LOCUS       40924_4384        3323 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAM3G, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Kang Y, Norris MH, Hoang TT.
  TITLE     Knock-out and pull-out recombineering protocols for natural 
            transformable Burkholderia thailandensis and Burkholderia 
            pseudomallei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Kang Y, Norris MH, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2009) Molecular Biosciences and Biological 
            Engineering, University of Hawaii at Manoa, 2538 McCarthy Mall, 
            Snyder Hall 207, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 3323)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-OCT-2009) Molecular Biosciences and Biological Engineering, 
            University of Hawaii at Manoa, 2538 McCarthy Mall, Snyder Hall 207, 
            Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(12..363)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             377..1207
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     1371..1387
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1388..1444
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1457..1473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1481..1497)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1505..1535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1550..1571)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            1955..2063
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2131..2719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2803..3243)
                     /codon_start=1
                     /gene="gat"
                     /product="glyphosate acetyl transferase"
                     /label=gat
                     /note="confers resistance to glyphosate"
                     /protein_id="ADO63834.1"
                     /translation="MIEVKPINAEDTYDLRHRVLRPNQPIEACMFESDLTRSAFHLGGF
                     YGGKLISVASFHQAEHSELQGKKQYQLRGVATLEGYREQKAGSSLVKHAEEILRKRGAD
                     MIWCNARTSASGYYRKLGFSEQGEVFDTPPVGPHILMYKRIT"
     gene            complement(2803..3243)
                     /gene="gat"
                     /label=gat
     regulatory      3282..3323
                     /note="PCS12; rpsL promoter from Burkholderia cenocepacia"
                     /regulatory_class="promoter"
ORIGIN
        1 agcgctagat ctccgcctcg ataccctgat tactcgcttc ctgcgccctc tcaggcggcg
       61 ataggggact ggtaaaacgg ggattgccca gacgcctccc ccgccccttc aggggcacaa
      121 atgcggcccc aacggggcca cgtagtggtg cgttttttgc gtttccaccc ttttcttcct
      181 tttccctttt aaacctttta ggacgtctac aggccacgta atccgtggcc tgtagagttt
      241 aaaaagggac ggatttgttg ccattaaggg acggatttgt tgttaagaag ggacggattt
      301 gttgttgtaa agggacggat ttgttgtatt gtgggacgca gatacagtgt ccccttatac
      361 acaaggaatg tcgaacgtgg cctcaccccc aatggtttac aaaagcaatg ccctggtcga
      421 ggccgcgtat cgcctcagtg ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt
      481 gaagaggagc gagcctgtca ccgatgaagt gatgtattca gtgacggcgg aggacatagc
      541 gacgatggcg ggtgtcccta tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg
      601 cctgaaacgg cgggaagtcc ggttaaccca agagcccaat ggcaagggga aaagaccgag
      661 tgtgatgatt accggctggg tgcaaacaat catctaccgg gagggtgagg gccgtgtaga
      721 actcaggttc accaaagaca tgctgccgta cctgacggaa ctcaccaaac agttcaccaa
      781 atacgccttg gctgacgtgg ccaagatgga cagcacccac gcgatcaggc tttacgagct
      841 gctcatgcaa tgggacagca tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg
      901 gtttcaactg gaaggccggt atccctcgat caaggacttc aagttgcgag tgcttgatcc
      961 agccgtgacg cagatcaacg agcacagccc gctacaggtg gagtgggcgc agcgaaagac
     1021 cgggcgcaag gtcacacatc tgttgttcag ttttggaccg aagaagcccg ccaaggcggt
     1081 gggtaaggcc ccagcgaagc gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca
     1141 ggctcgccct ggtgagacat gggaagcggc ccgcgctcga ctaacccaga tgccgctgga
     1201 tctggcctag aggccgtggc caccacggcc cggcctgcct ttcaggctgc gcaactgttg
     1261 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     1321 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     1381 ggccagtgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
     1441 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1801 tggggattcc ttaaggtata ctttccgctg cataaccctg cttcggggtc attatagcga
     1861 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     1921 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     1981 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     2041 atcctgctct gcgaggctgg ccgataagct catgtgagca aaaggccagc aaaaggccag
     2101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2761 gaacgaaaac tcacgttaag ggattttggt catgatctcg agttaggtga tccgcttgta
     2821 catcaggatg tggggcccca cgggcggggt gtcgaagacc tccccctgct cgctgaaccc
     2881 cagcttccgg tagtaccccg aggccgaggt ccgggcgttg caccagatca tgtcggcccc
     2941 ccgcttccgc aggatctctt cggcgtgctt gaccagcgac gacccggcct tctgctcccg
     3001 gtagccctcc agggtggcca caccgcgcag ctggtactgc ttcttcccct gaagctccga
     3061 gtgctcggcc tggtggaacg aagcgaccga aatcagcttg cccccgtaga acccgcccag
     3121 gtggaaggcg ctccgcgtga gatcgctttc gaacatgcac gcctcgatcg gctggttggg
     3181 ccggaggacc cgatgccgca ggtcgtaggt gtcctcggcg ttgatgggct tgacctcgat
     3241 catatgcttg tcctttcgaa gctcgatccc atggtcccgg aacccagcat gatattccgg
     3301 aaataccaac taagtcaacg gct
//
