LOCUS       40924_4374       10577 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAM1956, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Videau P, Callahan SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Microbiology, University of Hawaii, 2538 
            McCarthy Mall, Snyder Hall 201, Honolulu, HI 96822, USA
REFERENCE   2  (bases 1 to 10577)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10577)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-APR-2014) Microbiology, University of Hawaii, 2538 McCarthy 
            Mall, Snyder Hall 201, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Gene Construction Kit v. 3.5
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6744..7457
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      7584..8172
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8358..8498)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(9048..9836)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 atctagactt atatagacac taatatagac aatagtttat actgctatct atacaagtat
       61 agacattatc taatcatggc agacaaaact ctagccactt ttcgtattga ctccgaagaa
      121 tgggagtctt ttaaaaacct tgctagttct gaaagttcca acgcctcagc actgttaaca
      181 gaatttgttc gttggtattt ggcaggtaac aggtttaata ctcccacttc tcacactccc
      241 acccatctag acacatccct cgaacagcgt atagacaata ttgaacaacg tctagataaa
      301 gtcacaacta ataatctaga caatatagat gaatttatag acaagcgtat agaagataat
      361 ctagcaacac gtctagacaa acttcaatcg caactggagg aactgcgggg aaaatcgaaa
      421 gcccggtagt tcaggcagaa ggacaagcta ccgggcaaga cagaaagaat atagacaata
      481 gtatagacaa tctagacaaa ttggaggcaa cccgcgatcg caccctcaat aagctaaaaa
      541 tgggtaggca gtcagccgcc gggaaagcca tcgacgcgtt tatcaaagag ttgctttctt
      601 caggagacaa cataagctga agttatcaaa attctgtcct tacgtcgaaa gcctgatttt
      661 accgtgcaac gattgataag cttggctaaa ctagcactgg ctttcaacag aaagcatacg
      721 aagaatcaat agatatagcc accaattcca caaaatgcag ataacgtgta gagtattgga
      781 atgcttaatc tgtaagggtt atgaaggtta acggcaacgg acgagccaaa atactcacct
      841 ccgacgaact caggcgactg tttagcgacg gattcaccac accgcgcgat cgcgttttgt
      901 ttggcatctg tctattcacc ggttgccgcg ttagtgaagc tctagcactc caaacaacgg
      961 acattaaagg cgaaacacta acctttagga agtctaccac caaagggaaa ctcaaaaccc
     1021 gcgtggttga catccagcca ggactagccg cactcatggc tgactatcac cccaaaccgg
     1081 gaaccctgtt ccctggcatg aggggagtca gcgataggct cacgcgatac gcggcggata
     1141 aaatcttgcg cgatgcagcc aaaagaatcg ggctagaagg catcagtacc cacagtttcc
     1201 gccgtactgc cctcaaccaa atgtctagcg ccggtatccc gttgcgacac attcaagaga
     1261 tatccggtca caatgacctt ggcacactgc aacgctatct tgaagttaca cccgaacagc
     1321 gacgcaaagc tgtatccgtg attggcttct aatgtacgcc aacgctgttt agacccctat
     1381 gggtgctaaa aaaagacgca gcctaaacac acgctctaca cttgaggata cttttaaagt
     1441 atccatcggt tctagaactc tgcacacgtt ccggactttg gaaacgttat acctttccct
     1501 gtgttgcaga atgctgcaat atttcttcga caagttaact tgtgactggt ttaatatttt
     1561 ctcaaattgc cccaaaacaa cacgcctaaa tccttagacg tttctgtgga aacctattag
     1621 gtttttatcg ccgttgtttt agtggtaaac ccaaagggtt tgtatattct tgtatgaagt
     1681 tcgactctga gggttaagaa gaatggctcg ccgaattttt tacaagtgga aaccgattaa
     1741 aggttaaggg tcaatcggga cgatgaatat tttctaattg tgaccttctc catctaataa
     1801 gctttctttg gggttaaggt cgaagaaagt actacgcatg atctgcatac gatctctatt
     1861 gccaaaaagc cgcgacccta taggctctcg gtcatgctgc actagttcgt gtcgatcact
     1921 atactggttg ccgcagcatt tcacgctaaa aaaaaattct taaaaatgtc cttcatatct
     1981 cgccagagtg gcaacctatt acaaaacggt tgcctacccg accggctcga ttttcgctga
     2041 agtggcactg tgacagtttg aaatggtact tccgccgtgc tgctgacatc gttgttaggg
     2101 tgaattgttc gcggtagatg ttgcaccgat tcatgaacac cttgtcaccc actttgaata
     2161 atcgaccgtc aaattcagtc gcgtcaattt ggtaagtgtt gggctgtctc tttttggctc
     2221 caggggcaat gccatcagaa aacacaaccg cgtcacccat aacttgataa ccgatatcag
     2281 ttttggttcc agtgaaagcc caaaattcag acgcgtcatt attccgagcg tgccggagtt
     2341 gattgtactc aattttggct tggcaaagtt gacggcgatt catgcccagc tgcttttgat
     2401 gtcgtcgcac tgtgcgcttg tgaataccca actcacagct gacagctttt tgagatgtac
     2461 catagtggat gaaacttttt gagacgaata tccgcgacga actaatgtga agtacacaag
     2521 gtacttcccc ctctggcgat ttaagagagg attgccttgt gtccttcact agctcgttcg
     2581 ggtgtggcgc tccaaaaagt tttctgtact ctggtttaag ttgtctgttg gccgcatagc
     2641 ggctcttttg ttgaaagctt tgtgtgacta tgccagtggt cagtgagcgt aaatcgctta
     2701 acacttggac taaaggcact actgcaacat caccccatct ttttaaattt aggttgtaac
     2761 aaacttgaaa cataccgccc aagtagacgg ttatcattcc tgctttaatt ttgtagcggc
