LOCUS       40924_1839        7493 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector LNXCO4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7493)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             146..733
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    796..995
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1196..1612
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     CDS             1656..2447
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    complement(3033..3080)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    3470..3489
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3513..3532
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3556..3575
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    complement(3743..3762)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(3768..3969)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3970..4349)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             4539..5132
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    5343..5483
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5669..6257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6431..7288)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7289..7393)
                     /label=AmpR promoter
ORIGIN
        1 gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
       61 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
      121 ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
      181 aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
      241 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
      301 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
      361 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
      421 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
      481 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
      541 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
      601 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
      661 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
      721 ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
      781 ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
      841 tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
      901 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
      961 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     1021 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     1081 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     1141 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     1201 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     1261 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     1321 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     1381 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     1441 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     1501 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     1561 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
     1621 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     1681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     1801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     2461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     2521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     2581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
     2641 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
     2701 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
     2761 ggagtgggga ggcacgatgg ccgctttggt cgaggcggat cctagccccc acgaaccata
     2821 aaccattccc catgggggac cccgtcccta acccacgggg ccagtggcta tggcagggcc
     2881 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     2941 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     3001 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     3061 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     3121 ttcagttctt aagaattgcg gccgcgatcg cgttaacaga tctggccgca agcttggttg
     3181 gaatctaaaa tacacaaaca attaggatcc ctgaggagca gttctttgat ttgcaccacc
     3241 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
     3301 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
     3361 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
     3421 gggagacgga tccgtagttt aacacattat acactagggt cgactgtgga attgtgagcg
     3481 ctcacaattc cacaacctag ggtcgactgt ggaattgtga gcgctcacaa ttccacaacc
     3541 tagggtcgac tgtggaattg tgagcgctca caattccaca acctagtaaa ttttatattt
     3601 accttagagc tttaaatctc tgtaggtagt ttgtccaagc ttggggccgc ggaggctgga
     3661 tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
     3721 acggttcact aaacgagtgt ggaattgtga gcgctcacaa ttccacactc tgcttatata
     3781 gacctcccac cgtacatgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     3841 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     3901 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     3961 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     4021 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     4081 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     4141 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     4201 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     4261 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     4321 ctattaataa ctagtcaata atcaatgtca acatggcggt aatgttggac atgagccaat
     4381 ataaatgtac atattatgat atggatacaa cgtatgcaat ggccaatagc caatattgat
     4441 ttatgctata taaccaatga ataatatggc taatggccgg atccccggtg aacatcgata
     4501 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     4561 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     4621 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     4681 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     4741 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     4801 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     4861 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     4921 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     4981 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     5041 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     5101 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc
     5161 ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt
     5221 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5281 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5341 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     5401 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     5461 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5521 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6241 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6361 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6481 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6541 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6601 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6661 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6721 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6781 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6901 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6961 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7021 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     7081 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7141 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7201 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7261 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7321 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7381 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     7441 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caa
//
