LOCUS       40924_6082       10044 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Site-specific excision vector pBBR1_FLP2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Jittawuttipoka T, Buranajitpakorn S, Fuangthong M, Schweizer HP, 
            Vattanaviboon P, Mongkolsuk S.
  TITLE     Mini-Tn7 vectors as genetic tools for gene cloning at a single copy 
            number in an industrially important and phytopathogenic bacteria, 
            Xanthomonas spp
  JOURNAL   FEMS Microbiol. Lett. 298 (1), 111-117 (2009)
  PUBMED    19659730
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   Jittawuttipoka T, Buranajitpakorn S, Fuangthong M, Schweizer HP, 
            Vattanaviboon P, Mongkolsuk S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Laboratory of Biotechnology, Chulabhorn 
            Research Institute, 54 Vibhavadee Rangsit Hwy, Bangko 10210, 
            Thailand
REFERENCE   3  (bases 1 to 10044)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2009"; volume: "298"; issue: "1"; pages: 
            "111-117"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2009) Laboratory of Biotechnology, Chulabhorn Research 
            Institute, 54 Vibhavadee Rangsit Hwy, Bangko 10210, Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3239..3255
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3979..5397)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(5398..5843)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             complement(6067..7335)
                     /codon_start=1
                     /label=FLP
                     /note="site-specific recombinase"
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     RBS             7339..7347
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7436..8146
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     promoter        complement(8429..8447)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8468..8484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8492..8508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8516..8546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8561..8582)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8810..8881
                     /label=AmpR promoter
     CDS             8882..9739
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     3241 tagaactagt ggatcccccc ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc
     3301 agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc cgcattatgg
     3361 gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt cggtaacctc
     3421 gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggcta
     3481 tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc tccggaagac
     3541 ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct
     3601 gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg
     3661 cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa
     3721 cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc
     3781 ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt
     3841 tggagtcatt accaaaaggt taggaatacg gttagccatt tgcctgcttt tatatagttc
     3901 atatgggatt cacctttatg ttgataagaa ataaaagaaa atgccaatag gatatcggca
     3961 ttttcttttg cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt
     4021 gacaacagat gttttcttgc ctttgatgtt cagcaggaag ctaggcgcaa acgttgattg
     4081 tttgtctgcg tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt
     4141 cgcttgaggt acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat
     4201 ttttaacaca aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac
     4261 ataaccaagc atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg
     4321 ggagtcagtg aacagatacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc
     4381 atctgttact gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc
     4441 gtttagctca atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct
     4501 ttgcagaagt ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt
     4561 gttaaataaa gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac
     4621 taagtatttg tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc
     4681 tgagctgtag ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc
     4741 gtcaaagatt gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga
     4801 tacgttaact tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt
     4861 gtagaataaa cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt
     4921 ttggtctttt aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc
     4981 agcgtttttc cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga
     5041 tgtgtcatcc gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata
     5101 gtttgcgaca gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt
     5161 tgcagaagag atatttttaa ttgtggacga atcgaactca ggaacttgat atttttcatt
     5221 tttttgctgt tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt
     5281 ttccttatat ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag
     5341 cagtgcggta gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt
     5401 tcatgtctcc ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga
     5461 agatggcaag ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca
     5521 aagtatacac tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc
     5581 gcgcgattta cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat
     5641 tttcctcttt tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac
     5701 taaaaaatct atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc
     5761 ataaagttgc ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata
     5821 atagtgaacg gcaggtatat gtgatgggtt aaaaaggatc gatcctctag aggatccccc
     5881 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
     5941 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
     6001 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg
     6061 tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat
     6121 cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg
     6181 ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg
     6241 atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg
     6301 atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac
     6361 attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat
     6421 gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc
     6481 tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga
     6541 agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc
     6601 caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac
     6661 gcttgtcttt gtctctgtca ctaaacactg gattattact cccagatact tattttggac
     6721 taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa
     6781 agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc
     6841 aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc
     6901 tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg
     6961 taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg
     7021 aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca gaattgttgc
     7081 tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcagcgaatt
     7141 gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt
     7201 aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg
     7261 tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat
     7321 accaaattgt ggcatacaac ctccttagta catgcaacca ttatcaccgc cagaggtaaa
     7381 atagtcaaca cgcacggtgt tagatattta tcccttgcgg tgatagattt aacgtatgag
     7441 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
     7501 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
     7561 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
     7621 cgccgcattg cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
     7681 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
     7741 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt
     7801 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
     7861 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
     7921 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
     7981 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
     8041 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
     8101 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat cggcaaggtg
     8161 ttctggtcgg cgcatagctg ataacaattg agcaagaatc ttcatcgaat taggggaatt
     8221 ttcactcccc tcagaacata acatagtaaa tggattgaat tatgaagaat ggtttttatg
     8281 cgacttaccg cagcaaaaat aaagggaaag ataagcgctc aataaacctg tctgttttcc
     8341 ttaattctct gctggctgat aatcatcacc tgcaggttgg ctccaattat ttgtatattc
     8401 ataaaatcgg ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     8461 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8521 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgcatg cataaaaact
     8701 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     8761 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc attcaaatat
     8821 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8881 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8941 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9001 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9061 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9121 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9181 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9241 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9301 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9361 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9421 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9481 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9541 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9601 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9661 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9721 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9781 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9841 gaccaaaatc caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
     9901 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
     9961 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
    10021 cgggcttcag gcgctcccga aggt
//
