LOCUS       62056_3735        5655 bp DNA     circular SYN 06-DEC-2019
DEFINITION  Cloning vector pBBR4pemIK, complete sequence.
ACCESSION   MN044101
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Burbank L, Wei W.
  TITLE     Broad-Host-Range Plasmids for Constitutive and Inducible Gene 
            Expression in the Absence of Antibiotic Selection
  JOURNAL   Microbiol Resour Announc 8 (36), e00769-19 (2019)
  PUBMED    31488530
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Burbank LP, Wei W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2019) Crop Diseases Pests and Genetics Research 
            Unit, USDA Agricultural Research Service, 9611 S Riverbend Ave, 
            Parlier, CA 93648, USA
REFERENCE   3  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc"; date: "2019"; volume: "8"; issue: "36"; pages: 
            "e00769-19"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2019) Crop Diseases Pests and Genetics Research Unit, USDA 
            Agricultural Research Service, 9611 S Riverbend Ave, Parlier, CA 
            93648, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..770
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             771..1430
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     2140..2156
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2166..2184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2217..2233
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2293..2655)
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase PemK"
                     /label=mRNA interferase PemK
                     /protein_id="QDJ95324.1"
                     /translation="MGRCSSSRTRTDMKRGDVYMVDLEPTAGHEQRGHRPVVVISSERF
                     NRLTSCPVILPITNGGEFASRLGFAVELLGTITTGVVRCDQPRALDLLARNARKVESLP
                     PNILADVLAKAVTIFQ"
     CDS             complement(2616..2879)
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin PemI"
                     /label=antitoxin PemI
                     /protein_id="QDJ95325.1"
                     /translation="MHTTNLRKVGGSIMLAVPPAFLDQLHLEVGATVGLAVTDGRLVIE
                     PVLHPQYTLDQLLAEAEASGAYPLPPEEREWVDAPAVGRELI"
     primer_bind     complement(2972..2988)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3018..3036)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3057..3073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3081..3097)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3105..3135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3150..3171)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3399..3470
                     /label=AmpR promoter
     CDS             3471..4328
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc cgcccgcctg
      361 ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca
      421 aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg
      481 gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa
      541 ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg
      601 caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta
      661 attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg
      721 attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc atggccacgc
      781 agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac
      841 gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc
      901 tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac
      961 tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg
     1021 ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg
     1081 tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg
     1141 ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc
     1201 gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc
     1261 cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg
     1321 aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg
     1381 acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcacttgg
     1441 gttgcgcagc aacccgtaag tgcgctgttc cagactatcg gctgtagccg cctcgccgcc
     1501 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc
     1561 tgaatggaag ccggcggcac ctcgctaacg gattcaccgt ttttatcagg ctctgggagg
     1621 cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc
     1681 ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
     1741 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt
     1801 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc
     1861 gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag
     1921 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1981 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2041 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     2101 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     2161 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
     2221 gaactagtgg atcccccggg ctgcaggaat tcactagtga ttggttgcag tggcctgaac
     2281 ttgccccaaa aattactgga agatggtgac agcttttgcc aagacatcgg ccaagatgtt
     2341 tggtggtagg ctttccactt tgcgggcatt gcgagcgagt aagtcgagcg cacgtggttg
     2401 atcacatcga acgacgcccg ttgtaatcgt acctagtaat tccacagcga acccgaggcg
     2461 ggaggcgaac tcgccgccgt ttgtgattgg aaggatcacc ggacaagagg taagccgatt
     2521 gaatcgctca ctggagataa caaccaccgg acggtgaccg cgttgttcgt gtccggcggt
     2581 tggctccaga tcgaccatgt aaacatcgcc gcgtttcata tcagttcgcg tcctactgct
     2641 ggagcatcga cccattcacg ttcctcgggc ggcaacgggt aagccccgga ggcttccgct
     2701 tcggccagca gttgatccag cgtgtactga ggatgtagga ctggctcaat gaccagacga
     2761 ccgtctgtga cggctaagcc gaccgttgcg ccgacctcaa gatgaagctg atcaaggaaa
     2821 gcggggggaa cggccagcat gatcgatcca ccgactttac gaaggtttgt ggtgtgcatg
     2881 atgggcttct ttcgttaagt tatattaaag tataatataa ttcctccatt tatacgctta
     2941 tagcgaaaat cgaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
     3001 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     3061 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgcatgc ataaaaactg ttgtaattca
     3301 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     3361 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca ttcaaatatg tatccgctca
     3421 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3481 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3541 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3601 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3661 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3721 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3781 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3841 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3901 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3961 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4021 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4081 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4141 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4201 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4261 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4321 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4381 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4441 aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg ggcctgtttc
     4501 tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt gatgatcgcg
     4561 gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac gggcttcagg
     4621 cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg catctcacgc
     4681 gctcctgcgg cggcctgtag ggcaggctca tacccctgcc gaaccgcttt tgtcagccgg
     4741 tcggccacgg cttccggcgt ctcaacgcgc tttgagattc ccagcttttc ggccaatccc
     4801 tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg gcccactggt
     4861 ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt gctgccctcg
     4921 atgccccgtt gcagccctag atcggccaca gcggccgcaa acgtggtctg gtcgcgggtc
     4981 atctgcgctt tgttgccgat gaactccttg gccgacagcc tgccgtcctg cgtcagcggc
     5041 accacgaacg cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc cgtcacgatg
     5101 cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa cgccgcctgc
     5161 tgttcttggc tggccgactt ccaccattcc gggctggccg tcatgacgta ctcgaccgcc
     5221 aacacagcgt ccttgcgccg cttctctggc agcaactcgc gcagtcggcc catcgcttca
     5281 tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc agcgttgggc
     5341 gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat tttcgccagc
     5401 ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg gtgtccaacc
     5461 ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc cactcaatgc ttgagtatac
     5521 tcactagact ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc
     5581 ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt
     5641 ggacgatggc cgcga
//
