LOCUS       40924_5789        4613 bp DNA     circular SYN 16-OCT-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     misc_feature    306..362
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      565..651
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      743..770
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        789..880
                     /label=AmpR promoter
     CDS             881..1738
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1783..2238
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2349..2937
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3123..3263)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3712..4587)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
      361 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      421 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      481 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      541 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      601 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
      661 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
      721 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
      781 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      841 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      901 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      961 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1021 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1081 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     1141 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1201 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1261 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1321 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1381 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     1441 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1501 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1561 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1621 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1681 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1741 actgtcagac caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg
     1801 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1861 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1921 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1981 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     2041 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt
     2101 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     2161 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     2221 tattaacgtt tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2461 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3001 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3061 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3121 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3181 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     3241 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3301 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3361 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcaagga gatggcgccc
     3421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     3481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     3541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt
     3601 ttgacagctt atcatcgatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
     3661 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
     3721 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
     3781 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
     3841 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
     3901 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
     3961 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
     4021 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
     4081 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
     4141 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
     4201 atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca
     4261 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
     4321 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
     4381 acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
     4441 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
     4501 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
     4561 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gag
//
