LOCUS       40924_2284        4413 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector OpiE2-iFlp-Ieterm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Vidigal J, Teixeira AP.
  TITLE     RMCE-based insect cell platform to produce membrane proteins 
            captured on HIV-1 Gag virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   van den Heuvel JJ, Bleckmann M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2017) Structure and Function of Proteins, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Lower Saxony 38124, Germany
REFERENCE   3  (bases 1 to 4413)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2017) Structure and Function of Proteins, Helmholtz Centre 
            for Infection Research, Inhoffenstrasse 7, Braunschweig, Lower 
            Saxony 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: VectorNTI v. 8
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..569
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     CDS             582..1877
                     /codon_start=1
                     /label=FLPo
                     /note="nuclear-targeted site-specific recombinase"
                     /translation="MAPKKKRKVMPQFGILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYYGQKHQSDITDIVSRLQLQFESSEEADKGNSRSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     terminator      1893..2199
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     rep_origin      complement(2553..3141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3608..3986)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4171..4275)
                     /label=AmpR promoter
ORIGIN
        1 atcatcgata agctagcgga tcatgatgat aaacaatgta tggtgctaat gttgcttcaa
       61 caacaattct gttgaactgt gttttcatgt ttgccaacaa gcacctttat actcggtggc
      121 ctccccacca ccaacttttt tgcactgcaa aaaaacacgc ttttgcacgc gggcccatac
      181 atagtacaaa ctctacgttt cgtagactat tttacataaa tagtctacac cgttgtatac
      241 gctccaaata cactaccaca cattgaacct ttttgcagtg caaaaaagta cgtgtcggca
      301 gtcacgtagg ccggccttat cgggtcgcgt cctgtcacgt acgaatcaca ttatcggacc
      361 ggacgagtgt tgtcttatcg tgacaggacg ccagcttcct gtgttgctaa ccgcagccgg
      421 acgcaactcc ttatcggaac aggacgcgcc tccatatcag ccgcgcgtta tctcatgcgc
      481 gtgaccggac acgaggcgcc cgtcccgctt atcgcgccta taaatacagc ccgcaacgat
      541 ctggtaaaca cagttgaaca gcatctgttc gaagagctcc catggctccc aagaaaaagc
      601 gcaaggtcat gccccagttc ggtatcctgt gcaagacccc ccccaaggtg ctcgtgcgtc
      661 agttcgtcga gcgtttcgag cgcccctccg gcgagaagat cgcttcctgc gctgctgagc
      721 tgacctacct gtgctggatg atcacccaca acggcaccgc tatcaagcgt gctaccttca
      781 tgtcctacaa caccatcatc tccaactccc tgtccttcga catcgtgaac aagtccctgc
      841 agttcaagta caagacccag aaggctacca tcctcgaggc ttccctgaag aagctgatcc
      901 ccgcttggga gttcaccatc atcccttact acggccagaa gcaccagtcc gacatcaccg
      961 acatcgtgtc ccgtctgcaa ctccagttcg agtcctccga ggaagctgac aagggcaact
     1021 cccgttccaa gaagatgctg aaggctctgc tgtccgaggg cgagtccatc tgggagatca
     1081 ccgagaagat cctgaactcc ttcgagtaca cctcccgttt caccaagacc aagaccctgt
     1141 accagttcct gttcctggcc accttcatca actgcggtcg tttctccgac atcaagaacg
     1201 tggaccccaa gtccttcaag ctggtgcaga acaagtacct gggtgtcatc atccagtgcc
     1261 tggtcaccga gactaagacc tccgtgtccc gccacatcta cttcttctcc gctcgtggtc
     1321 gtatcgaccc cctggtgtac ctggacgagt tcctgcgcaa ctccgagccc gtgctgaagc
     1381 gtgtgaaccg taccggcaac tcctccagca acaagcaaga gtaccagctg ctgaaggaca
     1441 acctcgtgcg ctcctacaac aaggctctga agaagaacgc tccctactcc atcttcgcta
     1501 tcaagaacgg tcccaagtcc cacatcggtc gtcacctgat gacatcgttc ttgtccatga
     1561 agggcctgac cgagctgacc aacgtcgtgg gcaactggtc cgacaagcgc gcttccgctg
     1621 tggctcgtac cacctacacc caccagatca ccgctatccc cgaccactac ttcgctctgg
     1681 tgtcccgtta ctacgcttac gaccccatct ccaaggaaat gatcgctctg aaggacgaga
     1741 ctaaccccat cgaggaatgg cagcacatcg agcagctgaa gggttccgct gagggttcca
     1801 tccgttaccc cgcttggaac ggtatcatct cccaagaggt gctggactac ctgtcctcct
     1861 acatcaaccg tcgtatcgtc gactagaagc ttttatacat atattttgaa tttaattaat
     1921 tatacatata ttttatatta tttttgtctt ttattatcga ggggccgttg ttggtgtggg
     1981 gttttgcata gaaataacaa tgggagttgg cgacgttgct gcgccaacac cacctccctt
     2041 ccctcctttc atcatgtatc tgtagataaa ataaaatatt aaacctaaaa acaagaccgc
     2101 gcctatcaac aaaatgatag gcattaactt gccgctgacg ctgtcactaa cgttggacga
     2161 tttgccgact aaaccttcat cgcccagtaa ccaatctagc aattgcattc attttatgtt
     2221 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     2281 tatggctgat tatgatcctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2341 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2401 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     2461 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     2521 tgcaccatat gtcgggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3241 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3301 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3361 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3421 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3481 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3541 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3601 agtgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3661 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3721 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3781 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3841 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3901 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     3961 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4021 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4081 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4141 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4201 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4261 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4321 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     4381 ggtcgatcga ccaattctca tgtttgacag ctt
//
