LOCUS       V009251                 8603 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009251
VERSION     V009251
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Faries KM, Kressel LL, Wander MJ, Holten D, Laible PD, Kirmaier C,
            Hanson DK.
  TITLE     High throughput engineering to revitalize a vestigial electron
            transfer pathway in bacterial photosynthetic reaction centers
  JOURNAL   J. Biol. Chem. 287 (11), 8507-8514 (2012)
   PUBMED   22247556
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   Kressel L, Wander MJ, Hanson DK, Laible PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2011) Biosciences, Argonne National Laboratory,
            9700 S. Cass Ave., Argonne, IL 60439, USA
REFERENCE   3  (bases 1 to 8603)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8603)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "2012"; volume: "287"; issue: "11"; pages: "8507-8514"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-AUG-2011) Biosciences, Argonne National Laboratory, 9700 S. Cass
            Ave., Argonne, IL 60439, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     3162..3178
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3188..3206
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4132..4368)
                     /codon_start=1
                     /gene="pufX"
                     /product="intrinsic membrane protein PufX"
                     /label="pufX"
                     /protein_id="AEW50176.1"
                     /translation="MSMFDKPFDYENGSKFEMGIWIGRQMAYGAFLGSIPFLLGLGLVL
                     GSYGLGLMLPERAHQAPSPYTTEVVVQHATEVV"
     gene            complement(4132..4368)
                     /gene="pufX"
                     /label="pufX"
     CDS             complement(4384..4404)
                     /label="7xHis"
                     /note="6xHis affinity tag"
     CDS             complement(4405..5325)
                     /gene="pufM"
                     /label="Reaction center protein M chain"
                     /note="Reaction center protein M chain from Rhodobacter
                     capsulatus. Accession#: P11847"
     CDS             complement(5321..6166)
                     /gene="pufL"
                     /label="Reaction center protein L chain"
                     /note="Reaction center protein L chain from Rhodobacter
                     capsulatus. Accession#: P19057"
     CDS             complement(6302..6475)
                     /gene="pufA"
                     /label="Light-harvesting protein B-870 alpha chain"
                     /note="Light-harvesting protein B-870 alpha chain from
                     Rhodobacter capsulatus. Accession#: P02948"
     CDS             complement(6492..6638)
                     /gene="pufB"
                     /label="Light-harvesting protein B-870 beta chain"
                     /note="Light-harvesting protein B-870 beta chain from
                     Rhodobacter capsulatus. Accession#: P02950"
     promoter        complement(6802..6820)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6841..6857)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6865..6881)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6889..6919)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6934..6955)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(7359..8150)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggcgac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc ttcaaacagc gtcgcgccgc
     3241 cgggcatcgc ggtcaccatc tgccgcgcgc gtcgggcgcc gtcgcgcacc gggccgggca
     3301 gatgcatctc gatcccctcg gccgcggcct tgagcgccgc aaagggcgcc tgccgggcga
     3361 tggtgttcag ataatgcgcg cccccaccgg cggcgcgatc gacatgtcat tgtcgttcaa
     3421 aatcacgaac atccgcgatt tcagatggcc ggcgtggttg agcgcctcat aggccatgcc
     3481 cgcggtgatc gagccgtcgc cgatgatcgc gatggtgtca cccaccggct ggcccagttc
     3541 ccgaccgact gcaaagccca gagcggccga gatcgaggtc gaggaatggc ccgcgccaaa
     3601 ggcgtcatgc gggctctcgg agcgtttcgg aaagcccgag atccccccgg cctggcgcag
     3661 ggtcagcatg cgcgagcggc ggccggtcag gatcttgtgc ggatagcatt ggtgccccac
     3721 gtcccagatc agcttgtcgc cgggtgaatt gaagaccgca tgcagcgcga ccgtcagctc
     3781 gacgacgccc agcgaagagc caagatgccc cccggtctgg ctgacgactt cgacgatctc
     3841 gcgccggact tcggaggcga gcgccgtcag atcggcgatg ctcatcgcct tgagatcggc
     3901 aggaccggtc acgcggtcaa gatggggagt gcgggatggc gtggcgctca tggttgcgtc
     3961 ctccgatgag ctgtctggtc caaaagggtg ccgcgggggt cggacctgcg gcaccaggtg
     4021 cctgaatgaa cagggaaggg atcggggctg tggggccccg gagtttccgg gccttgaccc
     4081 ggaacggaat accccggccg cgcgacacgg gcgcggccgg ggaaagcggt gtcagacgac
     4141 ttcggtcgcg tgttgaacga cgacttcggt cgtgtagggc gagggcgcct ggtgcgcacg
     4201 ctcgggcagc atcaggccga ggccatagga acccagcacc agaccgaggc ccagcaggaa
     4261 cgggatcgag ccaaggaagg caccataggc catctgacga ccgatccaga tccccatctc
     4321 gaacttcgaa ccgttttcgt agtcgaaggg tttgtcgaac atggacatgg tttcttctcc