     2821 ggaatgctcc tatttttttt ccatcctgta accaacggta aacagactta tcactacaat
     2881 ctaagaacgt ctgtactaca ggcaatggca atgttaaatg accagaccca tccttatcaa
     2941 gcgctcgaca caaataccac aaccgcgcac aaggttctcg accaatgcga gtgtgtaccc
     3001 tgaccgtgta agtgccaaga attatttcag tttgtagttc ccttgtaagc agggttagtg
     3061 atacatttgt atttaagctt tctgggctga tcatttggaa atgtctcagt ccagtaccta
     3121 ttgaatgtta tttgcttaac ctgaagctaa ataaaacttg ttaactacac ccattaattg
     3181 ataaattcaa agcacgtttt ttctgtttgg tgtttggtgt ggtaacaatt ctgtgtatgt
     3241 gtgttttatt tagcttcggt taagtagcat aacaaccccc aagcactgaa ctttttttaa
     3301 taggtaattt aaactttgcc tatcggcaaa attttcaatc aattgtacgc caaagtgttg
     3361 catgatcaac gtttgactta tttttgtatt tactaaatac tgaatttcgc cgtgacgctt
     3421 tttacagatg gaaattcacg gcaaaatgtt ttttgctaac tttgctatgt aaaacaagaa
     3481 acttggcact cggttattac taaataaact ggtaaaaaat aaccattaga accaaaaaga
     3541 acgaaaacca gtacaccctt gccagttttc aagcttttgc tatgacgact ctaataatcg
     3601 ggtttaacac cattccgctt tgagaaaatt atccttgtac agcaagtaac agtcaatgct
     3661 aaaccgcacc gctacaaatc cttaagtttt tccagtagcg atttaccttc ttggtaacgc
     3721 ccgccttgat agcccaaaat ttctttaatc accttacttt ctgaaaaacc cgcttccaga
     3781 caggctttta ccacttttgc tagggtttca tctcttggtt ctgggaggga tgaaacgggc
     3841 tgtaatgctt gttctgaggt cggttgagcc gtttggagtg gctgaaaact ggttacagac
     3901 tgtaaccggg gcataaccat tttgtaactg cttacatctg gtaactgaca cggcatatca
     3961 tccaccatgc agcgatattt ccccgacttt aaccactcca caagggcaag gtcttttaag
     4021 gacttggcgt ggctaactgc aaacttaccc aggcgtaaca tcctaaaaca cttacggaca
     4081 ccgccttcac cctcgatacc taaggtcttg acattatcat cttgagtcag cccaataaca
     4141 aaacgcttgg gcttgcggcc gcgcctggcg tgtttgatga gccattcggt tgctatctcg
     4201 acttcatctc tcagcagtgg cagttcttca gcaattaaaa cgctttcttt tcctgctagt
     4261 gccttatccc cagactcacc ccgtagctca atccggcgct gcaattcctc caggtcagca
     4321 gccatgcccg actgtatagc ctcaaagtca ccacggcggc caatgacatt taaccccgtc
     4381 cactcgtccg gtgcagcgtc agcgtcatag actgtcacct cacccccgac ttgataagca
     4441 agccattggg ctatggtgct tttgccagtt cccgtatccc caactattaa acagtgctta
     4501 ccagacagag cttgcatcaa gtcggtgatg attccctctg gttcgaccgc aagggtgacg
     4561 gcggtagtgt caatgatagc cgcgccgtaa gtgccagcat agggcaattg gtcgtaaact
     4621 ttgaccaagt tgtatacaga ctgtctacac cacttcacca ctgttaacgc tgtttgcaaa
     4681 gcgtaagacg tggcatcaaa taaaaatatg ctggcactaa aagttaatcg ccccaatccc
     4741 cacagtaaaa acctgcctag ctgttgacga ctaggcaagt gcatttcaat ccagtcattt
     4801 gccataaatc accccgtctt taaagccttg cagttgagcg cgacaggtat ttaactgtgc
     4861 ttgtaactct gtttgctggt tttgatacca cagactgacg gcggcggccg ccagtcctaa
     4921 aaatagaaac tggcgatcgc tcattattga cttactccct gttgattagc gtggtagtga
     4981 gtcatagccg cattgaccgc ttcttgggct tggggtgttc tgccaagatt gggttttgta
     5041 gggtcatcgt tggctacgac taaggacgct tgttcggcta tcgcttgcgg gacaccaact
     5101 ttagttaact ctgtcaagga tacttggtaa agtcgctcgt tcattagccg attctccggt
     5161 acataaaact gttgctggca gtcccttcat tggcgacgag ttcttcagcc ggagtatcag
     5221 cgataatgtc agcccagccg gtgacattat tattaataat gttttgttcg gcaattgcac
     5281 ccaagccagg acgcgccgtt tcaaactcag agatgacttg ctgctctttc tcggtgagtg
     5341 gtctatctgt catgataatt atgtccttca ttatgtaggc gattccagtg ggtgtttacg
     5401 aggcagtcca caggaatcag tgcgattcac ctttaaggtg aatcgtcatc aaaaaatcac
     5461 tcggtagcaa cgacccgaac cgaccaggat tgatttcccg gttctcagtt cgcaggcttt
     5521 tgagcgcgtc accttgacca ttgggtaact gccatcagcc gataagctaa acgggctgta
     5581 tagcggtaaa gcatcccaca cagtcgggct ggcatcaact ttgcaggaat agctcacgtc
     5641 actcatctca ctcgcgcctg ggttggatgg cagcgaaggc agattacgac gcagtttttt
     5701 actggcactt ttacccgcat taaaaacggg tacagtgcca ttgttgacgg tctgtacttc
     5761 ggtcatatac tcggtgtaca cttaatacac tctatactat tactgccgat tagtacattt
     5821 gtcaatcact ctttgcacaa ggtgtatgat atggactcag gagtacacca aacgtcatgc
     5881 caaccaataa agggagaata gcagtcactc tagaagctga aatttaccaa tggattgcta
     5941 accgagcgtc tgaggaagga agaccgttgg ctaatcttgc cgctttctta ctcacacgag
     6001 ttgttaaaga acaaatggaa caagaagcca aggacaacca agacaagcag ggggcagcat
     6061 gagcgaagac agactagcca gaatagaagc tgcgttagac agccaagttg cagtgaatgc
     6121 cgacctccgc acatcggtta cagaactccg cgcaaccgca gaagcattgt tgcaaacagt
     6181 tcaaatccat cagcagaact ttgaaattct taccgctagg caattacaaa ccgaagcacg
     6241 gcttgatgag taccaacgta ccactagcgc ggcactcgac agaattggcg cggtcttaga
     6301 ctacctcgtt aggcagcaaa acggttgagg tgagggatga gcgatgacta tctagacgga
     6361 tatcccgcaa gaggcccttt cgtcttcaag aattcccgtt tgactggcga tgctgctact
