LOCUS       62056_23370       11521 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7606..8322
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    8397..8959
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8962..9558
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        9759..9863
                     /label=AmpR promoter
     CDS             9864..10721
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10895..11483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
     7561 gtctagcata tgggcgcgcc ctcagcatcg attcaattcg ccaccatggt gagcaagggc
     7621 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     7681 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     7741 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     7801 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     7861 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     7921 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     7981 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     8041 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     8101 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     8161 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     8221 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     8281 accgccgccg ggatcactct cggcatggac gagctgtaca agtagtctag agtcgacccg
     8341 ggcggccgca actaacttaa gctagcaacg gtttccctct agcgggatca attccgcccc
     8401 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     8461 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     8521 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     8581 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     8641 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     8701 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     8761 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     8821 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     8881 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     8941 tttgaaaaac acgataatac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     9001 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     9061 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     9121 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     9181 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     9241 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     9301 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     9361 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     9421 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     9481 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     9541 acccgcaagc ccggtgcctg agaattggca agctgcttac atagaactcg cggcgattgg
     9601 catgccgcct taaaattttt attttatttt tcttttcttt tccgaatcgg attttgtttt
     9661 taatatttca aaaaaaaaaa aaaaaaaaaa aaaaacgcgt cgaggggaat taattcttga
     9721 agacgaaagg gccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     9781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9901 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9961 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10021 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10081 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
    10141 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    10201 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    10261 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    10321 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    10381 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10441 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10501 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10561 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10621 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10681 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    10741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10801 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10861 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10921 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10981 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11041 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11101 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    11161 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    11221 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11281 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11341 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11401 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11461 caggggggcg gagcctatgg aaaaacgcca gcaacgcgag ctctaatacg actcactata
    11521 g
//