     4381 ttagtggtgg tggtggtggt ggtgcggggt cacgggggcg tagccgtgga cttgcgccca
     4441 gacataccag ttgtcgacaa cggtgcccga gagcaggatc ccgataccac cggtcagcgt
     4501 caccatcacg gccatccaga tcgcccagcg gtggatgcct tccatggtgg cgttgaagcc
     4561 catcgtccag cgccagaaca gggcggcgcg ttcgctagcg gtcccgcggt caacgatctg
     4621 ttccagctcg cgttcgccgc cgaaacgggt caccgcaagg atggtcgcgc cgtgcatggc
     4681 gaacaggagc gctgagccgt agagggcagc gatcgagagg ccgtggaacg ggttgtagaa
     4741 caggttgccg tggtcgagcg agaactggtt cgtccagtcg aggtgcgaga agatgccata
     4801 gggcggagca acgctccagg accccatcag gatcgggcgc cagaagccga gaaccgacca
     4861 cagccagatc gcgcttaaga aagcccaggc catgtgcttg cccatgccga gctggtcggc
     4921 ccgggtgtag acacggaccc accaggcaat gaccgatatc gccatgaaga gcgaggcgat
     4981 ctgccagaca ccaccctgtt tcagcggcgc gatggcaaga ccgtattcgg ccggcggcgg
     5041 ctccagactg aagaagaaca gatctcgcat gaagatgaag ggatcgaacc cggcctgata
     5101 ccagtaccac acaccgatgg tgaagaacca ggccgcgccg aaggccagcg agacggtgcc
     5161 ggcgatgccg agatagatcg gcccgatctg ggcgttcccc atccagccga ggatgttgaa
     5221 catccccgca ggcgtccgtt cgaaggtgtc gacgtcttcc ttgaggccca tttcgggcgc
     5281 gccggcgacc tgaacctggt taaagaagtt ctgatactca gccattgatg cctcctgcca
     5341 tgtcagccca gaacggcatg ttcacccacc agtaccacca gtcactccac agatcgaagt
     5401 agatggtacc cgagacgagc atgcagcagg ccgaccagaa caccgcgttt aaagcgagca
     5461 gcaggccgag gcggtggatg cctaacgttc cgaccgaata gcccatgagg tcacggaagt
     5521 aggtgtcctc gtggtccgga gtccgcatcg tcttgccttt gaccgggttg gcggcgctga
     5581 ggaccagagc cccgtgcatc gccagcgccc aggcggtggt gaagaacaag cttatgccca
     5641 gcatgtggaa cgggttgtag tggaagttac catatgtgta gccggtgttc gacacccagt
     5701 cgaggtgcgt ccagatacca tacgggaacg cgtagcccca cgaccccatc atcatcggac
     5761 ggatcacgac gagggtcaga taggcaaaga tggccatcga gaaggccacc ggaatgtgga
     5821 acccgatgcc gagcttgcgg cagatttcga cttcacgcag agcccagctg cagaaggcgc
     5881 ccgtcacgca gacggtgatg acctgccaaa gaccgccctt gtccagggct gccacgttca
     5941 gaccattttc caccggcggc ggaaagatgg agatcaattg ggggttccac gtgccctgca
     6001 tcgcggctcc ccaaaggata agcaaaaagc cgagcgtggc gaagaagatg gtggtgaccc
     6061 cgaagaaccc gacatagaag ggtccgaccc agaagtcgaa aaggctcccg ccaatcaagg
     6121 tgccgcccgg cacacggtat tttcgttcga aactgagcaa agccatgccc gtgtctccgc
     6181 tgttgcggga cggtccttgg cccggcaaaa ggcctcagat cgtgccgatg ggttgcgtct
     6241 tgcggtatgg tcgcgggcgg ggcgagactc tcgccccgcc cgcaacgctc agtaaggctt
     6301 actgggcagc agccacgtag ccatgcttgg cggtggcaac ggtcagccag ttgaaagcgg
     6361 gggtcgagag caggatcagc cggatcagca ccgcgagcag gaacaggaac acgccctggg
     6421 ccacgaacac gcgacgggga tcgaaaacga gccagatttt gtagaacttg gacattttca
     6481 gtttctcctc agaaccacgg acgccagatc atgaccgcca gatgggcgag caccgcgacg
     6541 gcgatgaacg ccgacagccc gctcatgtag acggcatgca gttcttgcgc ttgctcgtcg
     6601 gtaagacctg tgaagctcag gtcgttctta tcagccataa caacctccgg gttggcagac
     6661 tgacgccgca ttccctgcgg ctgggcaaac gaaatggcac ctgccatccc gcatttccac
     6721 cacgaggggc tcatcccacc cgtggagaac ttgacccgac ggggctatgg cccccgccgg
     6781 aattcgatcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     6841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc atgcataaaa actgttgtaa
     7081 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     7141 cagcggcatc agcaccttgt cgccttgcgt ataatatttg ccggggtggg cgaagaactc
     7201 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     7261 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     7321 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     7381 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     7441 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     7501 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     7561 atgatattcg gcaagcaggc atcgccatgc gtcacgacga gatcctcgcc gtcgggcatg
     7621 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     7681 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     7741 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     7801 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     7861 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     7921 caaggaacgc ccgtcgtggc gagccacgat agccgcgctg cctcgtcctg aagttcattc
     7981 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     8041 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     8101 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     8161 cctcatcctg tctcttgatc attgatcccc tgcgccatca gatccttggc ggcaagaaag
     8221 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     8281 ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc
     8341 aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
     8401 cattcatccc aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg
     8461 ggcctgtttc tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt
     8521 gatgatcgcg gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac
     8581 gggcttcagg cgctcccgaa ggt
//
