LOCUS       .                       8001 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1440..2618
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             2777..3484
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        3535..3746
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             3759..4027
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4060..4455
                     /label="BSD"
                     /note="blasticidin S deaminase"
     polyA_signal    4572..4704
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5980..5996)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6004..6020)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6028..6058)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6073..6094)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6382..6970)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7144..8001)
                     /label="AmpR"
                     /note="β-lactamase"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agtattatgc ccagtacatg
     1381 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1441 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     1501 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     1561 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     1621 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     1681 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     1741 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     1801 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     1861 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     1921 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     1981 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     2041 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     2101 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     2161 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     2221 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     2281 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     2341 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     2401 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     2461 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     2521 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     2581 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg gtacgctagc
     2641 gtttaaactt aagcttggta ccgaattcgc caccatgtcc gtcctgacgc cgctgctgct
     2701 gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga tccattcgtt
     2761 gggggatcca ccggtcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     2821 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     2881 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     2941 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3001 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     3061 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     3121 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     3181 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     3241 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     3301 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     3361 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     3421 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     3481 caagtaggga tccgcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag
     3541 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
     3601 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3661 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3721 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
     3781 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     3841 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     3901 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     3961 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     4021 acagatccaa gctgtgaccg gcgcctacgc taggccacca tggccaagcc tttgtctcaa
     4081 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     4141 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     4201 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     4261 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     4321 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     4381 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     4441 tatgtgtggg agggctaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4501 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4561 ttgtatcagt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4681 tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc
     4741 tatctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc
     4801 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc
     4861 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga
     4921 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg
     4981 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     5041 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     5101 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     5161 tttggggggg ggctgtccct gatatctata acaagaaaat atatatataa taagttatca
     5221 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     5281 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     5341 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     5401 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     5461 tcaattttac gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg
     5521 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
     5581 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact
     5641 gctgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
     5701 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc
     5761 ggcttttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
     5821 ccggcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
     5881 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac
     5941 cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6001 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6061 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6181 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6781 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7981 acggaaatgt tgaatactca t
//
