LOCUS V040566 9357 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040566 VERSION V040566 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" promoter 2221..2432 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" intron 2433..3371 /note="intron upstream of the start codon of human EF-1α" /label="EF-1α intron A" regulatory 3387..3396 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 3396..3425 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 3432..4034 /label="Arfrp1(NM_001165991)" /note="Arfrp1(NM_001165991)" /gene="Arfrp1" misc_feature 4151..4700 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 4703..5302 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5326..5914 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6121..6754 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6882..6898) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6906..6922 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6930..6960) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6975..6996 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7284..7872) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8043..8903) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8904..9008) /gene="bla" /label="AmpR promoter" polyA_signal 9056..9190 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt 2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3361 ttccatttca ggtgtcgtga ggatccgcca ccatggaaca aaaactcatc tcagaagagg 3421 atctgacgcg tatgtacacg ctgctttcgg gattgtacaa gtacatgttc cagaaggatg 3481 aatactgcat cctgatcctg ggcctggaca atgctgggaa gacgactttc ctggaacagt 3541 caaaaacacg ctttaacaag aactacaagg ggatgagtct atccaaaatc accactaccg 3601 tgggtctaaa cattggcact gtggacgtgg gaaaggctcg tctcatgttt tgggacttag 3661 gtgggcagga agagctgcag tctttgtggg acaagtacta tgcagagtgc catggtgtca 3721 tctatgtaat tgattccact gatgaagaaa ggctgtcaga atcaaaagag gcatttgaga 3781 aggtggtttc gagtgaagca ctggacggtg ttcccatcct ggtgttggcc aacaagcagg 3841 atgtggagac ttgcctctcc attcctgaca tcaagactgc attcagtgac tgtacctgta 3901 agattggccg gcgagattgt ctgacccagg cctgctctgc cctcacaggc aaaggagttc 3961 gagagggcat cgaatggatg gtgaagtgtg tcgtgcggaa tgttcaccgg ccaccacggc 4021 agagggacat cacataagaa ttcctcgagg gcggccgctc tagagtcgac gggccgcggt 4081 aacaattgtt aactaactta agctagcaac ggtttccctc tagcgggatc aattccgccc 4141 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 4201 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 4261 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 4321 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 4381 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 4441 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 4501 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 4561 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 4621 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc 4681 ctttgaaaaa cacgataata ccatgaccga gtacaagccc acggtgcgcc tcgccacccg 4741 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac 4801 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt 4861 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt 4921 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc 4981 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct 5041 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga 5101 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg 5161 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg 5221 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat 5281 gacccgcaag cccggtgcct gacccgggcg gggcgcgtct ggaacaatca acctctggat 5341 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 5401 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5461 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5521 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5581 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 5641 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 5701 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 5761 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 5821 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 5881 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc 5941 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct 6001 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa 6061 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac 6121 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca 6181 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 6241 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 6301 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 6361 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 6421 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 6481 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 6541 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 6601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6721 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6781 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag 6841 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 6901 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 6961 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 7021 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7081 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 7141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 7201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 7261 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 7321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 7381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 7441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 7501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 7561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 7621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 7681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 7741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 7801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 7861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 7921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 7981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 8041 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 8101 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 8161 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 8221 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 8281 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 8341 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 8401 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 8461 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 8521 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 8581 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 8641 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 8701 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 8761 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 8821 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 8881 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 8941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 9001 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt 9061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 9121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 9181 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata 9241 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga 9301 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt //