LOCUS       40924_4939        3334 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing AsRed2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..984
                     /codon_start=1
                     /label=AsRed2
                     /note="Anemonia sulcata red fluorescent protein"
                     /translation="MASLLKKTMPFRTTIEGTVNGHYFKCTGKGEGNPLEGTQEMKIEV
                     IEGGPLPFAFHILSTSCMYGSKAFIKYVSGIPDYFKQSLPEGFTWERTTTYEDGGFLTA
                     HQDTSLDGDCLVYKVKILGNNFPADGPVMQNKAGRWEPSTEIVYEVDGVLRGQSLMALE
                     CPGGRHLTCHLHTTYRSKKPASALKMPGFHFEDHRIEILEEVEKGKCYKQYEAAVGRYC
                     DAAPSKLGHN"
     promoter        1429..1533
                     /label=AmpR promoter
     CDS             1534..2391
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2565..3153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggcctctttg
      301 ctgaagaaga ccatgccctt caggaccacc atcgagggca ccgtgaacgg ccactacttc
      361 aagtgcaccg gcaagggcga gggcaacccc ctggagggca cccaggagat gaagatcgag
      421 gtgatcgagg gcggccccct gcccttcgcc ttccacatcc tgtccacctc ctgcatgtac
      481 ggctccaagg ccttcatcaa gtacgtgtcc ggcatccccg actacttcaa gcagtccctc
      541 cccgagggct tcacctggga gcgcaccacc acctacgagg acggcggctt cctgaccgcc
      601 caccaggaca cctccctgga cggcgactgc ctggtgtaca aggtgaagat cctgggcaac
      661 aacttccccg ccgacggccc cgtgatgcag aacaaggccg gccgctggga gccctccacc
      721 gagatcgtgt acgaggtgga cggcgtgctg cgcggccagt ccctgatggc cctggagtgc
      781 cccggcggtc gccacctgac ctgccacctg cacaccacct accgctccaa gaagcccgcc
      841 tccgccctga agatgcccgg cttccacttc gaggaccacc gcatcgagat cctggaggag
      901 gtggagaagg gcaagtgcta caagcagtac gaggccgccg tgggccgcta ctgcgacgcc
      961 gccccctcca agctgggcca caactgaagc ggccgcgact ctagaattcc aactgagcgc
     1021 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggcctact agtcggccgt
     1081 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     1141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     1201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     1261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     1321 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     1381 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1501 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1561 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1621 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1681 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1741 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1801 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1861 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1921 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1981 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2041 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2101 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2161 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2221 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2281 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2341 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2401 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2461 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2521 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2581 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2641 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2701 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2761 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2821 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2881 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2941 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3001 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3061 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3121 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3181 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3241 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3301 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
