LOCUS       62056_3050        5697 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_100opt, complete sequence.
ACCESSION   MK606516
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(2960..3616)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3617..3719)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(4245..4790)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(4977..5687)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccactaa accgactgat ggtaatggtc ctactaaacc tacggatggt aatggcccga
      181 cgaagccgac tgacggtaat ggccctacta aaccaactga tggcaacggt ccaactaagc
      241 ctaccgacgg taacggtcca acgaaaccga ctgacggcaa tggcccaact aaacctacgg
      301 acggcaatgg tccaaccaaa cctaccgatg gtaacggccc aaccaaaccg accgacggca
      361 acggtccgac caagccgacc gacggtaatg gtccgaccaa accaaccgat ggcaacggcc
      421 ctaccaagcc gactgatggc aatggtccga cgaaacctac tgacggtaac ggcccgacga
      481 aaccgacgga cggcaacggc cctactaaac cgactgatgg taatggtcct actaaaccta
      541 cggatggtaa tggcccgacg aagccgactg acggtaatgg ccctactaaa ccaactgatg
      601 gcaacggtcc aactaagcct accgacggta acggtccaac gaaaccgact gacggcaatg
      661 gcccaactaa acctacggac ggcaatggtc caaccaaacc taccgatggt aacggcccaa
      721 ccaaaccgac cgacggcaac ggtccgacca agccgaccga cggtaatggt ccgaccaaac
      781 caaccgatgg taatggtccc actaaaccga ctgatggtaa tggtcctact aaacctacgg
      841 atggtaatgg cccgacgaag ccgactgacg gtaatggccc tactaaacca actgatggca
      901 acggtccaac taagcctacc gacggtaacg gtccaacgaa accgactgac ggcaatggcc
      961 caactaaacc tacggacggc aatggtccaa ccaaacctac cgatggtaac ggcccaacca
     1021 aaccgaccga cggcaacggt ccgaccaagc cgaccgacgg taatggtccg accaaaccaa
     1081 ccgatggcaa cggccctacc aagccgactg atggcaatgg tccgacgaaa cctactgacg
     1141 gtaacggccc gacgaaaccg acggacggca acggccctac taaaccgact gatggtaatg
     1201 gtcctactaa acctacggat ggtaatggcc cgacgaagcc gactgacggt aatggcccta
     1261 ctaaaccaac tgatggcaac ggtccaacta agcctaccga cggtaacggt ccaacgaaac
     1321 cgactgacgg caatggccca actaaaccta cggacggcaa tggtccaacc aaacctaccg
     1381 atggtaacgg cccaaccaaa ccgaccgacg gcaacggtcc gaccaagccg accgacggta
     1441 atggtccgac caaaccaacc gatggtaatg gtcccactaa accgactgat ggtaatggtc
     1501 ctactaaacc tacggatggt aatggcccga cgaagccgac tgacggtaat ggccctacta
     1561 aaccaactga tggcaacggt ccaactaagc ctaccgacgg taacggtcca acgaaaccga
     1621 ctgacggcaa tggcccaact aaacctacgg acggcaatgg tccaaccaaa cctaccgatg
     1681 gtaacggccc aaccaaaccg accgacggca acggtccgac caagccgacc gacggtaatg
     1741 gtccgaccaa accaaccgat ggcaacggcc ctaccaagcc gactgatggc aatggtccga
     1801 cgaaacctac tgacggtaac ggcccgacga aaccgacgga cggcaacggc cctactaaac
     1861 cgactgatgg taatggtcct actaaaccta cggatggtaa tggcccgacg aagccgactg
     1921 acggtaatgg ccctactaaa ccaactgatg gcaacggtcc aactaagcct accgacggta
     1981 acggtccaac gaaaccgact gacggcaatg gcccaactaa acctacggac ggcaatggtc
     2041 caaccaaacc taccgatggt aacggcccaa ccaaaccgac cgacggcaac ggtccgacca
     2101 agccgaccga cggtaatggt ccgaccaaac caaccgatgg taatggtccc actaaaccga
     2161 ctgatggtaa tggtcctact aaacctacgg atggtaatgg cccgacgaag ccgactgacg
     2221 gtaatggccc tactaaacca actgatggca acggtccaac taagcctacc gacggtaacg
     2281 gtccaacgaa accgactgac ggcaatggcc caactaaacc tacggacggc aatggtccaa
     2341 ccaaacctac cgatggtaac ggcccaacca aaccgaccga cggcaacggt ccgaccaagc
     2401 cgaccgacgg taatggtccg accaaaccaa ccgatggcaa cggccctacc aagccgactg
     2461 atggcaatgg tccgacgaaa cctactgacg gtaacggccc gacgaaaccg acggacggca
     2521 acggccctac taaaccgact gatggtaatg gtcctactaa acctacggat ggtaatggcc
     2581 cgacgaagcc gactgacggt aatggcccta ctaaaccaac tgatggcaac ggtccaacta
     2641 agcctaccga cggtaacggt ccaacgaaac cgactgacgg caatggccca actaaaccta
     2701 cggacggcaa tggtccaacc aaacctaccg atggtaacgg cccaaccaaa ccgaccgacg
     2761 gcaacggtcc gaccaagccg accgacggta atggtccgac caaaccaacc gatggtaatg
     2821 gtcccagaag agctaagtaa gtaagagctc gacccggtcg aatttgcttt cgaatttctg
     2881 ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat
     2941 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     3001 taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg
     3061 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     3121 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     3181 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     3241 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     3301 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     3361 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
     3421 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     3481 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     3541 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     3601 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     3661 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     3721 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     3781 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     3841 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     3901 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     3961 agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
     4021 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
     4081 gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     4141 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
     4201 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
     4261 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     4321 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
     4381 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     4441 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     4501 gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     4561 aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat
     4621 gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt
     4681 tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg
     4741 ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc
     4801 ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac
     4861 ctgaagtcag ccccatacga tataagttgt aattctcatg tttgacagca tgccatcgat
     4921 cgcgatcttg ctcaattgtt atcagctatg cgccgaccag aacaccttgc cgatcagcca
     4981 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg
     5041 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg
     5101 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc
     5161 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag
     5221 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct
     5281 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt
     5341 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac
     5401 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat
     5461 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca
     5521 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca
     5581 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt
     5641 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcatacg ttaaatc
//
