LOCUS       62056_3220       15899 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15899)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        107..530
                     /label=AtU6-26
                     /note="Arabidopsis U6-26 gene promoter"
     CDS             810..1598
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1753..1828
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2442..2786
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2961..3677
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3798..7898
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     terminator      7997..8249
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8365..9042
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             9110..10132
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10175..10349
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(10499..10523)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(10802..10829)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(10921..11007)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             11176..11802
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12239..13303
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      13366..13560
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    13904..14044
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(14230..14818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14865..15671)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagctcacg tgacggaatt aagcttcgac ttgccttccg
      121 cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt
      181 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
      241 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
      301 ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa
      361 aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag
      421 tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag
      481 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gggagaccaa
      541 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      601 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      661 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      721 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      781 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
      841 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      901 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      961 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1021 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1081 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1141 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1201 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1261 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1321 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1381 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1441 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1501 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1561 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1621 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1681 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1741 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1801 acttgaaaaa gtggcaccga gtcggtgctt ttttttgcaa aattttccag atcgatttct
     1861 tcttcctctg ttcttcggcg ttcaatttct ggggttttct cttcgttttc tgtaactgaa
     1921 acctaaaatt tgacctaaaa aaaatctcaa ataatatgat tcagtggttt tgtacttttc
     1981 agttagttga gttttgcagt tccgatgaga taaaccaata agcttgcatg cctgcaggtc
     2041 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
     2101 gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     2161 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     2221 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     2281 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     2341 tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact tgtctactcc
     2401 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
     2461 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
     2521 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
     2581 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     2641 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
     2701 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     2761 ggaagttcat ttcatttgga gaggacctcg acctcaacac aacatataca aaacaaacga
     2821 atctcaagca atcaagcatt ctacttctat tgcagcaatt taaatcattt cttttaaagc
     2881 aaaagcaatt ttctgaaaat tttcaccatt tacgaacgat actcgagggg gatccccaat
     2941 acttgtatgg ccgcggccgc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3001 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3061 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3121 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     3181 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3241 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3301 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3361 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3421 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     3481 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3541 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     3601 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3661 tggacgagct gtacaagtgc gattacaagg accacgacgg ggattacaag gaccacgaca
     3721 ttgattacaa ggatgatgat gacaagatgg ctccgaagaa gaagaggaag gttggcatcc
     3781 acggggtgcc agctgctgac aagaagtact cgatcggcct cgatattggg actaactctg
     3841 ttggctgggc cgtgatcacc gacgagtaca aggtgccctc aaagaagttc aaggtcctgg
     3901 gcaacaccga tcggcattcc atcaagaaga atctcattgg cgctctcctg ttcgacagcg
     3961 gcgagacggc tgaggctacg cggctcaagc gcaccgcccg caggcggtac acgcgcagga
     4021 agaatcgcat ctgctacctg caggagattt tctccaacga gatggcgaag gttgacgatt
     4081 ctttcttcca caggctggag gagtcattcc tcgtggagga ggataagaag cacgagcggc
     4141 atccaatctt cggcaacatt gtcgacgagg ttgcctacca cgagaagtac cctacgatct
     4201 accatctgcg gaagaagctc gtggactcca cagataaggc ggacctccgc ctgatctacc
     4261 tcgctctggc ccacatgatt aagttcaggg gccatttcct gatcgagggg gatctcaacc
     4321 cggacaatag cgatgttgac aagctgttca tccagctcgt gcagacgtac aaccagctct
     4381 tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa ggctatcctg tccgctaggc
     4441 tctcgaagtc tcggcgcctc gagaacctga tcgcccagct gccgggcgag aagaagaacg
     4501 gcctgttcgg gaatctcatt gcgctcagcc tggggctcac gcccaacttc aagtcgaatt
