LOCUS       V013861                 6765 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013861
VERSION     V013861
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1657..1678
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1792..1810
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1840..1856
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(1910..1987)
                     /label="lacI promoter"
     promoter        2033..2061
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             2108..3352
                     /gene="ndh"
                     /label="Type II NADH:quinone oxidoreductase"
                     /note="Type II NADH:quinone oxidoreductase from Escherichia
                     coli (strain K12). Accession#: P00393"
     CDS             3353..3385
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      3422..3469
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(3511..3527)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(3560..3578)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3588..3604)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4314..4973)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(4974..5743)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
     1861 gcttgatatc gaattcgagc tcggtacctc gcgaatgcat ctagatcaca ttcaccaccc
     1921 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     1981 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa atttgacaat
     2041 taatcatcgg ctcgtataat gtgtggaatg tctcatgagc ggatatttca cacaggaaac
     2101 agtattcatg gcaacacagc tggggcataa gctgggacgc aagaaaaaag ccaaaattac
     2161 gctggtcgat cgtaaccaca gccacctgtg gaaaccgctg ctgcacgaag tggcgactgg
     2221 ctcgcttgat gaaggcgtcg atgcgttgag ctatctggcc catgcgcgca atcatggttt
     2281 ccagttccag ctgggttccg tcattgatat tgatcgtgaa gcgaaaacaa tcactattgc
     2341 agaactgcgc gacgagaaag gtgaactgct ggttccggaa cgtaaaatcg cctatgacac
     2401 cctggtaatg gcgctgggta gcacctctaa cgatttcaat acgccaggtg tcaaagagaa
     2461 ctgcattttc ctcgataacc cgcaccaggc gcgtcgcttc caccaggaga tgctgaattt
     2521 gttcctgaaa tactccgcca acctgggcgc gaatggcaaa gtgaacattg cgattgtcgg
     2581 cggcggcgcg acgggtgtag aactctccgc tgaattgcac aacgcggtca agcaactgca
     2641 cagctacggt tacaaaggcc tgaccaacga agccctgaac gtaacgctgg tagaagcggg
     2701 agaacgtatt ttgcctgcgt taccgccacg tatctctgct gcggcccaca acgagctaac
     2761 gaaacttggc gttcgcgtgc tgacgcaaac catggtcacc agtgctgatg aaggcggcct
     2821 gcacactaaa gatggcgaat atattgaggc tgatctgatg gtatgggcag ccgggatcaa
     2881 agcgccagac ttcctgaaag atatcggtgg tcttgaaact aaccgtatca accagctggt
     2941 ggtggaaccg acgctgcaaa ccacccgcga tccagacatt tacgctattg gcgactgcgc
     3001 gtcatgcccg cgtccggaag ggggctttgt tccgccgcgt gctcaggctg cacaccagat
     3061 ggcgacttgc gcaatgaaca acattctggc gcagatgaac ggtaagccgc tgaaaaatta
     3121 tcagtataaa gatcatggtt cgctggtatc gctgtcgaac ttctccaccg tcggtagcct
     3181 gatgggtaac ctgacgcgcg gctcaatgat gattgaagga cgaattgcgc gctttgtata
     3241 tatctcgcta taccgaatgc atcagattgc gctgcatggt tactttaaaa ccggattaat
     3301 gatgctggtg gggagtatta accgcgttat ccgtccgcgt ttgaagttgc atgcagcgaa
     3361 cgacgaaaat tacgcccttg cagcgtaatt aacctaggct gctgccaccg ctgagcaata
     3421 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
     3481 ggcatttgag aagcacacgg tcacacatcg gatccactag ttctagagcg gccgccaccg
     3541 cggtggagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3601 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3661 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3721 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     3781 taaatttttg ttaaatcagc tcatttttta accaataggc cgactgcgat gagtggcagg
     3841 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg
     3901 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
     3961 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
     4021 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
     4081 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
     4141 aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
     4201 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg aggcggctac
     4261 agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc
     4321 ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca
     4381 tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct
     4441 ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg
     4501 gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc
     4561 aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg
     4621 cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccgacacg
     4681 gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact
     4741 gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc
     4801 accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg
     4861 ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag
     4921 tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc
     4981 tccatgcggt agggtgccgc acggttgcgg caccatgcgc aatcagctgc aacttttcgg
     5041 cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat tttctttaac
     5101 ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc ccctttttta
     5161 agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg gtgcccaaag
     5221 aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt
     5281 ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg
     5341 cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg
     5401 ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa ggccaagccg
     5461 ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac cggcaagcga
     5521 agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa ttgatggggc
     5581 aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg ggtagccggt
     5641 gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc ttgtccagca
     5701 gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc catcgtccac
     5761 atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg gagagcaagc
     5821 ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag tctagtgagt
     5881 atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc gtcgagccgg
     5941 ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga tgcaagaagc
     6001 tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag cgcgagacgc
     6061 ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc agcaccgatg
     6121 aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac gctgtgttgg
     6181 cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc caagaacagc
     6241 aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg gcggatcgca
     6301 tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg ttcgtggtgc
     6361 cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa gcgcagatga
     6421 cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa cggggcatcg
     6481 agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg gagcggccac
     6541 cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat gcaccgcagg
     6601 gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc gtggccgacc
     6661 ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc gcaggagcgc
     6721 gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgag
//
