LOCUS       .                       9776 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1684..1917
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2102..2146
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2295..2336
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2444..2561
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    2874..3008
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             3533..5182
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    5223..5809
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5810..6526
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     protein_bind    complement(6576..6609)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6665..7253
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(7256..7272)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(8024..8612)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8786..9643)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9644..9748)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagac cgggtcatgg ctgcgccccg
     2641 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2701 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2761 gaaacgcgcg aggcaggatc agccatatca catttgtaga ggttttactt gctttaaaaa
     2821 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttaacttgt
     2881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3001 tctggatcag ccatatcaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3061 tccccctgaa cctgaaacat aaaatgaatg caattgttgt taacttgttt attgcagctt
     3121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccaag
     3241 ctcctgcctg gaagatctcg agatctgcaa ggatgagtca agctgcgggt gatccagatc
     3301 tgcaaggatg agtcaagctg cgggtgatcc agatctgcaa ggatgagtca agctgcgggt
     3361 gatccagatc tgggcccgga tcctgcaggg ggtcgacaag cttcgcgagg tcaccattaa
     3421 tcatttcctc tgtgtattta agagctcttt tgccagtgag cccagtacac agagagaaag
     3481 gctaaagttc tgtcgacaag cttggcattc cggtactgtt ggtaaagcca ccatggaaga
     3541 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     3601 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     3661 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     3721 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     3781 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     3841 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     3901 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     3961 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4021 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     4081 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     4141 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     4201 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4261 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4321 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     4381 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     4441 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     4501 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     4561 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     4621 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     4681 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     4741 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     4801 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     4861 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     4921 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     4981 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5041 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5101 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5161 gaagggcgga aagatcgccg tgtaattcta gggcggccgc tacgtaaatt ccgccccccc
     5221 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5281 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5341 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5401 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5461 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5521 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5581 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5641 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5701 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5761 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
     5821 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5881 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5941 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6001 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6061 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6121 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6181 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6241 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6301 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6361 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6421 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6481 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccggcc
     6541 gccagcacag tggtcgaaat tcgtcgaggg acctaataac ttcgtatagc atacattata
     6601 cgaagttata catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat
     6661 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6721 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6781 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6841 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6901 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6961 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7021 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7081 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7141 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7201 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     7261 ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7321 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7381 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7501 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7561 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7621 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     7681 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     7741 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     7801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7921 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc
     7981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8581 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8641 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8701 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8761 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8821 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8881 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8941 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9001 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9061 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9121 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9181 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9241 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9301 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9361 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9421 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9481 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9541 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9601 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9661 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9721 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
