LOCUS       62056_3100        8270 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2129
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     intron          2178..2293
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2302..3318
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4100..4816
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4840..4856)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4858..5622
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene (Punt et al., 1987)"
     primer_bind     complement(5645..5661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6135..6239
                     /label=AmpR promoter
     CDS             6240..7097
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7271..7859
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8147..8168
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8183..8213
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8221..8237
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8245..8261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
      121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
      181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
      241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
      301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
      361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
      421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
      481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
      541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
      601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
      661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
      721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
      781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
      841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
      901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
      961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     2281 cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     2341 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     2401 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     2461 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     2521 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     2581 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     2641 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     2701 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     2761 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     2821 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     2881 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     2941 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3001 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     3061 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     3121 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     3181 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     3241 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     3301 cgtccgaggg caaaggaata gagtagatgc cgaccgcggc agaagtatgc aaagcatgcg
     3361 gagagacgga cggacgcaga gagaagggct gagtaataag cgccactgcg ccagacagct
     3421 ctggcggctc tgaggtgcag tggatgatta ttaatccggg accggccgcc cctccgcccc
     3481 gaagtggaaa ggctggtgtg cccctcgttg accaagaatc tattgcatca tcggagaata
     3541 tggagcttca tcgaatcacc ggcagtaagc gaaggagaat gtgaagccag gggtgtatag
     3601 ccgtcggcga aatagcatgc cattaaccta ggtacagaag tccaattgct tccgatctgg
     3661 taaaagattc acgagatagt accttctccg aagtaggtag agcgagtacc cggcgcgtaa
     3721 gctccctaat tggcccatcc ggcatctgta gggcgtccaa atatcgtgcc tctcctgctt
     3781 tgcccggtgt atgaaaccgg aaaggccgct caggagctgg ccagcggcgc agaccgggaa
     3841 cacaagctgg cagtcgaccc atccggtgct ctgcactcga cctgctgagg tccctcagtc
     3901 cctggtaggc agctttgccc cgtctgtccg cccggtgtgt cggcggggtt gacaaggtcg
     3961 ttgcgtcagt ccaacatttg ttgccatatt ttcctgctct ccccaccagc tgctcttttc
     4021 ttttctcttt cttttcccat cttcagtata ttcatcttcc catccaagaa cctttatcga
     4081 cctcgactct agaggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4141 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4201 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4261 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4321 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4381 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4441 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4501 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4561 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4621 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4681 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4741 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4801 ggacgagctg tacaagtaag aattcgatat caagcttatc gataccgtcg acctcgagac
     4861 ttaacgttac tgaaatcatc aaacagcttg acgaatctgg atataagatc gttggtgtcg
     4921 atgtcagctc cggagttgag acaaatggtg ttcaggatct cgataagata cgttcatttg
     4981 tccaagcagc aaagagtgcc ttctagtgat ttaatagctc catgtcaaca agaataaaac
     5041 gcgttttcgg gtttacctct tccagataca gctcatctgc aatgcattaa tgcattgact
     5101 gcaacctagt aacgccttnc aggctccggc gaagagaaga atagcttagc agagctattt
     5161 tcattttcgg gagacgagat caagcagatc aacggtcgtc aagagaccta cgagactgag
     5221 gaatccgctc ttggctccac gcgactatat atttgtctct aattgtactt tgacatgctc
     5281 ctcttcttta ctctgatagc ttgactatga aaattccgtc accagcncct gggttcgcaa
     5341 agataattgc atgtttcttc cttgaactct caagcctaca ggacacacat tcatcgtagg
     5401 tataaacctc gaaatcantt cctactaaga tggtatacaa tagtaaccat gcatggttgc
     5461 ctagtgaatg ctccgtaaca cccaatacgc cggccgaaac ttttttacaa ctctcctatg
     5521 agtcgtttac ccagaatgca caggtacact tgtttagagg taatccttct ttctagaagt
     5581 cctcgtgtac tgtgtaagcg cccactccac atctccactc gacctgcagg catgcaagct
     5641 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5701 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5761 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     5821 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     5881 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5941 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6001 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6061 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6241 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6361 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6421 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6481 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6541 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6601 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6781 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6841 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6901 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6961 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7081 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7141 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7381 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7861 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7921 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7981 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8041 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8101 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8161 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8221 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
//
