LOCUS       40924_4774       11667 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(477..501)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    757..778
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        793..823
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    831..847
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     855..871
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        889..907
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(1027..1045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1052..1068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1226..1409
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1410..2228
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     terminator      2856..3108
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(3230..3254)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            3808..3917
                     /label=oriT
                     /note="incP origin of transfer"
     mobile_element  3977..4744
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             4744..5094
                     /label=traJ
                     /note="oriT-recognizing protein"
     CDS             5369..6514
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(7877..8465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             9562..10350
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=aadA
                     /note="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      complement(10955..11667)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcgacatctt gctgcgttcg gatattttcg tggagttccc gccacagacc cggattgaag
       61 gcgagatcca gcaactcgcg ccagatcatc ctgtgacgga actttggcgc gtgatgactg
      121 gccaggacgt cggccgaaag agcgacaagc agatcacgat tttcgacagc gtcggatttg
      181 cgatcgagga tttttcggcg ctgcgctacg tccgcgaccg cgttgaggga tcaagccaca
      241 gcagcccact cgaccttcta gccgacccag acgagccaag ggatcttttt ggaatgctgc
      301 tccgtcgtca ggctttccga cgtttgggtg gttgaacaga agtcattatc gtacggaatg
      361 ccagcactcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
      421 accttttcac gcccttttaa atatccgtta ttctaataaa cgctcttttc tcttaggttt
      481 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
      541 tgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa
      601 gccgttttac gtttggaact gacagaaccg caacgattga aggagccact cagccccaat
      661 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      721 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      781 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      841 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca
      901 ctatagaata ctcaagctat gcatccaacg cgttgggagc tctcccatat cgacctgcag
      961 gcggccgcac tagtgatatc ccgcggccat ggcggccggg agcatgcgac gtcgggccca
     1021 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     1081 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1141 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1201 gcgaatggaa attgtaaacg ttaatgggtt tctggagttt aatgagctaa gcacatacgt
     1261 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
     1321 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
     1381 atattcactc tcaatccaaa taatctgcaa tggcaattac cttatccgca acttctttac
     1441 ctatttccgc ccggatccgg gcaggttctc cggccgcttg ggtggagagg ctattcggct
     1501 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     1561 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     1621 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     1681 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     1741 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     1801 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     1861 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     1921 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     1981 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     2041 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     2101 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     2161 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     2221 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     2281 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     2341 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     2401 ccccgatcca acacttacgt ttgcaacgtc caagagcaaa tagaccacga acgccggaag
     2461 gttgccgcag cgtgtggatt gcgtctcaat tctctcttgc aggaatgcaa tgatgaatat
     2521 gatactgact atgaaacttt gagggaatac tgcctagcac cgtcacctca taacgtgcat
     2581 catgcatgcc ctgacaacat ggaacatcgc tatttttctg aagaattatg ctcgttggag
     2641 gatgtcgcgg caattgcagc tattgccaac atcgaactac ccctcacgca tgcattcatc
     2701 aatattattc atgcggggaa aggcaagatt aatccaactg gcaaatcatc cagcgtgatt
     2761 ggtaacttca gttccagcga cttgattcgt tttggtgcta cccacgtttt caataaggac
     2821 gagatggtgg agtaaagaag gagtgcgtcg aagcagatcg ttcaaacatt tggcaataaa
     2881 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     2941 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     3001 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     3061 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga attaattcca
     3121 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt
     3181 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     3241 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     3301 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     3361 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     3421 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     3481 tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat
     3541 ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata ataggaaatc
     3601 gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg aacgttgacg
     3661 atgtcgacgg atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
     3721 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     3781 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     3841 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     3901 tctgcgaggc tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga
     3961 aaccaagcca accaggggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
     4021 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
     4081 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta
     4141 aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca
     4201 ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc
     4261 tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca
     4321 gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc
     4381 taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc
     4441 gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc
     4501 ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg
     4561 aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct
     4621 gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct
     4681 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
     4741 tacccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga
     4801 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc
     4861 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc
     4921 atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg
     4981 cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag
     5041 caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt
     5101 ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc
     5161 catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc ggaataccaa
     5221 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
     5281 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
     5341 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
     5401 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
     5461 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
     5521 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
     5581 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac
     5641 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
     5701 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
     5761 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
     5821 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
     5881 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
     5941 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
     6001 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
     6061 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
     6121 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
     6181 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
     6241 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
     6301 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
     6361 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
     6421 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
     6481 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
     6541 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg
     6601 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac
     6661 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac
     6721 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg
     6781 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat
     6841 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag
     6901 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa
     6961 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc
     7021 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc
     7081 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc
     7141 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct
     7201 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc
     7261 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc
     7321 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc
     7381 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca
     7441 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg
     7501 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg
     7561 cgactcgaac ctacagttgt ttccttactg ggctttctca gccgggatgg cgctaagaag
     7621 ctattgccgc cgatcttcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7681 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8041 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     8101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8281 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8461 atcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8881 ttaaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtgcc aaaagccgcg
     8941 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
     9001 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
     9061 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
     9121 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
     9181 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
     9241 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
     9301 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
     9361 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
     9421 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
     9481 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
     9541 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     9601 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     9661 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     9721 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     9781 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     9841 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     9901 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     9961 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
    10021 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
    10081 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
    10141 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
    10201 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
    10261 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
    10321 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
    10381 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg
    10441 aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca
    10501 attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt
    10561 tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac
    10621 aactctgaga gcaactacga taatagttca tccaattacg acaatagtcg caacggaaat
    10681 cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc
    10741 aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa
    10801 ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata
    10861 aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac
    10921 tagcctgctc tctaataaaa tgttaggagc ttggctgcca tttttggggt gaggccgttc
    10981 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    11041 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgccagggc gcagccctgg
    11101 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    11161 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    11221 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    11281 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    11341 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    11401 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    11461 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    11521 aagttttccg cgaggtatcc acaacgccgg cggccggccg cggtgtctcg cacacggctt
    11581 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    11641 aaccagcccg gtgagcgtcg gaaaggg
//
