LOCUS       40924_1869        7272 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector LUCTRAP-3(GW), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Calderon-Villalobos LI, Kuhnle C, Li H, Rosso M, Weisshaar B, 
            Schwechheimer C.
  TITLE     LucTrap vectors are tools to generate luciferase fusions for the 
            quantification of transcript and protein abundance in vivo
  JOURNAL   Plant Physiol. 141 (1), 3-14 (2006)
  PUBMED    16684932
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Calderon LIA. V, Kuhnle C, Dohmann EMN., Li H, Schwechheimer C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2005) Developmental Genetics, Centre for Plant 
            Molecular Biology, Auf der Morgenstelle 5, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 7272)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006"; volume: "141"; issue: "1"; pages: "3-14"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2005) Developmental Genetics, Centre for Plant Molecular 
            Biology, Auf der Morgenstelle 5, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             320..1132
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1236..1824
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1915..1939)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    1950..2074
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2099..2129
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2777..3079
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3123..3247)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3252..4901
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5059..5075)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5101..5119)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5126..5142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5389..5568
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5602..6393
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      6436..6687
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(6711..6733)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(join(6866..7272,1..29))
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 agcccgctcg acggcgggct gttggatggg gatcgcctga atcgccccat catccagcca
       61 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
      121 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
      181 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg
      241 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      301 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg
      361 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      421 gcaatcaggt gcgacaatct atggattgta tgggaagccc gatgcgccag agttgtttct
      481 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      541 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      601 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
      661 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      721 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      781 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
      841 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
      901 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      961 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1021 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1081 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1141 attggttaat tggttgtaac catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgagatct caaacaaaca catacagcga cttagtttac
     1921 ccgccaatat atcctgtcaa ggcctatcaa caagtttgta caaaaaagct gaacgagaaa
     1981 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     2041 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     2101 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt
     2161 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     2221 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     2281 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     2341 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctt aatgaattac
     2401 aacagtactg cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag
     2461 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     2521 tgtatacccg aagtatgtca aaaagaggtg tgctatgaag cagcgtatta cagtgacagt
     2581 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     2641 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     2701 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     2761 aacagggact ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     2821 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     2881 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     2941 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     3001 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     3061 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     3121 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     3181 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     3241 aagtggttgc catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
     3301 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
     3361 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact
     3421 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
     3481 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
     3541 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
     3601 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt
     3661 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg
     3721 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     3781 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga
     3841 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg
     3901 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     3961 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     4021 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc
     4081 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca
     4141 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg
     4201 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca
     4261 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     4321 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
     4381 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga
     4441 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
     4501 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc
     4561 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca
     4621 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg
     4681 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     4741 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     4801 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     4861 agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag atcactggat
     4921 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     4981 tttcttatat gctcaacaca tgagcgaaac cctataagaa ccctaattcc cttatctgga
     5041 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     5101 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5161 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5221 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5281 ggcgcgaaat tgtaaacgtt aatgttatcg atacatgaga attaagggag tcacgttatg
     5341 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
     5401 tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
     5461 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
     5521 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
     5581 cactctcaac tcgatcgagg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5641 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5701 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5761 tccggtgccc tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg
     5821 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5881 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5941 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6001 gaccaccaag cgaaacatcg catcgagcga ggacgtactc ggatggaagc cggtcttgtc
     6061 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6121 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccagggcga tgcctgcttg
     6181 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6241 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6301 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6361 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggactct
     6421 agctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     6481 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     6541 attaacatgt aatgcatgac gttatttatg agatgggttt tttgattaga gtcccgcaat
     6601 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     6661 gcgcggtgtc atctatgtta ctagatcgac cggcatgcaa gctgataacg ttacaccaca
     6721 atatatcctg ccaagatcta attccgggga tcggaaatcc agaagcccga gaggttgccg
     6781 cctttcgggc tttttctttt tcaaaaaaaa aaatttataa aacgatctgt tgcggccggc
     6841 cgccgggttg tgggcaaagg cgctggcgct cgacggtggg caaccgcttg cggttgtcca
     6901 cgggcggagc cggtgcgcgt agcgcattgt ccacaagcca agggcgacca ataattgata
     6961 tatatattca taattgaaaa gctaattgaa catactactt gctgtaacta cttgccggag
     7021 cgaggggtgt ttgcaagctg ttgatctgaa agggctatta gcgttctcac gtgccttttt
     7081 gattagcgat ttcacgtgac cttattagcg atttcacgta ctccgattag cgatttcacg
     7141 taccctgatt agcgatttca cgtggatagt ttttggagcg ggccggaaag ccccgtgaat
     7201 caaggctttg cggggcatta gcggtttcac gtggataact accctctatc cacaggcttc
     7261 cggggataaa aa
//
