LOCUS       Exported                5042 bp DNA     circular SYN 16-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4211..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..35)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(215..643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        670..774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             775..1635
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1806..2394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2682..2703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2718..2748
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2756..2772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2780..2796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2827..2845
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3863..4208
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4211..4263
                     /label=MCS
     terminator      4267..4973
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(5014..5032)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
       61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat
      181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
      241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
      301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
      421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
      481 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
      541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
      601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
      661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt tggccaagtc
     2821 ggcctctaat acgactcact atagggagct cgtcgagcgg ccgctcgacg aattaattcc
     2881 aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat
     2941 tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga
     3001 tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt
     3061 tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga
     3121 caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc
     3181 cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag
     3241 cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct
     3301 ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac
     3361 tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag
     3421 agaggcctac gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat
     3481 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa
     3541 gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca
     3601 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc
     3661 tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg
     3721 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa
     3781 tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag
     3841 tctcagaaga ccaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc
     3901 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg
     3961 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg
     4021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     4081 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
     4141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     4201 agaggacacg ctcgaggaat tcggtacccc gggttcgaaa tcgataagct tggatcctct
     4261 agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat
     4321 tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt
     4381 cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc
     4441 gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat
     4501 attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg
     4561 cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga
     4621 ctgattacat aaatcttatt caaatttcaa aaggccccag gggctagtat ctacgacaca
     4681 ccgagcggcg aactaataac gttcactgaa gggaactccg gttccccgcc ggcgcgcatg
     4741 ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat
     4801 tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca
     4861 tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa
     4921 atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggacctgc
     4981 aggcatgcaa gctagcttac tagtgatgca tattctatag tgtcacctaa atctgcggcc
     5041 gc
//
