LOCUS       62056_3795       10630 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..687
                     /codon_start=1
                     /label=APOBEC-1
                     /note="cytidine deaminase (C to U editing enzyme) from rat"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             736..4836
                     /codon_start=1
                     /label=Cas9(D10A)
                     /note="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     promoter        4884..4918
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        4936..5011
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5919..6107
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    6212..6351
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6537..7125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        7196..7298
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7754..8554
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(8668..10086)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 atgagtagcg aaaccggtcc ggttgcagtg gatccgaccc tgcgccgtcg cattgaaccg
       61 cacgagtttg aagtgttctt tgatccgcgc gagctgcgca aagaaacttg cctgctgtac
      121 gagattaact ggggtggccg ccatagcatc tggcgccata ccagccagaa caccaataag
      181 cacgtggaag tgaattttat tgaaaaattt accaccgagc gctacttctg ccctaatacc
      241 cgctgcagca tcacctggtt tctgagctgg agcccgtgcg gcgaatgtag tcgcgccatt
      301 accgagttcc tgagccgcta tccgcatgtg accctgttca tctacatcgc ccgtctgtac
      361 catcacgccg atccgcgcaa tcgccaaggt ctgcgtgatc tgattagcag cggtgtgacc
      421 atccagatca tgaccgaaca agagagcggc tattgctggc gcaacttcgt gaactattct
      481 ccgagcaacg aagcccactg gccgcgttat ccgcatctgt gggtgcgcct gtatgtgctg
      541 gagctgtact gcatcatcct gggcctgccg ccttgcctga atattctgcg ccgtaaacag
      601 ccgcaactga cattcttcac catcgcactg cagagctgcc attatcagcg cctgccgccg
      661 cacattttat gggccaccgg tctgaaaagc ggtagtgaaa ctccgggcac aagcgaaagc
      721 gcaaccccgg aaagtgataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
      781 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
      841 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
      901 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
      961 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     1021 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     1081 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     1141 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     1201 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     1261 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     1321 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     1381 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     1441 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     1501 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     1561 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     1621 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
     1681 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     1741 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     1801 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     1861 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
     1921 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     1981 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
     2041 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
     2101 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     2161 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     2221 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     2281 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa
     2341 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     2401 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     2461 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat
     2521 ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta
     2581 gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt
     2641 aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat
     2701 actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc
     2761 aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg
     2821 atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa
     2881 ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt
     2941 attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca
     3001 gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat
     3061 tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt
     3121 aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc
     3181 caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat
     3241 gatgtcgatc acattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc
     3301 ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc
     3361 aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag
     3421 tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt
     3481 atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat
     3541 agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt
     3601 accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt
     3661 gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct
     3721 ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat
     3781 gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat
     3841 ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag
     3901 attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa
     3961 gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag
     4021 aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg
     4081 gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt
     4141 ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag
     4201 ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa
     4261 aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt
     4321 aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt
     4381 gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta
     4441 tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa
     4501 ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt
     4561 tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa
     4621 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