     4561 tcgatctcgc tgaggacgcc aagctgcagc tctccaagga cacatacgac gatgacctgg
     4621 ataacctcct ggcccagatc ggcgatcagt acgcggacct gttcctcgct gccaagaatc
     4681 tgtcggacgc catcctcctg tctgatattc tcagggtgaa caccgagatt acgaaggctc
     4741 cgctctcagc ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga
     4801 aggcgctggt caggcagcag ctccccgaga agtacaagga gatcttcttc gatcagtcga
     4861 agaacggcta cgctgggtac attgacggcg gggcctctca ggaggagttc tacaagttca
     4921 tcaagccgat tctggagaag atggacggca cggaggagct gctggtgaag ctcaatcgcg
     4981 aggacctcct gaggaagcag cggacattcg ataacggcag catcccacac cagattcatc
     5041 tcggggagct gcacgctatc ctgaggaggc aggaggactt ctaccctttc ctcaaggata
     5101 accgcgagaa gatcgagaag attctgactt tcaggatccc gtactacgtc ggcccactcg
     5161 ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc agaggagacg atcacgccgt
     5221 ggaacttcga ggaggtggtc gacaagggcg ctagcgctca gtcgttcatc gagaggatga
     5281 cgaatttcga caagaacctg ccaaatgaga aggtgctccc taagcactcg ctcctgtacg
     5341 agtacttcac agtctacaac gagctgacta aggtgaagta tgtgaccgag ggcatgagga
     5401 agccggcttt cctgtctggg gagcagaaga aggccatcgt ggacctcctg ttcaagacca
     5461 accggaaggt cacggttaag cagctcaagg aggactactt caagaagatt gagtgcttcg
     5521 attcggtcga gatctctggc gttgaggacc gcttcaacgc ctccctgggg acctaccacg
     5581 atctcctgaa gatcattaag gataaggact tcctggacaa cgaggagaat gaggatatcc
     5641 tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg ggagatgatc gaggagcgcc
     5701 tgaagactta cgcccatctc ttcgatgaca aggtcatgaa gcagctcaag aggaggaggt
     5761 acaccggctg ggggaggctg agcaggaagc tcatcaacgg cattcgggac aagcagtccg
     5821 ggaagacgat cctcgacttc ctgaagagcg atggcttcgc gaaccgcaat ttcatgcagc
     5881 tgattcacga tgacagcctc acattcaagg aggatatcca gaaggctcag gtgagcggcc
     5941 agggggactc gctgcacgag catatcgcga acctcgctgg ctcgccagct atcaagaagg
     6001 ggattctgca gaccgtgaag gttgtggacg agctggtgaa ggtcatgggc aggcacaagc
     6061 ctgagaacat cgtcattgag atggcccggg agaatcagac cacgcagaag ggccagaaga
     6121 actcacgcga gaggatgaag aggatcgagg agggcattaa ggagctgggg tcccagatcc
     6181 tcaaggagca cccggtggag aacacgcagc tgcagaatga gaagctctac ctgtactacc
     6241 tccagaatgg ccgcgatatg tatgtggacc aggagctgga tattaacagg ctcagcgatt
     6301 acgacgtcga tcatatcgtt ccacagtcat tcctgaagga tgactccatt gacaacaagg
     6361 tcctcaccag gtcggacaag aaccggggca agtctgataa tgttccttca gaggaggtcg
     6421 ttaagaagat gaagaactac tggcgccagc tcctgaatgc caagctgatc acgcagcgga
     6481 agttcgataa cctcacaaag gctgagaggg gcgggctctc tgagctggac aaggcgggct
     6541 tcatcaagag gcagctggtc gagacacggc agatcactaa gcacgttgcg cagattctcg
     6601 actcacggat gaacactaag tacgatgaga atgacaagct gatccgcgag gtgaaggtca
     6661 tcaccctgaa gtcaaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc
     6721 gggagatcaa caattaccac catgcccatg acgcgtacct gaacgcggtg gtcggcacag
     6781 ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt gtacggggac tacaaggttt
     6841 acgatgtgag gaagatgatc gccaagtcgg agcaggagat tggcaaggct accgccaagt
     6901 acttcttcta ctctaacatt atgaatttct tcaagacaga gatcactctg gccaatggcg
     6961 agatccggaa gcgccccctc atcgagacga acggcgagac gggggagatc gtgtgggaca
     7021 agggcaggga tttcgcgacc gtcaggaagg ttctctccat gccacaagtg aatatcgtca
     7081 agaagacaga ggtccagact ggcgggttct ctaaggagtc aattctgcct aagcggaaca
     7141 gcgacaagct catcgcccgc aagaaggact gggatccgaa gaagtacggc gggttcgaca
     7201 gccccactgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga
     7261 agctcaagag cgtgaaggag ctgctgggga tcacgattat ggagcgctcc agcttcgaga
     7321 agaacccgat cgatttcctg gaggcgaagg gctacaagga ggtgaagaag gacctgatca
     7381 ttaagctccc caagtactca ctcttcgagc tggagaacgg caggaagcgg atgctggctt
     7441 ccgctggcga gctgcagaag gggaacgagc tggctctgcc gtccaagtat gtgaacttcc
     7501 tctacctggc ctcccactac gagaagctca agggcagccc cgaggacaac gagcagaagc
     7561 agctgttcgt cgagcagcac aagcattacc tcgacgagat cattgagcag atttccgagt
     7621 tctccaagcg cgtgatcctg gccgacgcga atctggataa ggtcctctcc gcgtacaaca
     7681 agcaccgcga caagccaatc agggagcagg ctgagaatat cattcatctc ttcaccctga
     7741 cgaacctcgg cgcccctgct gctttcaagt acttcgacac aactatcgat cgcaagaggt
     7801 acacaagcac taaggaggtc ctggacgcga ccctcatcca ccagtcgatt accggcctct
     7861 acgagacgcg catcgacctg tctcagctcg ggggcgacaa gcggccagcg gcgacgaaga
     7921 aggcggggca ggcgaagaag aagaagtgag ctcaggcctc catacaagta ttggggatcc
     7981 actagcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     8041 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     8101 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     8161 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     8221 gcgcgcggtg tcatctatgt tactagatcg gaattcgagg cggtttgcgt attggctaga
     8281 gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     8341 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     8401 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     8461 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
     8521 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     8581 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct
     8641 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
     8701 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     8761 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
     8821 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     8881 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     8941 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagaccttcc
     9001 tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct
     9061 acaaatctat ctctctcgag ctttcgcaga tcccgggggg caatgagata tgaaaaagcc
     9121 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     9181 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     9241 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     9301 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagtttag
     9361 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     9421 tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg cgatcgctgc
     9481 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     9541 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     9601 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     9661 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     9721 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     9781 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     9841 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac gactccgggc
