LOCUS       40924_4394        4985 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Hansenula polymorpha vector pAM619, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 4985)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4985)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian Academy 
            of Science, Moscow 119071, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    45..78
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_recomb     157..190
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(157..190)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          692..742
     CDS             746..1729
                     /label=Cre
                     /note="site-specific recombinase"
     rep_origin      complement(1805..2393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2567..3424)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3425..3529)
                     /label=AmpR promoter
     CDS             3861..4952
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
ORIGIN
        1 gtacaccttg tacgcagcgt cgaagccggt cgagaaccct taatataact tcgtataatg
       61 tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc ggccgccagc
      121 tgaagcttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
      181 acgaagttat taggtctaga aaacccactc gctttagccg tcgcgtaaaa cttgtgaatc
      241 tggcaactga gggggttctg cagccgcaac cgaacttgtc gctccgagga cgcggctgga
      301 tggtggcatg tgaggctctg tttgctggcg tagcctacaa cgtgacctta cctaaccgga
      361 cggcgctacc cactgctgtc tgtgcctgct accagaaaat caccagagca gcagagggcc
      421 gatgtggcaa ctggtggggt gtcggacagg ctgtttctcc acagtgcaaa tgcgggtgaa
      481 ccggccagaa agtaaattct tatgctaccg tgcagcgact ccgacatccc cagtttttgc
      541 cctacttgat cacagatggg gtcagcgctg ccgctaagtg tacccaaccg tccccacacg
      601 gtccatctat aaatactgct gccagtgcac ggtggtgaca tcaatccaaa gttactacaa
      661 gacaaataga caagtcaagt tagataaaat ggtaagttgc caatgccaga ggcagcatgt
      721 gcaagtgttc taacctttct agtcaatcga tgcaacgagt gatgaggttc gcaagaacct
      781 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
      841 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cgggaatggt ttcccgcaga
      901 acctgaagat gttcgcggtt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
      961 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
     1021 aagtgacagc aatgctgttt cactggttat gcggcggatc agaaaagaaa acgttgatgc
     1081 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     1141 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     1201 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag acatctcacg
     1261 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     1321 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     1381 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     1441 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     1501 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     1561 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     1621 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     1681 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt agccattaac
     1741 gcgccatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2221 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtatt
     3121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3541 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3601 ggcgtatcac gaggcccttt cgtcttcaag aattaactgt gggaatactc aggtatcgta
     3661 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca
     3721 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
     3781 aggtcgcctg acgcatatac ctttttcaac tgattacatt tcagcaatat atatatatat
     3841 ttcaaggata taccattcta atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc
     3901 acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt tctgatgttc
     3961 gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct atcgatgcta
     4021 caggtgtccc acttccagat gaggcgctgg aagcctccaa gaaggttgat gccgttttgt
     4081 taggtgctgt gggtggtcct aaatggggta caggtagtgt tagacctgaa caaggtttac
     4141 taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac tttgcatccg
     4201 actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact gacttcgttg
     4261 ttgtcagaga attagtggga ggtatttact ttggtaagag aaaggaagac gatggtgatg
     4321 gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc acaagaatgg
     4381 ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg gataaagcta
     4441 atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc aagaacgaat
     4501 ttcctacatt gaaggttcaa catcaattga ttgattctgc cgccatgatc ctagttaaga
     4561 acccaaccca cctaaatggt attatcatca ccagcaatat gtttggtgac atcatctccg
     4621 atgaagcctc cgttatccca ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt
     4681 tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccacggttct gctccagatt
     4741 tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat
     4801 tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg
     4861 atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa gtcggtgatg
     4921 ctgtcgccga agaagttaag aaaatccttg cttaaaaaga ttctcttttt ttatgatatt
     4981 tgtac
//
