LOCUS V043521 7316 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043521 VERSION V043521 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 221..524 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 525..727 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 876..1254 /note="fusion between human cytomegalovirus intron A and human β-globin intron 2" /label="chimeric intron" CDS 2350..2370 /codon_start=1 /locus_tag="" /note="" /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /protein_id="" /transl_table=1 /translation="PKKKRKV" /label="SV40 NLS" CDS 2380..2433 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 2434..3870 /label="KLF4(NM_004235)" /note="KLF4(NM_004235)" /gene="KLF4" CDS 3886..3906 /codon_start=1 /locus_tag="" /note="" /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /protein_id="" /transl_table=1 /translation="PKKKRKV" /label="SV40 NLS" polyA_signal 4063..4539 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 4579..4719 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 4794..5249 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5531..5635 /gene="bla" /label="AmpR promoter" CDS 5636..6496 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 6667..7255 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cccacatttg cttctgacac aactgtgttc actagcaacc tcaaacagac 1381 accatgtaca acatgatgga gacggagctg aagccgccgg gcccgcagca aacttcgggg 1441 ggcggcggcg gcaactccac cgcggcggcg gccggcggca accagaaaaa cagcccggac 1501 cgcgtcaagc ggcccatgaa tgccttcatg gtgtggtccc gcgggcagcg gcgcaagatg 1561 gcccaggaga accccaagat gcacaactcg gagatcagca agcgcctggg cgccgagtgg 1621 aaacttttgt cggagacgga gaagcggccg ttcatcgacg aggctaagcg gctgcgagcg 1681 ctgcacatga aggagcaccc ggattataaa taccggcccc ggcggaaaac caagacgctc 1741 atgaagaagg ataagtacac gctgcccggc gggctgctgg cccccggcgg caatagcatg 1801 gcgagcgggg tcggggtggg cgccggcctg ggcgcgggcg tgaaccagcg catggacagt 1861 tacgcgcaca tgaacggctg gagcaacggc agctacagca tgatgcagga ccagctgggc 1921 tacccgcagc acccgggcct caatgcgcac ggcgcagcgc agatgcagcc catgcaccgc 1981 tacgacgtga gcgccctgca gtacaactcc atgaccagct cgcagaccta catgaacggc 2041 tcgcccacct acagcatgtc ctactcgcag cagggcaccc ctggcatggc tcttggctcc 2101 atgggttcgg tggtcaagtc cgaggccagc tccagccccc ctgtggttac ctcttcctcc 2161 cactccaggg cgccctgcca ggccggggac ctccgggaca tgatcagcat gtatctcccc 2221 ggcgccgagg tgccggaacc cgccgccccc agcagacttc acatgtccca gcactaccag 2281 agcggcccgg tgcccggcac ggccattaac ggcacactgc ccctctcaca catgggaggg 2341 ggaggttctc ccaaaaagaa acgaaaggtc gggtccggcg aggggcgggg ctccctgctc 2401 acctgtgggg acgtggaaga gaatcccggc cctatgaggc agccacctgg cgagtctgac 2461 atggctgtca gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga 2521 agggagaaga cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc 2581 cacatgaagc gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc 2641 gtggccacag acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg 2701 cccctacctc ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc 2761 tccaattcgc tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca 2821 gcctcctctt cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc 2881 ttcacctatc cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga 2941 ggcctcctct atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac 3001 atcaacgacg tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac 3061 ccggtgtaca ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc 3121 gtgctgaagg cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc 3181 gtcagcaaag gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg 3241 ccgccgcgca cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc 3301 gctggacccc ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg 3361 cagctcccca gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac 3421 tgtcaccctg ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca 3481 tccttcctgc ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg 3541 ccacccggtt cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc 3601 cggaaaagga ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag 3661 agttcccatc tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac 3721 tgggacggct gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa 3781 cacacggggc accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac 3841 cacctcgcct tacacatgaa gaggcatttt ggagggggag gttctcccaa aaagaaacga 3901 aaggtctaag gctggagcct cggtagccgt tcctcctgcc cgctgggcct cccaacgggc 3961 cctcctcccc tccttgcacc ggcccttcct ggtctttgcc ggggatcctc tagagtcgac 4021 ctgcagaagc ttgcctcgag cagcgctgct cgagagatct acgggtggca tccctgtgac 4081 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc 4141 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg 4201 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg 4261 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc 4321 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc 4381 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc 4441 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg 4501 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg 4561 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg 4621 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg 4681 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc 4741 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc 4801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 4861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 4921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 4981 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc 5041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 5101 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat 5161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 5221 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga 5281 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 5341 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 5401 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct 5461 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg 5521 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 5581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 5641 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 5701 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 5761 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 5821 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 5881 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 5941 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 6001 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 6061 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 6121 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 6181 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 6241 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 6301 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 6361 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 6421 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 6481 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 6541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 6601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 6661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 6721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 6781 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 6841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 6901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 6961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 7021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 7081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 7141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 7201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 7261 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt //