LOCUS       Super_PiggyBac_T        8531 bp DNA     circular SYN 19-MAY-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Super PiggyBac Transposase.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   caoheibi
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..1993
                     /label=chicken 5'-HS4 beta-globin insulator (HS4)
                     /note="Insulators are DNA sequence elements that prevent 
                     inappropriate interactions between adjacent chromatin 
                     domains. One type of insulator establishes domains that 
                     separate enhancers and promoters to block their 
                     interaction, whereas a second type creates a barrier 
                     against the spread of heterochromatin."
     primer_bind     2449..2465
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2492..3197
                     /label=Polr2A promoter
                     /note="RNA polymerase II large subunit promoter"
     intron          3442..3538
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             3614..5395
                     /label=hyPBase
                     /note="Hyperactive version of piggyBac transposase (PBase) 
                     created by mutagenesis"
     polyA_signal    complement(5424..5558)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(5675..5693)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5714..5730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5738..5754)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5762..5792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5807..5828)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6116..6704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6878..7735)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7736..7840)
                     /label=AmpR promoter
     rep_origin      7867..8322
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8463..8479
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8489..8507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
       61 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
      121 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
      181 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
      241 gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga acggcctggg
      301 ttgcaaagga gcacagtgct catccagatc caaccccctg ctatgtgcag ggtcatcaac
      361 cagcagccca ggctgcccag agccacatcc agcctggcct tgaatgcctg cagggatggg
      421 gcatccacag cctccttggg caacctgttc agtgcgtcac caccctctgg gggaaaaact
      481 gcctcctcat atccaaccca aacctcccct gtctcagtgt aaagccattc ccccttgtcc
      541 tatcaagggg gagtttgctg tgacattgtt ggtctggggt gacacatgtt tgccaattca
      601 gtgcatcacg gagaggcaga tcttggggat aaggaagtgc aggacagcat ggacgtggga
      661 catgcaggtg ttgagggctc tgggacactc tccaagtcac agcgttcaga acagccttaa
      721 ggataagaag ataggataga aggacaaaga gcaagttaaa acccagcatg gagaggagca
      781 caaaaaggcc acagacactg ctggtccctg tgtctgagcc tgcatgtttg atggtgtctg
      841 gatgcaagca gaaggggtgg aagagcttgc ctggagagat acagctgggt cagtaggact
      901 gggacaggca gctggagaat tgccatgtag atgttcatac aatcgtcaaa tcatgaaggc
      961 tggaaaagcc ctccaagatc cccaagacca accccaaccc acccaccgtg cccactggcc
     1021 atgtccctca gtgccacatc cccacagttc ttcatcacct ccagggacgg tgaccccccc
     1081 acctccgtgg gcagctgtgc cactgcagca ccgctctttg gagaaggtaa atcttgctaa
     1141 atccagcccg accctcccct ggcacaacgt aaggccatta tctctcatcc aactccagga
     1201 cggagtcagt gagaatattg cctccctggc gagctcacgg ggacagcccc cccccaaagc
     1261 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
     1321 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
     1381 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
     1441 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
     1501 cagaatcata gaacggcctg ggttgcaaag gagcacagtg ctcatccaga tccaaccccc
     1561 tgctatgtgc agggtcatca accagcagcc caggctgccc agagccacat ccagcctggc
     1621 cttgaatgcc tgcagggatg gggcatccac agcctccttg ggcaacctgt tcagtgcgtc
     1681 accaccctct gggggaaaaa ctgcctcctc atatccaacc caaacctccc ctgtctcagt
     1741 gtaaagccat tcccccttgt cctatcaagg gggagtttgc tgtgacattg ttggtctggg
     1801 gtgacacatg tttgccaatt cagtgcatca cggagaggca gatcttgggg ataaggaagt
     1861 gcaggacagc atggacgtgg gacatgcagg tgttgagggc tctgggacac tctccaagtc
     1921 acagcgttca gaacagcctt aaggataaga agataggata gaaggacaaa gagcaagtta
     1981 aaacccagca tggagaggag cacaaaaagg ccacagacac tgctggtccc tgtgtctgag
     2041 cctgcatgtt tgatggtgtc tggatgcaag cagaaggggt ggaagagctt gcctggagag
     2101 atacagctgg gtcagtagga ctgggacagg cagctggaga attgccatgt agatgttcat
     2161 acaatcgtca aatcatgaag gctggaaaag ccctccaaga tccccaagac caaccccaac
     2221 ccacccaccg tgcccactgg ccatgtccct cagtgccaca tccccacagt tcttcatcac
     2281 ctccagggac ggtgaccccc ccacctccgt gggcagctgt gccactgcag caccgctctt
     2341 tggagaaggt aaatcttgct aaatccagcc cgaccctccc ctggcacaac gtaaggccat
     2401 tatctctcat ccaactccag gacggagtca gtgagaatat tcggccgctc tagaactagt
     2461 ggatcccccg ggctgcagga attcgatggc cgcttgggcg ggatatctag cgccaaggtt
     2521 ccgtaaagct ctctattaga ggactggcgg tgccccatca ccctgggagg cctctccaca
