LOCUS       40924_48723        8335 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector RSFmob, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Katashkina JI, Kuvaeva TM, Skorokhodova AY, Biryukova IV, Golubeva 
            LI, Mashko SV.
  TITLE     Construction of Stably Maintained mob- Derivatives of RSF1010 
            Plasmid Lacking All the Known DNA Sequences Involved in the 
            Mobilization Process
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Katashkina JI, Kuvaeva TM, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2007) Ajinomoto-Genetika Research Institute, 1st 
            Dorozhny Pr., Moscow 117545, Russia
REFERENCE   3  (bases 1 to 8335)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8335)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2007) Ajinomoto-Genetika Research Institute, 1st Dorozhny 
            Pr., Moscow 117545, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..866
                     /codon_start=1
                     /gene="strA"
                     /product="streptomycin resistance protein A"
                     /label=strA
                     /protein_id="ABP62943.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            63..866
                     /gene="strA"
                     /label=strA
     CDS             866..1702
                     /codon_start=1
                     /gene="strB"
                     /product="streptomycin resistance protein B"
                     /label=strB
                     /protein_id="ABP62944.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            866..1702
                     /gene="strB"
                     /label=strB
     rep_origin      complement(2346..2740)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     protein_bind    2810..2831
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2846..2876
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    2884..2900
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2925..2947
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2955..4034
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             4059..5027
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5091..5303
                     /codon_start=1
                     /gene="orfE"
                     /product="unknown protein E"
                     /label=orfE
                     /note="hypothetical protein"
                     /protein_id="ABP62947.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     gene            5091..5303
                     /gene="orfE"
                     /label=orfE
     CDS             5305..5511
                     /codon_start=1
                     /gene="orfF"
                     /product="repressor protein F"
                     /label=orfF
                     /note="transcriptional regulator of repAC operon"
                     /protein_id="ABP62948.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            5305..5511
                     /gene="orfF"
                     /label=orfF
     CDS             5541..6377
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6367..7215
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             7526..8314
                     /codon_start=1
                     /gene="sul"
                     /product="sulfonamide resistance protein"
                     /label=sul
                     /protein_id="ABP62951.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVI
                     DLVRHPAIPTPRLFRPTQKSRVCAGAGRAQADGIPVSLDSYQPATQAYALSRGVAYLND
                     IRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGA
                     GIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRG
                     PGVSGPRHSLQSLPPPQVELTSSAHTSRAPCATGWRYWRR"
     gene            7526..8314
                     /gene="sul"
                     /label=sul
ORIGIN
        1 aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
       61 cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
      121 gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
      181 cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
      241 gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
      301 tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
      361 aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
      421 gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
      481 atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
      541 tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
      601 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
      661 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
      721 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
      781 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
      841 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
      901 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
      961 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     1021 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     1081 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     1141 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     1201 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     1261 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     1321 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     1381 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     1441 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     1501 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     1561 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     1621 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     1681 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     1741 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     1801 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     1861 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     1921 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     1981 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     2041 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     2101 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     2161 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     2221 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     2281 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     2341 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     2401 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     2461 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     2521 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     2581 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     2641 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     2701 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     2761 gatcattcag cccggctcat agatctgcgg gcagtgagcg caacgcaatt aatgtaagtt
     2821 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     2881 gaattgtgag cggataacaa tttcacacag gatctagaaa taattttgtt taactttaag
     2941 aaggagatat acatatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     3001 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     3061 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     3121 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     3181 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     3241 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     3301 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     3361 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     3421 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     3481 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     3541 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     3601 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     3661 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     3721 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     3781 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     3841 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     3901 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     3961 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4021 tggaaagcgg gcagtgagcg gatccggggg gtggcccgat gaagaacgac aggactttgc
     4081 aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg
     4141 acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca
     4201 cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc
     4261 aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga
     4321 aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat
     4381 gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg
     4441 ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg
     4501 gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc
     4561 tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc
     4621 agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac
     4681 tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga
     4741 tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc
     4801 agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca
     4861 gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca
     4921 gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg
     4981 caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt
     5041 ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg
     5101 aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg
     5161 gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg
     5221 ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc
     5281 ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg
     5341 ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg
     5401 atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg
     5461 gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact
     5521 gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg
     5581 cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg
     5641 cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga
     5701 ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc
     5761 tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc
     5821 tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca
     5881 gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc
     5941 gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg
     6001 gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta
     6061 tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc
     6121 aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga
     6181 gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg
     6241 tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc
     6301 ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg
     6361 tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg
     6421 cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag
     6481 ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg
     6541 ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc
     6601 ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa
     6661 cccaagtggg aggccgtcac cgctgaatgc catgtggtca aaggtagcta tcgggcgctg
     6721 gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc
     6781 atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt
     6841 cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac
     6901 cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag
     6961 gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc
     7021 gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca
     7081 gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag
     7141 ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg
     7201 cccaaggcgg caggctgacc ccccccactc tattgtaaac aagacatttt tatcttttat
     7261 attcaatggc ttattttcct gctaattggt aataccatga aaaataccat gctcagaaaa
     7321 ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc cataggccgc
     7381 tttcctggct ttgcttccag atgtatgctc ttctgctcct gcagctaatg gatcaccgca
     7441 aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgat actcatgctc
     7501 gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa
     7561 cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg cagccattgc
     7621 gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctggtcc ggcatccagc
     7681 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatgcg ccggtgctgg
     7741 acgcgctcag gcagatggca ttcccgtctc gctcgacagt tatcaacccg cgacgcaagc
     7801 ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc cagacgctgc
     7861 gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt cggtgcaaga
     7921 cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca ttgcggcgtt
     7981 ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc gccttgtcct
     8041 tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct cggtgctggc
     8101 gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg tttcgcgcaa
     8161 atcctttctg cgcgcgctca caggccgtgg tccgggggtg tcggggccgc gacactcgct
     8221 gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga gccgcgcccc
     8281 ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat tcgtt
//