     4681 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
     4741 acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat
     4801 gaaacacgca ttgatttgag tcagctagga ggtgactgat ggctggttgg cgtactgttg
     4861 tggtaaatac caagcttgat atcttgacag ctagctcagt cctaggtata atactagtcg
     4921 agaccattgg tctcagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     4981 tcaacttgaa aaagtggcac cgagtcggtg ctttttttga tatcgaattc ctgcagcccg
     5041 ggggatccac tagttctaga gcggccgcca ccgcggtgga gctcatccca atggcgcgcc
     5101 gagcttggct cgagcatggt catagctgtt tcctgctcac tcattaggca ccgggatctc
     5161 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     5221 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     5281 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     5341 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     5401 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc ccacgggtgc
     5461 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
     5521 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
     5581 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
     5641 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     5701 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     5761 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     5821 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     5881 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     5941 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     6001 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     6061 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6121 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6181 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     6241 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     6301 ttgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     6361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6961 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7141 ttttctacgg ggtctgacgc tcagtggaac gcaacttttg gcgaaaatga gacgttgatc
     7201 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     7261 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     7321 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     7381 ttgctcaatg tacctataac cagaccgttc agctgggtca tccaccggat caattcccct
     7441 gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc ttggagccct
     7501 tgccctcccg cacgatgatc gtgccgtgat cgaaatccag atccttgacc cgcagttgca
     7561 aaccctcact gatccggctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc
     7621 cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc
     7681 agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg
     7741 ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa
     7801 agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt
     7861 ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc
     7921 cctgcgtgct gcgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga
     7981 cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga
     8041 cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc
     8101 ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctc actcgcctgc
     8161 aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca
     8221 cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc
     8281 gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga
     8341 ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa
     8401 ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac
     8461 agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg
     8521 cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga gaacgagatg
     8581 acgtctcgag gtcgacggta tcgatacggc atcagagcag attgtactga gagtgcacca
     8641 taatcggcat tttcttttgc gtttttattt gttaactgtt aattgtcctt gttcaaggat
     8701 gctgtctttg acaacagatg ttttcttgcc tttgatgttc agcaggaagc taggcgcaaa
     8761 cgttgattgt ttgtctgcgt agaatcctct gtttgtcata tagcttgtaa tcacgacatt
     8821 gtttcctttc gcttgaggta cagcgaagtg tgagtaagta aaggttacat cgttaggatc
     8881 aagatccatt tttaacacaa ggccagtttt gttcagcggc ttgtatgggc cagttaaaga
     8941 attagaaaca taaccaagca tgtaaatatc gttagacgta atgccgtcaa tcgtcatttt
     9001 tgatccgcgg gagtcagtga acagatacca tttgccgttc attttaaaga cgttcgcgcg
     9061 ttcaatttca tctgttactg tgttagatgc aatcagcggt ttcatcactt ttttcagtgt
     9121 gtaatcatcg tttagctcaa tcataccgag agcgccgttt gctaactcag ccgtgcgttt
     9181 tttatcgctt tgcagaagtt tttgactttc ttgacggaag aatgatgtgc ttttgccata
     9241 gtatgctttg ttaaataaag attcttcgcc ttggtagcca tcttcagttc cagtgtttgc
     9301 ttcaaatact aagtatttgt ggcctttatc ttctacgtag tgaggatctc tcagcgtatg
     9361 gttgtcgcct gagctgtagt tgccttcatc gatgaactgc tgtacatttt gatacgtttt
     9421 tccgtcaccg tcaaagattg atttataatc ctctacaccg ttgatgttca aagagctgtc
     9481 tgatgctgat acgttaactt gtgcagttgt cagtgtttgt ttgccgtaat gtttaccgga
     9541 gaaatcagtg tagaataaac ggatttttcc gtcagatgta aatgtggctg aacctgacca
     9601 ttcttgtgtt tggtctttta ggatagaatc atttgcatcg aatttgtcgc tgtctttaaa
     9661 gacgcggcca gcgtttttcc agctgtcaat agaagtttcg ccgacttttt gatagaacat
     9721 gtaaatcgat gtgtcatccg catttttagg atctccggct aatgcaaaga cgatgtggta
     9781 gccgtgatag tttgcgacag tgccgtcagc gttttgtaat ggccagctgt cccaaacgtc
     9841 caggcctttt gcagaagaga tatttttaat tgtggacgaa tcgaactcag gaacttgata
     9901 tttttcattt ttttgctgtt cagggatttg cagcatatca tggcgtgtaa tatgggaaat
     9961 gccgtatgtt tccttatatg gcttttggtt cgtttctttc gcaaacgctt gagttgcgcc
    10021 tcctgccagc agtgcggtag taaaggttaa tactgttgct tgttttgcaa actttttgat
    10081 gttcatcgtt catgtctcct tttttatgta ctgtgttagc ggtctgcttc ttccagccct
    10141 cctgtttgaa gatggcaagt tagttacgca caataaaaaa agacctaaaa tatgtaaggg
    10201 gtgacgccaa agtatacact ttgcccttta cacattttag gtcttgcctg ctttatcagt
    10261 aacaaacccg cgcgatttac ttttcgacct cattctatta gactctcgtt tggattgcaa
    10321 ctggtctatt ttcctctttt gtttgataga aaatcataaa aggatttgca gactacgggc
    10381 ctaaagaact aaaaaatcta tctgtttctt ttcattctct gtatttttta tagtttctgt
    10441 tgcatgggca taaagttgca agcttgcaaa catgctttgg gtaaacatgt aggtattaac
    10501 gtcaatgcga caatagtagc attgattaaa tgagaattag taagtgtttt acttactaaa
    10561 ttttatttaa cctaaaaatg aaccacctgg atgtgtggga ttaaaaagtg aagagaggag
    10621 gacatatcac
//
