LOCUS       V017343                 4197 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017343
VERSION     V017343
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             152..424
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"
     protein_bind    443..459
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             661..690
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    complement(867..886)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     CDS             954..1703
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             1743..2321
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     terminator      2370..2456
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2621..3209)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3297..3391)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3415..4071)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4072..4174)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcagtc ctttgattct aataaattgg atttttgtca cactattgta tcgctgggaa
       61 tacaattact taacataagc acctgtagga tcgtacaggt ttacgcaaga aaatggtttg
      121 ttatagtcga ataaacgcaa gggaggttgg tatggtacgc tggactttgt gggataccct
      181 cgctttcctg ctcctgttga gtttattgct gccgtcattg cttattatgt tcatcccgtc
      241 aacattcaaa cggcctgtct catcatggaa ggcgctgaat ttacggaaaa cattattaat
      301 ggcgtcgagc gtccggttaa agccgctgaa ttgttcgcgt ttaccttgcg tgtacgcgca
      361 ggaaacactg acgttcttac tgacgcagaa gaaaacgtgc gtcaaaaatt acgtgcggaa
      421 ggagtgagac gtctgtgtgg aattgtgagc ggataacaat ttcacacagg gccctcggac
      481 accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag gaggaacgag
      541 ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa caatgaaaag
      601 gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt ccaagcagag
      661 gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca gttgaaacac
      721 aaacttgaac agctacggaa ctcttgtgcg taaggaaaag taaggaaaac gattccttct
      781 aacagaaatg tcctgagcaa tcacctatga actgtcgact cgagcatagc atttttatcc
      841 ataagattag cggatctaac ctttacaatt gtgagcgctc acaattatga tagattcaat
      901 tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaaggta cccatgaaaa
      961 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata
     1021 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac
     1081 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaattcta gataattacc
     1141 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag
     1201 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa
     1261 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta
     1321 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa
     1381 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc
     1441 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt
     1501 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata
     1561 tttcaaaaat attaggctgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa
     1621 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa
     1681 ttgattgccc atactttaaa aattaagtac gggatccccg aataaacgca agggaggttg
     1741 gtatgactat aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag
     1801 gtattctaag tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag
     1861 aaaataacct tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg
     1921 atgatactga aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc
     1981 tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag
     2041 tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg
     2101 aaattacaat gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag
     2161 aatatgtaac agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt
     2221 gtcatcgtat tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt
     2281 ctatgcctat taatgaacag tttaaaaaag cagtcttaaa ttaatgttgt taagcttcgc
     2341 ggccgcttaa ttaattaatc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
     2401 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     2461 cgccctagac ctagggcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     2821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3061 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3241 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgactagt gcttggattc
     3301 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     3361 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     3421 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     3481 atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     3541 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     3601 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     3661 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     3721 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     3781 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     3841 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     3901 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     3961 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     4021 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     4081 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     4141 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatc
//
