LOCUS       V014940                 7330 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014940
VERSION     V014940
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..42
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    56..103
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             121..1983
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4055..4066
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     primer_bind     complement(4541..4557)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(4698..5153)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5180..5284
                     /label="AmpR promoter"
     CDS             5285..6142
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6316..6904
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7192..7213
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7228..7258
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7266..7282
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7290..7306
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa gatcagaagt
       61 tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg cgcagccgcc
      121 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
      181 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
      241 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
      301 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct tttctttgtg
      361 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
      421 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
      481 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
      541 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
      601 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
      661 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
      721 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
      781 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
      841 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
      901 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
      961 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
     1021 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
     1081 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
     1141 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
     1201 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
     1261 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
     1321 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
     1381 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
     1441 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
     1501 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
     1561 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
     1621 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
     1681 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg
     1741 aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc
     1801 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
     1861 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg
     1921 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa
     1981 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga
     2041 caacctctct gagggcattc gcgagtggtg ggacttgaaa cctggagccc cgaaacccaa
     2101 agccaaccag caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct
     2161 cggacccttc aacggactcg acaaggggga gcccgtcaac gcggcggatg cagcggccct
     2221 cgagcacgac aaggcctacg accagcagct caaagcgggt gacaatccgt acctgcggta
     2281 taaccacgcc gacgccgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa
     2341 cctcgggcga gcagtcttcc aggccaagaa gagggttctc gaacctcttg gtctggttga
     2401 ggaaggtgct aagacggctc ctggaaagaa acgtccggta gagcagtcgc cacaagagcc
     2461 agactcctcc tcgggcattg gcaagacagg ccagcagccc gctaaaaaga gactcaattt
     2521 tggtcagact ggcgactcag agtcagtccc cgacccacaa cctctcggag aacctccagc
     2581 aacccccgct gctgtgggac ctactacaat ggcttcaggc ggtggcgcac caatggcaga
     2641 caataacgaa ggcgccgacg gagtgggtaa tgcctcagga aattggcatt gcgattccac
     2701 atggctgggc gacagagtca tcaccaccag cacccgaaca tgggccttgc ccacctataa
     2761 caaccacctc tacaagcaaa tctccagtgc ttcaacgggg gccagcaacg acaaccacta
     2821 cttcggctac agcaccccct gggggtattt tgatttcaac agattccact gccatttctc
     2881 accacgtgac tggcagcgac tcatcaacaa caattgggga ttccggccca agagactcaa
     2941 cttcaagctc ttcaacatcc aagtcaagga ggtcacgacg aatgatggcg tcacgaccat
     3001 cgctaataac cttaccagca cggttcaagt cttctcggac tcggagtacc agttgccgta
     3061 cgtcctcggc tctgcgcacc agggctgcct ccctccgttc ccggcggacg tgttcatgat
     3121 tccgcagtac ggctacctaa cgctcaacaa tggcagccag gcagtgggac ggtcatcctt
     3181 ttactgcctg gaatatttcc catcgcagat gctgagaacg ggcaataact ttaccttcag
     3241 ctacaccttc gaggacgtgc ctttccacag cagctacgcg cacagccaga gcctggaccg
     3301 gctgatgaat cctctcatcg accagtacct gtattacctg aacagaactc agaatcagtc
     3361 cggaagtgcc caaaacaagg acttgctgtt tagccggggg tctccagctg gcatgtctgt
     3421 tcagcccaaa aactggctac ctggaccctg ttaccggcag cagcgcgttt ctaaaacaaa
     3481 aacagacaac aacaacagca actttacctg gactggtgct tcaaaatata accttaatgg
     3541 gcgtgaatct ataatcaacc ctggcactgc tatggcctca cacaaagacg acaaagacaa
     3601 gttctttccc atgagcggtg tcatgatttt tggaaaggag agcgccggag cttcaaacac
     3661 tgcattggac aatgtcatga tcacagacga agaggaaatc aaagccacta accccgtggc
     3721 caccgaaaga tttgggactg tggcagtcaa tctccagagc agcagcacag accctgcgac
     3781 cggagatgtg catgttatgg gagccttacc tggaatggtg tggcaagaca gagacgtata
     3841 cctgcagggt cctatttggg ccaaaattcc tcacacggat ggacactttc acccgtctcc
     3901 tctcatgggc ggctttggac ttaagcaccc gcctcctcag atcctcatca aaaacacgcc
     3961 tgttcctgcg aatcctccgg cagagttttc ggctacaaag tttgcttcat tcatcaccca
     4021 gtattccaca ggacaagtga gcgtggagat tgaatgggag ctgcagaaag aaaacagcaa
     4081 acgctggaat cccgaagtgc agtatacatc taactatgca aaatctgcca acgttgattt
     4141 cactgtggac aacaatggac tttatactga gcctcgcccc attggcaccc gttacctcac
     4201 ccgtcccctg taattgtgtg ttaatcaata aaccggttaa ttcgtgtcag ttgaactttg
     4261 gtctcatgtc gttattatct tatctggtca ccagatcccc gtagataagt agcatggcgg
     4321 gttaatcatt aactacagcc cgggcgttta aacagcgggc ggaggggtgg agtcgtgacg
     4381 tgaattacgt catagggtta gggaggtcct gtattagagg tcacgtgagt gttttgcgac
     4441 attttgcgac accatgtggt ctcgctgggg gggggggccc gagtgagcac gcagggtctc
     4501 cattttgaag cgggaggttt gaacgagcgc tggcgcgctc actggccgtc gttttacaac
     4561 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     4621 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4681 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4741 ttgttaaatc agctcatttt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     4801 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     4861 taagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4921 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4981 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5041 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5101 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5281 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5341 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5401 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5461 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5521 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5581 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5641 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5701 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5761 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5821 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5881 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5941 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6001 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6061 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6121 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6181 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6241 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6421 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6721 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6961 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7021 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7081 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7141 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7201 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7261 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7321 gcgcgccgat
//
