LOCUS       40924_48673        5087 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Control vector RD29A-LUC-NOS-At5g52310, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Kovtun Y, Chiu WL, Tena G, Sheen J.
  TITLE     Functional analysis of oxidative stress-activated mitogen-activated 
            protein kinase cascade in plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
  PUBMED    10717008
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Hwang I, Sheen J.
  TITLE     Two-component circuitry in Arabidopsis cytokinin signal transduction
  JOURNAL   Nature 413 (6854), 383-389 (2001)
  PUBMED    11574878
REFERENCE   3  (bases 1 to 5087)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   4  (bases 1 to 5087)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5087)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages: 
            "2940-2945"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nature"; 
            date: "2001"; volume: "413"; issue: "6854"; pages: "383-389"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..3023
                     /label=RD29A enhancer/promoter
                     /note="RD29A enhancer/promoter"
                     /regulatory_class="enhancer"
     CDS             3024..4673
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4831..5082
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatcccccg accgactact aataatagta agttacattt
     2701 taggatggaa taaatatcat accgacatca gtttgaaaga aaagggaaaa aaagaaaaaa
     2761 taaataaaag atatactacc gacatgagtt ccaaaaagca aaaaaaaaga tcaagccgac
     2821 acagacacgc gtagagagca aaatgacttt gacgtcacac cacgaaaaca gacgcttcat
     2881 acgtgtccct ttatctctct cagtctctct ataaacttag tgagaccctc ctctgtttta
     2941 ctcacaaata tgcaaactag aaaacaatca tcaggaataa agggtttgat tacttctatt
     3001 ggaaagaaaa aaatctttgg accatggaag acgccaaaaa cataaagaaa ggcccggcgc
     3061 cattctatcc tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat
     3121 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt
     3181 acgcggaata cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
     3241 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
     3301 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
     3361 aattgctcaa cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt
     3421 tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg
     3481 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
     3541 ctcccggttt taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg
     3601 cactgataat gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata
     3661 gaactgcctg cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
     3721 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
     3781 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
     3841 ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt
     3901 cattcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
     3961 cttctggggg cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc
     4021 ttccagggat acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac
     4081 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
     4141 ttgtggatct ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca
     4201 gaggacctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
     4261 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
     4321 tcatagttga ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg
     4381 aattggaatc gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc
     4441 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
     4501 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
     4561 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
     4621 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaaaatg
     4681 taactgtatt cagcgatgac gaaattctta gctattgtaa tattatatgc aaattgatga
     4741 atggtaattt tgtaattgtg ggtcactgta ctattttaac gaataataaa atcaggtata
     4801 ggtaactaaa aaggaattcg agctcgaatt gatcgttcaa acatttggca ataaagtttc
     4861 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgattacg
     4921 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4981 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     5041 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tctctag
//