     9901 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     9961 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
    10021 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
    10081 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga aatagagtag
    10141 atgccgaccg gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
    10201 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
    10261 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
    10321 aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca
    10381 ttaaaaacgt ccgcaatgtg ttattaagtt gtcactagtc aggttaactc aattcggcgt
    10441 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
    10501 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
    10561 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
    10621 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
    10681 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
    10741 gagcgttgct gcctgtgatc acttaagtaa ctaactaaca ggaagagttt gtagaaacgc
    10801 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
    10861 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
    10921 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
    10981 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcttagtagt
    11041 tagacgtccc cgagatccat gctagaccat gaatccagaa gcccgagagg ttgccgcctt
    11101 tcgggctttt tctttttcaa aaaaaaaaat ttataaaacg atctgttgcg gccggccgcc
    11161 gggttgtggg caaagatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
    11221 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
    11281 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
    11341 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
    11401 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
    11461 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
    11521 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
    11581 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
    11641 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
    11701 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
    11761 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
    11821 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
    11881 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
    11941 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
    12001 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
    12061 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
    12121 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
    12181 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg
    12241 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
    12301 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
    12361 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
    12421 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
    12481 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
    12541 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
    12601 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
    12661 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
    12721 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
    12781 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
    12841 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
    12901 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
    12961 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
    13021 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
    13081 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
    13141 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
    13201 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
    13261 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
    13321 gctagggcaa attgccctag caggggaaaa aggtcgaaaa tttcctgtgg atagcacgta
    13381 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    13441 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    13501 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    13561 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    13621 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    13681 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    13741 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    13801 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    13861 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    13921 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    13981 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    14041 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    14101 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    14161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    14221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    14281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    14341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    14401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    14461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    14521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    14581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    14641 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    14701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    14761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaagg
    14821 gcccaatctg aataatgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
    14881 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
    14941 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
    15001 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
    15061 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
    15121 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
    15181 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
    15241 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcagggac
    15301 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
    15361 gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
    15421 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
    15481 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
    15541 ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
    15601 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga
    15661 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
    15721 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
    15781 tgcagtttga tcccgagggg aaccctgtgg ttgacatgca catacaaatg gacgaacgga
    15841 taaacctttt cacgcccttt taaatatccg ttattctaat aaacgctctt ttctcttag
//