     2581 aatatcggct acttccaatt gattggacgc gccatcttgt ctgcttatgc atattcagag
     2641 gatcctgaat attcatgagc gagggccgtg cggcccctcc ctccaaccct cccccggaac
     2701 aagctccgga gaacccgaca ggccccgcct tctttaccga tgcgtagaac aaaccatttt
     2761 ccgggttggg gggggaaatt aatgagagac tttagctgaa aatgagcatg gacgccaaag
     2821 ctgagtaaag atggcttaac tttatcctcc attctgtaat ccgtcagctt gagtgtacgg
     2881 gaagtcggcg aggggggcgg caggctcaga aacattctcc tcctcctgtc gcgtcagaaa
     2941 gaacacccaa ccagggagcc ggagccctgg cgtcaacatc tctggcgcgc gcgctccatg
     3001 taggccggtg cgggcggccc cgtagcgcaa gggagggcgg gaaaggaagg ggcgggacac
     3061 aagggcgaat ctataaaggg cgtcattcag ccagttctct cctcagaagc gccgagagcg
     3121 cgaccgggac ggttggagaa gaaggtggct cccggaaggg ggagagacaa actgccgtaa
     3181 cctctgccgt tcaggatccc ggccatcaag cttatcgatg ctgccgcagc aaaagcagga
     3241 gcagatgccg ccgtcgcagg cgaagatgtc gcagacggag gaggcgatgc tgccggcgga
     3301 ggaggcgaag taagtagagg gctgggctgg gctgtggggg gtgtggggtg cgggactggg
     3361 cagtctggga gtccctctca ccacttttct tacctttcta ggatgctgcc tcgaggaact
     3421 gaaaaaccag aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga
     3481 tccggtggtg gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc
     3541 ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat tgtacccaat tcgttaaggc
     3601 caaattggcc accatgggct ctagcctgga cgacgagcac atcctgagcg ccctgctgca
     3661 gagcgacgac gaactggtgg gcgaggacag cgacagcgag gtcagcgacc acgtgtccga
     3721 ggacgacgtg cagtccgaca ccgaggaagc cttcatcgac gaggtgcacg aagtgcagcc
     3781 taccagcagc ggctccgaga tcctggacga gcagaacgtg atcgagcagc ctggcagctc
     3841 cctggccagc aacagaatcc tgaccctgcc ccagagaacc atcagaggca agaacaagca
     3901 ctgctggtcc acctccaaga gcaccaggcg gagcagagtg tccgccctga acatcgtgcg
     3961 gagccagagg ggccccacca gaatgtgcag aaacatctac gaccccctgc tgtgcttcaa
     4021 gctgttcttc accgacgaga tcatcagcga gatcgtgaag tggaccaacg ccgagatcag
     4081 cctgaagagg cgggagagca tgaccagcgc caccttcaga gacaccaacg aggacgagat
     4141 ctacgccttc ttcggcatcc tggtgatgac cgccgtgaga aaggacaacc acatgagcac
     4201 cgacgacctg ttcgacagat ccctgagcat ggtgtacgtg tccgtgatga gcagagacag
     4261 attcgacttc ctgatcagat gcctgagaat ggacgacaag agcatcagac ccaccctgcg
     4321 ggagaacgac gtgttcaccc ccgtgcggaa gatctgggac ctgttcatcc accagtgcat
     4381 ccagaactac acccctggcg cccacctgac catcgatgag cagctgctgg gcttcagagg
     4441 cagatgcccc ttcagagtgt acatccccaa caagcccagc aagtacggca tcaagatcct
     4501 gatgatgtgc gacagcggca ccaagtacat gatcaacggc atgccctacc tgggcagagg
     4561 cacccagaca aacggcgtgc ccctgggcga gtactacgtg aaagaactga gcaagcctgt
     4621 gcatggcagc tgcaggaaca tcacctgcga caactggttc accagcatcc ccctggccaa
     4681 gaacctgctg caggaaccct acaagctgac catcgtgggc accgtgcgga gcaacaagcg
     4741 ggagatccca gaggtgctga agaacagcag atccagacct gtgggaacaa gcatgttctg
     4801 cttcgacggc cccctgaccc tggtgtccta caagcccaag cccgccaaga tggtgtacct
     4861 gctgtccagc tgcgacgagg acgccagcat caacgagagc accggcaagc cccagatggt
     4921 gatgtactac aaccagacca agggcggcgt ggacaccctg gaccagatgt gcagcgtgat
     4981 gacctgcagc agaaagacca acagatggcc catggccctg ctgtacggca tgatcaatat
     5041 cgcctgcatc aacagcttca tcatctacag ccacaacgtg tccagcaagg gcgagaaggt
     5101 gcagagccgg aagaaattca tgcggaacct gtacatgagc ctgacctcca gcttcatgag
     5161 aaagagactg gaagccccca ccctgaagag atacctgcgg gacaacatca gcaacatcct
     5221 gcccaaggaa gtgccaggaa caagcgacga cagcaccgag gaacccgtga tgaagaagag
     5281 gacctactgc acctactgtc ccagcaagat cagaagaaag gccaacgcca gctgcaagaa
     5341 atgcaaaaaa gtgatctgcc gggagcacaa catcgacatg tgccagagct gtttctgagg
     5401 ccgtaacggc cgccagaatt ggggatccag acatgataag atacattgat gagtttggac
     5461 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5521 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5581 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgacct
     5641 gcaggcatgc aagctcggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     5701 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5761 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5821 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5881 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6541 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6901 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7201 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7261 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7321 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7861 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7921 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7981 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8041 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8101 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8161 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8221 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8281 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
     8341 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8401 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8461 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
     8521 ccaccgcggt g
//
