LOCUS       V043521                 7316 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043521
VERSION     V043521
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        221..524
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        525..727
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          876..1254
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /label="chimeric intron"
     CDS             2350..2370
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             2380..2433
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             2434..3870
                     /label="KLF4(NM_004235)"
                     /note="KLF4(NM_004235)"
                     /gene="KLF4"
     CDS             3886..3906
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    4063..4539
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   4579..4719
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4794..5249
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5531..5635
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5636..6496
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6667..7255
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt cccacatttg cttctgacac aactgtgttc actagcaacc tcaaacagac
     1381 accatgtaca acatgatgga gacggagctg aagccgccgg gcccgcagca aacttcgggg
     1441 ggcggcggcg gcaactccac cgcggcggcg gccggcggca accagaaaaa cagcccggac
     1501 cgcgtcaagc ggcccatgaa tgccttcatg gtgtggtccc gcgggcagcg gcgcaagatg
     1561 gcccaggaga accccaagat gcacaactcg gagatcagca agcgcctggg cgccgagtgg
     1621 aaacttttgt cggagacgga gaagcggccg ttcatcgacg aggctaagcg gctgcgagcg
     1681 ctgcacatga aggagcaccc ggattataaa taccggcccc ggcggaaaac caagacgctc
     1741 atgaagaagg ataagtacac gctgcccggc gggctgctgg cccccggcgg caatagcatg
     1801 gcgagcgggg tcggggtggg cgccggcctg ggcgcgggcg tgaaccagcg catggacagt
     1861 tacgcgcaca tgaacggctg gagcaacggc agctacagca tgatgcagga ccagctgggc
     1921 tacccgcagc acccgggcct caatgcgcac ggcgcagcgc agatgcagcc catgcaccgc
     1981 tacgacgtga gcgccctgca gtacaactcc atgaccagct cgcagaccta catgaacggc
     2041 tcgcccacct acagcatgtc ctactcgcag cagggcaccc ctggcatggc tcttggctcc
     2101 atgggttcgg tggtcaagtc cgaggccagc tccagccccc ctgtggttac ctcttcctcc
     2161 cactccaggg cgccctgcca ggccggggac ctccgggaca tgatcagcat gtatctcccc
     2221 ggcgccgagg tgccggaacc cgccgccccc agcagacttc acatgtccca gcactaccag
     2281 agcggcccgg tgcccggcac ggccattaac ggcacactgc ccctctcaca catgggaggg
     2341 ggaggttctc ccaaaaagaa acgaaaggtc gggtccggcg aggggcgggg ctccctgctc
     2401 acctgtgggg acgtggaaga gaatcccggc cctatgaggc agccacctgg cgagtctgac
     2461 atggctgtca gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga
     2521 agggagaaga cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc
     2581 cacatgaagc gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc
     2641 gtggccacag acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg
     2701 cccctacctc ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc
     2761 tccaattcgc tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca
     2821 gcctcctctt cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc
     2881 ttcacctatc cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga
     2941 ggcctcctct atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac
     3001 atcaacgacg tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac
     3061 ccggtgtaca ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc
     3121 gtgctgaagg cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc
     3181 gtcagcaaag gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg
     3241 ccgccgcgca cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc
     3301 gctggacccc ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg
     3361 cagctcccca gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac
     3421 tgtcaccctg ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca
     3481 tccttcctgc ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg
     3541 ccacccggtt cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc
     3601 cggaaaagga ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag
     3661 agttcccatc tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac
     3721 tgggacggct gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa
     3781 cacacggggc accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac
     3841 cacctcgcct tacacatgaa gaggcatttt ggagggggag gttctcccaa aaagaaacga
     3901 aaggtctaag gctggagcct cggtagccgt tcctcctgcc cgctgggcct cccaacgggc
     3961 cctcctcccc tccttgcacc ggcccttcct ggtctttgcc ggggatcctc tagagtcgac
     4021 ctgcagaagc ttgcctcgag cagcgctgct cgagagatct acgggtggca tccctgtgac
     4081 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     4141 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     4201 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     4261 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     4321 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     4381 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     4441 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     4501 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     4561 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4621 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4681 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4741 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     4801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4981 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     5041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5101 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     5161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5221 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     5281 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5341 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5401 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5461 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5521 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5641 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5701 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5761 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5821 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5881 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5941 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6001 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6061 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6121 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6181 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6241 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6301 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6361 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6421 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6481 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6781 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7261 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
