281 gggtgtgtga tacgaaacga agcattggcc gtaagtgcga ttccggatta gctgccaatg
     2341 tgccaatcgc ggggggtttt cgttcaggac tacaactgcc acacaccacc aaagctaact
     2401 gacaggagaa tccagatgga tgcacaaaca cgccgccgcg aacgtcgcgc agagaaacag
     2461 gctcaatgga aagcagcaaa tcccctgttg gttggggtaa gcgcaaaacc agttccgaaa
     2521 gattttttta actataaacg ctgatggaag cgtttatgcg gaagaggtaa agcccttccc
     2581 gagtaacaaa aaaacaacag cataaataac cccgctctta cacattccag ccctgaaaaa
     2641 gggcatcaaa ttaaaccaca cctatggtgt atgcatttat ttgcatacat tcaatcaatt
     2701 gttatctaag gaaatactta catatggctt cttctctgcg tcaaattctg gattctcaaa
     2761 aaatggaatg gcgttctaac gctggtggtt ctatgagcga taaaattatt cacctgactg
     2821 acgacagttt tgacacggat gtactcaaag cggacggggc gatcctcgtc gatttctggg
     2881 cagagtggtg cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc gctgacgaat
     2941 atcagggcaa actgaccgtt gcaaaactga acatcgatca aaaccctggc actgcgccga
     3001 aatatggcat ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa
     3061 ccaaagtggg tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggcct
     3121 agctggccat ctagacaaga aggagatata catatgaagg ataacaccgt gccactgaaa
     3181 ttgattgccc tgttagcgaa cggtgaattt cactctggcg agcagttggg tgaaacgctg
     3241 ggaatgagcc gggcggctat taataaacac attcagacac tgcgtgactg gggcgttgat
     3301 gtctttaccg ttccgggtaa aggatacagc ctgcctgagc ctatccagtt acttaatgct
     3361 aaacagatat tgggtcagct ggatggcggt agtgtagccg tgctgccagt gattgactcc
     3421 acgaatcagt accttcttga tcgtatcgga gagcttaaat cgggcgatgc ttgcattgca
     3481 gaataccagc aggctggccg tggtcgccgg ggtcggaaat ggttttcgcc ttttggcgca
     3541 aacttatatt tgtcgatgtt ctggcgtctg gaacaaggcc cggcggcggc gattggttta
     3601 agtctggtta tcggtatcgt gatggcggaa gtattacgca agctgggtgc agataaagtt
     3661 cgtgttaaat ggcctaatga cctctatctg caggatcgca agctggcagg cattctggtg
     3721 gagctgactg gcaaaactgg cgatgcggcg caaatagtca ttggagccgg gatcaacatg
     3781 gcaatgcgcc gtgttgaaga gagtgtcgtt aatcaggggt ggatcacgct gcaggaagcg
     3841 gggatcaatc tcgatcgtaa tacgttggcg gccatgctaa tacgtgaatt acgtgctgcg
     3901 ttggaactct tcgaacaaga aggattggca ccttatctgt cgcgctggga aaagctggat
     3961 aattttatta atcgcccagt gaaacttatc attggtgata aagaaatatt tggcatttca
     4021 cgcggaatag acaaacaggg ggctttatta cttgagcagg atggaataat aaaaccctgg
     4081 atgggcggtg aaatatccct gcgtagtgca gaaaaataat ctagagtcga cctgcagtaa
     4141 tcgtacaggg tagtacaaat aaaaaaggca cgtcagatga cgtgcctttt ttcttgtgag
     4201 cagtaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     4381 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag
     4441 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4501 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4561 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