     6421 gaaactaaca actacatcga ctacgcaatt aacgccctca gctaattttg cttagtctag
     6481 gcccggatgg gtaagtggtt ttcagcttaa gtgttgggtt ctacttactt ctccgggtct
     6541 tgctctatct aaaaacattg gtttaacaag gagtattagg caaatgccag ttactgtcgc
     6601 tgcctctcgc ttgggaaccg ctgcgtttga ccaatcaccc gtcgaactgc gcgctaacta
     6661 ttctcgacct gcaggtcgac tctagaggat caattcgagc tcggtacccg gggatcctct
     6721 agatttaaga aggagatata catatgagta aaggagaaga acttttcact ggagttgtcc
     6781 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     6841 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     6901 tacctgttcc atggccaaca cttgtcacta ctttcgcgta tggtcttcaa tgctttgcga
     6961 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     7021 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     7081 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     7141 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     7201 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     7261 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     7321 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     7381 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     7441 tggatgaact atacaaataa atgtccagac ctgcaggcat gcaagctggg gatccttttt
     7501 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7561 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7621 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7681 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7741 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7801 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7861 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7921 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7981 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8041 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8101 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8161 aacctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8221 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8281 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8341 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8401 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8461 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     8521 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8581 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     8641 tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     8701 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcct
     8761 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     8821 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccc
     8881 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     8941 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     9001 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     9061 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     9121 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     9181 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     9241 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg
     9301 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     9361 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     9421 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     9481 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     9541 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     9601 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     9661 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     9721 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     9781 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     9841 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt
     9901 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     9961 ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
    10021 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
    10081 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg
    10141 ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga agctttctct
    10201 gagctgtaac agcctgaccg caacaaacga gaggatcgag accatccgct ccagattatc
    10261 cggctcctcc atgcgttgcc tctcggctcc tgctccggtt ttccatgcct tatggaactc
    10321 ctcgatccgc cagcgatggg tataaatgtc gatgacgcgc aaggcttggg ctagcgactc
    10381 gaccggttcg ctggtcagca acaaccattt caacggggtc tcacccttgg gcgggttaat
    10441 ctcctcggcc agcaccgcgt tgagcgtgat attcccctgt tttagcgtga tgcgcccact
    10501 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg
    10561 ctgtcggaat ggacgat
//
