LOCUS       40924_6402        5667 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Transient expression vector pBI221, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Chen PY, Wang CK, Soong SC, To KY.
  TITLE     Complete sequence of the binary vector pBI121 and its application in
            cloning T-DNA insertion from transgenic plants
  JOURNAL   Mol. Breed. 11, 287-293 (2003)
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Chen PY, Wang CK, To KY.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2002) Institute of Bioagricultural Sciences, 
            Academia Sinica, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 5667)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Breed.
            11, 287-293 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2002) Institute of Bioagricultural Sciences, Academia 
            Sinica, Taipei 11529, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        512..857
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             896..2704
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      2778..3030
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3039..3055)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3529..3633
                     /label=AmpR promoter
     CDS             3634..4491
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4665..5253
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5541..5562
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5577..5607
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5615..5631
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5639..5655
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
       61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
      781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
      841 ttcatttgga gagaacacgg gggactctag aggatccccg ggtggtcagt cccttatgtt
      901 acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag
      961 tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag
     1021 ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa
     1081 ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca
     1141 gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca
     1201 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt
     1261 tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac
     1321 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca
     1381 tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac
     1441 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt
     1501 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
     1561 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg
     1621 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg
     1681 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct
     1741 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg
     1801 tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg
     1861 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
     1921 tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg
     1981 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg
     2041 ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca
     2101 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg
     2161 ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
     2221 ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt
     2281 gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga
     2341 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat
     2401 caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag
     2461 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc
     2521 cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
     2581 cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt
     2641 tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa
     2701 acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac
     2761 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     2821 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     2881 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     2941 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     3001 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
     3061 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3181 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3241 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3301 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3361 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3421 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3481 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4801 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5401 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5461 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5521 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5581 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5641 ggaaacagct atgaccatga ttacgcc
//
