LOCUS       40924_4399        5014 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Hansenula polymorpha vector pAM631, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 5014)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian Academy 
            of Science, Moscow 119071, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..49
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          551..601
     CDS             605..1588
                     /label=Cre
                     /note="site-specific recombinase"
     rep_origin      complement(1664..2252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2426..3283)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3284..3388)
                     /label=AmpR promoter
     CDS             3720..4811
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     misc_recomb     complement(4889..4922)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(4889..4922)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 tcgacaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctagaa
       61 aacccactcg ctttagccgt cgcgtaaaac ttgtgaatct ggcaactgag ggggttctgc
      121 agccgcaacc gaacttgtcg ctccgaggac gcggctggat ggtggcatgt gaggctctgt
      181 ttgctggcgt agcctacaac gtgaccttac ctaaccggac ggcgctaccc actgctgtct
      241 gtgcctgcta ccagaaaatc accagagcag cagagggccg atgtggcaac tggtggggtg
      301 tcggacaggc tgtttctcca cagtgcaaat gcgggtgaac cggccagaaa gtaaattctt
      361 atgctaccgt gcagcgactc cgacatcccc agtttttgcc ctacttgatc acagatgggg
      421 tcagcgctgc cgctaagtgt acccaaccgt ccccacacgg tccatctata aatactgctg
      481 ccagtgcacg gtggtgacat caatccaaag ttactacaag acaaatagac aagtcaagtt
      541 agataaaatg gtaagttgcc aatgccagag gcagcatgtg caagtgttct aacctttcta
      601 gtcaatcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg
      661 ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc
      721 atggtgcaag ttgaataacc gggaatggtt tcccgcagaa cctgaagatg ttcgcggtta
      781 tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca
      841 gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc
      901 actggttatg cggcggatca gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc
      961 tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg
     1021 ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat
     1081 agccgaaatt gccaggatca gggttaaaga catctcacgt actgacggtg ggagaatgtt
     1141 aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag
     1201 cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc
     1261 gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag
     1321 ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg
     1381 cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg
     1441 agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg
     1501 ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc
     1561 aatggtgcgc ctgctggaag atggcgatta gccattaacg cgccatgtga gcaaaaggcc
     1621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2101 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2341 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2401 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2461 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2521 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2581 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2641 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2701 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2761 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2821 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2881 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2941 tgccatccgt aagatgcttt tctgtgactg gtgagtattc aaccaagtca ttctgagaat
     3001 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3061 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3121 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3181 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3241 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3301 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3361 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     3421 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     3481 gtcttcaaga attaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
     3541 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
     3601 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
     3661 tttttcaact gattacattt cagcaatata tatatatatt tcaaggatat accattctaa
     3721 tgtctgcccc taagaagatc gtcgttttgc caggtgacca cgttggtcaa gaaatcacag
     3781 ccgaagccat taaggttctt aaagctattt ctgatgttcg ttccaatgtc aagttcgatt
     3841 tcgaaaatca tttaattggt ggtgctgcta tcgatgctac aggtgtccca cttccagatg
     3901 aggcgctgga agcctccaag aaggttgatg ccgttttgtt aggtgctgtg ggtggtccta
     3961 aatggggtac aggtagtgtt agacctgaac aaggtttact aaaaatccgt aaagaacttc
     4021 aattgtacgc caacttaaga ccatgtaact ttgcatccga ctctctttta gacttatctc
     4081 caatcaagcc acaatttgct aaaggtactg acttcgttgt tgtcagagaa ttagtgggag
     4141 gtatttactt tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg gatagtgaac
     4201 aatacaccgt tccagaagtg caaagaatca caagaatggc cgctttcatg gccctacaac
     4261 atgagccacc attgcctatt tggtccttgg ataaagctaa tgttttggcc tcttcaagat
     4321 tatggagaaa aactgtggag gaaaccatca agaacgaatt tcctacattg aaggttcaac
     4381 atcaattgat tgattctgcc gccatgatcc tagttaagaa cccaacccac ctaaatggta
     4441 ttatcatcac cagcaatatg tttggtgaca tcatctccga tgaagcctcc gttatcccag
     4501 gttccttggg tttgttgcca tctgcgtcct tggcctcttt gccagacaag aacaccgcat
     4561 ttggtttgta cgaaccatgc cacggttctg ctccagattt gccaaagaat aaggtcaacc
     4621 ctatcgccac tatcttgtct gctgcaatga tgttgaaatt gtcattgaac ttgcctgaag
     4681 aaggtaaggc cattgaagat gcagttaaaa aggttttgga tgcaggtatc agaactggtg
     4741 atttaggtgg ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa gaagttaaga
     4801 aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacgtacac cttgtacgca
     4861 gcgtcgaagc cggtcgagaa cccttaatat aacttcgtat aatgtatgct atacgaagtt
     4921 attaggtgat atcagatcca ctagtggcct atgcggccgc cagctgaagc ttcgaattcg
     4981 agctcccggg taccatggca tgcatcgata gatc
//
