LOCUS       40924_4439        5424 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAMEXlox-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Lu Y.
  TITLE     Establishing a novel L-ornithine self-sufficient cell factory with 
            enhanced production of fusarinine C through metabolic engineering in
            Aureobasidium melanogenum HN6.2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Lu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2018) Ocean University of China, College of Marine
            Life, 5 Yushan Road, Qingdao, Shangdong 266003, China
REFERENCE   3  (bases 1 to 5424)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2018) Ocean University of China, College of Marine Life, 5 
            Yushan Road, Qingdao, Shangdong 266003, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2717..3094
                     /regulatory_class="promoter"
     protein_bind    complement(3143..3176)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3187..3361)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3405..3971)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     protein_bind    complement(4418..4451)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5030..5046)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgccccg gggctaaaaa ccccaacttc ggaaaggggt
     2281 gtatttatta gataaaaaac caacgccttt cggggctcct tggtgattca taataactaa
     2341 acgaatcgca tggccttgcg ccggcgatgg ttcattcaaa tttctgccct atcaactttc
     2401 gatggtagga tagtggccta ccatggtatc aacgggtaac ggggaattag ggttctattc
     2461 cggagaggga gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta
     2521 cccaatcccg acacggggag gtagtgacaa taaatactga tacagggctc ttttgggtct
     2581 tgtaattgga atgagtacaa tttaaatccc ttaacgagga acaattggag ggcaagtctg
     2641 gtgccagcag ccgcggtaat tccagctcca atagcgtata ttaaagttgt tgcagttaaa
     2701 aagctcgtag ttgaaccgac agttggctca tcatccgtta catcatcttt ttttctgctg
     2761 gctccgctcg gtgggctccc aacgaagcag caaaaaaagt gagagagaaa aactagcttg
     2821 gcggggcatc cgaagctaga ccctttggct cgcttagtca gtgcgccctc tcacacacac
     2881 tcaaaaaggc cacccctccc gcaccctctt ctcatcaccg tcttcatacc acggttcgtc
     2941 aagcaatcgt atctggtaag ctttgacctc ttgagcgggc tccactttgc tatttcttgg
     3001 atctgctcat tcttttcttc cttcctctct ttctaacctc tcttcagaaa gttcaaccgt
     3061 acttcactca atcttccata catcaccgtc aaacactagt cttaagatgc atacgcgtga
     3121 gctcctgcag ggtaccgtcg actaccgttc gtatagcata cattatacga agttattaat
     3181 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     3241 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     3301 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     3361 actcgagctt gtcgatcgac agatcccggt cggcatctac tttatggaca tggcatggac
     3421 atgtacaaag cctgttcacc atcagaagca gtaccgtcgt acaaagcagt atccaaacca
     3481 cacaaggtga aacccattct tctgtaagcg tggatagctg gagcgttgac gttggtaacc
     3541 tccaaccaca agtgaccagc acctctctct ctagcgaact cagtagccaa acccatcaaa
     3601 gctctaccga caccgtgacc tctgtgttct ggagcgacct cgatgtcttc aacggtcaat
     3661 cttctgttcc aaccggagta agagacaacg acgaaaccag ccaaatcacc atcgtcaccg
     3721 taagcgacga aagttctgga gtctggatca ccgtcctcac cagcgtcgga ctcatcgtca
     3781 gattcatcgt ctgggaaaac cttggtcaat ggtggatcga ctggaacttc tctcaaagtg
     3841 aaaccgtcac cagtagcggt gactctgaaa acagtatcag tggtgaagga accgtccaaa
     3901 gcctcgatag cttcagcatc acctggaaca gaagttctgt atctgtaagc ggtatcgtcc
     3961 aaagtggtca ttgtgactga attggatgtg ttagacaggt tgatgtgaac gacagcaaca
     4021 gagaaagacg cacagagtta tatacaacag agcttgatgg gttgttgtgg tgtgtgaggg
     4081 gcttgaatct ttccccaaca gcttccgacg ctcggcatga ggggcagctc aaaaaaaccg
     4141 cgtcaccgac gtgctaccga gatcttcgtg ctctctcgtg tttgcctatc aactgatatc
     4201 accaatcatg agccatctct atgtacaaac tgcccttgga tcacgtgctt cactcgacat
     4261 ctgcaccatg tatccttctt cgaattgctc atcttgatca ctttattatt caagatgcct
     4321 tgcaccacac gacatgtcta tcgatcggcc cgtaacgccc cgtgaagttg ccagaacgcc
     4381 tgcctgtccc gctgtccgat ggatgcatga aggtaatata acttcgtata gcatacatta
     4441 tacgaacggt aggatcccca acagggattg ccctagtaac ggcgagtgaa gcggcaacag
     4501 ctcaaatttg aaagctggcc ttcgggtccg cattgtaatt tgtagaggat gctttgggtg
     4561 aaacgccagt ctaagttcct tggaacagga cgtcatagag ggtgagaatc ccgtatgtga
     4621 ctggaaatgt taacctatgt aaagctcctt cgacgagtcg agttgtttgg gaatgcagct
     4681 ctaaatggga ggtaaatttc ttctaaagct aaatattggc gagagaccga tagcgcacaa
     4741 gtagagtgat cgaaagatga aaagcacttt ggtaagagag ttaaaaagca cgtgaaattg
     4801 ttgaaaggga agcgcttgca atcagacttg tttaaactgt tcggccggtc ttctgaccgg
     4861 tttactcagt ttggacaggc cagcatcagt ttcggcggcc ggataaaggc tttgggaatg
     4921 tggccttcac ttcggtgaag gtgttgtagc ccagggtgta atacggccag ccgggactga
     4981 ggtccgcgct tcggctagga tgctggcgta atggttggaa ttccccggga ctggccgtcg
     5041 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5101 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5161 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     5221 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5281 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5401 caccgtcatc accgaaacgc gcga
//
