LOCUS       62056_3045        3267 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_10, complete sequence.
ACCESSION   MK606509
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(530..1186)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1187..1289)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1815..2360)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2547..3257)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccaccaa accgaccgac ggtaacgggc ccaccaaacc gaccgacggt aacgggccca
      181 ccaaaccgac cgacggtaac gggcccacca aaccgaccga cggtaacggg cccaccaaac
      241 cgaccgacgg taacgggccc accaaaccga ccgacggtaa cgggcccacc aaaccgaccg
      301 acggtaacgg gcccaccaaa ccgaccgacg gtaacgggcc caccaaaccg accgacggta
      361 acgggcccac caaaccgacc gacggtaacg ggcccagaag agctaagtaa gtaagagctc
      421 gacccggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
      481 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
      541 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      601 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      661 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      721 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
      781 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
      841 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      901 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
      961 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1021 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1081 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1141 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1201 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1261 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     1321 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     1381 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     1441 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     1501 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt
     1561 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     1621 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     1681 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     1741 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     1801 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     1861 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     1921 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     1981 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     2041 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     2101 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     2161 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     2221 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     2281 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     2341 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     2401 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     2461 aattctcatg tttgacagca tgccatcgat cgcgatcttg ctcaattgtt atcagctatg
     2521 cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata
     2581 actttcccca caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt
     2641 ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc
     2701 ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt
     2761 aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca
     2821 acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca
     2881 tcacctttgg taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa
     2941 acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg
     3001 tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca
     3061 agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct
     3121 gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc
     3181 tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt
     3241 ttcttttttg tgctcatacg ttaaatc
//
