LOCUS       40924_4404        5256 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Saccharomyces cerevisiae vector pAM634, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 5256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian Academy 
            of Science, Moscow 119071, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    45..78
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_recomb     complement(157..190)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(157..190)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(215..656)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     intron          790..840
     CDS             844..1827
                     /label=Cre
                     /note="site-specific recombinase"
     rep_origin      complement(1903..2491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2665..3522)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3523..3627)
                     /label=AmpR promoter
     promoter        3733..4135
                     /label=LEU2 promoter
     CDS             4136..5227
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
ORIGIN
        1 gtacaccttg tacgcagcgt cgaagccggt cgagaaccct taatataact tcgtataatg
       61 tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc ggccgccagc
      121 tgaagcttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
      181 acgaagttat taggtctaga atccggggtt ttttctcctt gacgttaaag tatagaggta
      241 tattaacaat tttttgttga tacttttatt acatttgaat aagaagtaat acaaaccgaa
      301 aatgttgaaa gtattagtta aagtggttat gcagtttttg catttatata tctgttaata
      361 gatcaaaaat catcgcttcg ctgattaatt accccagaaa taaggctaaa aaactaatcg
      421 cattatcatc ctatggttgt taatttgatt cgttcatttg aaggtttgtg gggccaggtt
      481 actgccaatt tttcctcttc ataaccataa aagctagtat tgtagaatct ttattgttcg
      541 gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc gaccggtgaa gacgaggacg
      601 cacggaggag agtcttcctt cggagggctg tcacccgctc ggcggcttct aatccgtact
      661 agaggctgcc gctaagtgta cccaaccgtc cccacacggt ccatctataa atactgctgc
      721 cagtgcacgg tggtgacatc aatccaaagt tactacaaga caaatagaca agtcaagtta
      781 gataaaatgg taagttgcca atgccagagg cagcatgtgc aagtgttcta acctttctag
      841 tcaatcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc
      901 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca
      961 tggtgcaagt tgaataaccg ggaatggttt cccgcagaac ctgaagatgt tcgcggttat
     1021 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag
     1081 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca
     1141 ctggttatgc ggcggatcag aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct
     1201 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc
     1261 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata
     1321 gccgaaattg ccaggatcag ggttaaagac atctcacgta ctgacggtgg gagaatgtta
     1381 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc
     1441 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg
     1501 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc
     1561 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc
     1621 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga
     1681 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc
     1741 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca
     1801 atggtgcgcc tgctggaaga tggcgattag ccattaacgc gccatgtgag caaaaggcca
     1861 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1921 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1981 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2041 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2101 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2161 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2221 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2281 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2341 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2401 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2461 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2521 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2581 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2641 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2701 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2761 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2821 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2881 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2941 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3001 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3061 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3121 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3181 gccatccgta agatgctttt ctgtgactgg tgagtattca accaagtcat tctgagaata
     3241 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3301 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3361 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3421 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3481 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3541 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3601 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3661 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3721 tcttcaagaa ttaactgtgg gaatactcag gtatcgtaag atgcaagagt tcgaatctct
     3781 tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct atcgcacaga
     3841 atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct
     3901 ttttcaactg aaaaattggg agaaaaagga aaggtgagag gccggaaccg gcttttcata
     3961 tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat
     4021 tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttat tctaactttt
     4081 cttacctttt acatttcagc aatatatata tatatttcaa ggatatacca ttctaatgtc
     4141 tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa tcacagccga
     4201 agccattaag gttcttaaag ctatttctga tgttcgttcc aatgtcaagt tcgatttcga
     4261 aaatcattta attggtggtg ctgctatcga tgctacaggt gtcccacttc cagatgaggc
     4321 gctggaagcc tccaagaagg ttgatgccgt tttgttaggt gctgtgggtg gtcctaaatg
     4381 gggtacaggt agtgttagac ctgaacaagg tttactaaaa atccgtaaag aacttcaatt
     4441 gtacgccaac ttaagaccat gtaactttgc atccgactct cttttagact tatctccaat
     4501 caagccacaa tttgctaaag gtactgactt cgttgttgtc agagaattag tgggaggtat
     4561 ttactttggt aagagaaagg aagacgatgg tgatggtgtc gcttgggata gtgaacaata
     4621 caccgttcca gaagtgcaaa gaatcacaag aatggccgct ttcatggccc tacaacatga
     4681 gccaccattg cctatttggt ccttggataa agctaatgtt ttggcctctt caagattatg
     4741 gagaaaaact gtggaggaaa ccatcaagaa cgaatttcct acattgaagg ttcaacatca
     4801 attgattgat tctgccgcca tgatcctagt taagaaccca acccacctaa atggtattat
     4861 catcaccagc aatatgtttg gtgacatcat ctccgatgaa gcctccgtta tcccaggttc
     4921 cttgggtttg ttgccatctg cgtccttggc ctctttgcca gacaagaaca ccgcatttgg
     4981 tttgtacgaa ccatgccacg gttctgctcc agatttgcca aagaataagg ttgaccctat
     5041 cgccactatc ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc ctgaagaagg
     5101 taaggccatt gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa ctggtgattt
     5161 aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat
     5221 ccttgcttaa aaagattctc tttttttatg atattt
//
