LOCUS       40924_4499        6749 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a hygromycin B resistance marker for transforming 
            Aspergillus species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CA.
  TITLE     Transformation of Aspergillus based on the hygromycin B resistance 
            marker from Escherichia coli.
  JOURNAL   Gene 1987;56:117-24.
  PUBMED    2824287
REFERENCE   2  (bases 1 to 6749)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "56"; pages: "117-24"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The trpC terminator region was corrected based on the updated 
            sequence record X02390.
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2129
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     intron          2178..2293
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2302..3318
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     terminator      3339..4101
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene (Punt et al., 1987)"
     primer_bind     complement(4124..4140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4614..4718
                     /label=AmpR promoter
     CDS             4719..5576
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5750..6338
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6626..6647
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6662..6692
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6700..6716
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6724..6740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
      121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
      181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
      241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
      301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
      361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
      421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
      481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
      541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
      601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
      661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
      721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
      781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
      841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
      901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
      961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     2281 cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     2341 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     2401 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     2461 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     2521 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     2581 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     2641 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     2701 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     2761 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     2821 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     2881 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     2941 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3001 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     3061 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     3121 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     3181 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     3241 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     3301 cgtccgaggg caaaggaata gagtagatgc cgaccgcggg atccacttaa cgttactgaa
     3361 atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga
     3421 gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag
     3481 agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt tttcgggttt
     3541 acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa cctagtaacg
     3601 ccttcaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac
     3661 gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc
     3721 tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg
     3781 atagcttgac tatgaaaatt ccgtcaccag ccctgggttc gcaaagataa ttgcatgttt
     3841 cttccttgaa ctctcaagcc tacaggacac acattcatcg taggtataaa cctcgaaatc
     3901 attcctacta agatggtata caatagtaac catggttgcc tagtgaatgc tccgtaacac
     3961 ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac
     4021 aggtacactt gtttagaggt aatccttctt tctagaagtc ctcgtgtact gtgtaagcgc
     4081 ccactccaca tctccactcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
     4141 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4201 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4261 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4321 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4381 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4441 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4501 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4561 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4681 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4741 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4801 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4861 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4921 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4981 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5041 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5101 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5161 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5221 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5281 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5341 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5401 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5461 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5521 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5581 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5881 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6361 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6421 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6481 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6541 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6601 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6661 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6721 acacaggaaa cagctatgac catgattac
//
