LOCUS       40924_4449        3988 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAMGDlox-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Lu Y.
  TITLE     Establishing a novel L-ornithine self-sufficient cell factory with 
            enhanced production of fusarinine C through metabolic engineering in
            Aureobasidium melanogenum HN6.2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Lu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2018) Ocean University of China, College of Marine
            Life, 5 Yushan Road, Qingdao, Shangdong 266003, China
REFERENCE   3  (bases 1 to 3988)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3988)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2018) Ocean University of China, College of Marine Life, 5 
            Yushan Road, Qingdao, Shangdong 266003, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2259..2292)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(2302..2476)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2520..3086)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      complement(3087..3532)
                     /regulatory_class="promoter"
     protein_bind    complement(3533..3566)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3594..3610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcctgc aggtcgacta ccgttcgtat agcatacatt
     2281 atacgaagtt ataattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
     2341 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     2401 ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca
     2461 cattattatg gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactttat
     2521 ggacatggca tggacatgta caaagcctgt tcaccatcag aagcagtacc gtcgtacaaa
     2581 gcagtatcca aaccacacaa ggtgaaaccc attcttctgt aagcgtggat agctggagcg
     2641 ttgacgttgg taacctccaa ccacaagtga ccagcacctc tctctctagc gaactcagta
     2701 gccaaaccca tcaaagctct accgacaccg tgacctctgt gttctggagc gacctcgatg
     2761 tcttcaacgg tcaatcttct gttccaaccg gagtaagaga caacgacgaa accagccaaa
     2821 tcaccatcgt caccgtaagc gacgaaagtt ctggagtctg gatcaccgtc ctcaccagcg
     2881 tcggactcat cgtcagattc atcgtctggg aaaaccttgg tcaatggtgg atcgactgga
     2941 acttctctca aagtgaaacc gtcaccagta gcggtgactc tgaaaacagt atcagtggtg
     3001 aaggaaccgt ccaaagcctc gatagcttca gcatcacctg gaacagaagt tctgtatctg
     3061 taagcggtat cgtccaaagt ggtcattgtg actgaattgg atgtgttaga caggttgatg
     3121 tgaacgacag caacagagaa agacgcacag agttatatac aacagagctt gatgggttgt
     3181 tgtggtgtgt gaggggcttg aatctttccc caacagcttc cgacgctcgg catgaggggc
     3241 agctcaaaaa aaccgcgtca ccgacgtgct accgagatct tcgtgctctc tcgtgtttgc
     3301 ctatcaactg atatcaccaa tcatgagcca tctctatgta caaactgccc ttggatcacg
     3361 tgcttcactc gacatctgca ccatgtatcc ttcttcgaat tgctcatctt gatcacttta
     3421 ttattcaaga tgccttgcac cacacgacat gtctatcgat cggcccgtaa cgccccgtga
     3481 agttgccaga acgcctgcct gtcccgctgt ccgatggatg cttcaaggta atataacttc
     3541 gtatagcata cattatacga acggtaggat ccccgggtac cgagctcgaa ttcactggcc
     3601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3721 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     3781 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     3841 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3901 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3961 ttttcaccgt catcaccgaa acgcgcga
//
