LOCUS       .                      10961 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4241..4957
                     /label="ECFP"
                     /note="enhanced CFP"
     CDS             4964..6475
                     /gene="YAP1"
                     /label="YAP1"
                     /note="Transcriptional coactivator YAP1 from Homo sapiens.
                     Accession#: P46937"
     polyA_signal    6528..6752
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6798..7226
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7240..7569
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7636..8427
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    8604..8737
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8822..8852)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8867..8888)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9176..9761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9935..10792)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(10793..10897)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgag
      901 gccaaagact tttcctgcca cgacttattc tggaaatagc cggcagcgac tgcaagagat
      961 tcgtgagggg ttaaaacagc catccaagtc ttcggttcag gggctacccg caggaccaaa
     1021 cagtgacact tccctggatg ccaaagtcct ggggagcaaa gatgccacca ggcagcagca
     1081 gcagatgaga gccaccccaa agttcggacc ttatcagaaa gccttgaggg aaatcagata
     1141 ttccttgttg ccttttgcta atgaatcggg cacctctgca gctgcagaag tgaaccggca
     1201 aatgctgcag gaactggtga acgcaggatg cgaccaggag atggctggcc gagctctcaa
     1261 gcagactggc agcaggagca tcgaggccgc cctggagtac atcagcaaga tgggctacct
     1321 ggacccgagg aatgagcaga ttgtgcgggt cattaagcag acctccccag gaaaggggct
     1381 catgccaacc ccagtgacgc ggaggcccag cttcgaagga accggcgatt cgtttgcgtc
     1441 ctaccaccag ctgagcggta ccccctacga gggcccaagc ttcggcgctg acggccccac
     1501 ggcgctggag gagatgccgc ggccgtacgt ggactacctt ttccccggag tcggccccca
     1561 cgggcccggc caccagcacc agcacccacc caagggctac ggtgccagcg tagaggcagc
     1621 aggggcacac ttcccgctgc agggcgcgca ctacgggcgg ccgcacctgc tggtgcctgg
     1681 ggaacccctg ggctacggag tgcagcgcag cccctccttc cagagcaaga cgccgccgga
     1741 gaccgggggt tacgccagcc tgcccacgaa gggccaggga ggaccgccag gcgccggcct
     1801 cgctttccca ccccctgccg ccgggctcta cgtgccgcac ccacaccaca agcaggccgg
     1861 tcccgcggcc caccagctgc atgtgctggg ctcccgcagc caggtgttcg ccagcgacag
     1921 ccccccgcag agcctgctca ctccctcgcg gaacagcctc aacgtggacc tgtatgaatt
     1981 gggcagcacc tccgtccagc agtggccggc tgccaccctg gcccgccggg actccctgca
     2041 gaagccgggc ctggaggcgc cgccgcgcgc gcacgtggcc ttccggcctg actgcccagt
     2101 gcccagcagg accaactcct tcaacagcca ccagccgcgg cccggtccgc ctggcaaggc
     2161 cgagccctcc ctgcccgccc ccaacaccgt gacggctgtc acggccgcgc acatcttgca
     2221 cccggtgaag agcgtgcgtg tgctgaggcc ggagccgcag acggctgtgg ggccctcgca
     2281 ccccgcctgg gtgcccgcgc ctgccccggc ccccgccccc gcccccgccc cggctgcgga
     2341 gggcttggac gccaaggagg agcatgccct ggcgctgggc ggcgcaggcg ccttcccgct
     2401 ggacgtggag tacggaggcc cagaccggag gtgcccgcct ccgccctacc cgaagcacct
     2461 gctgctgcgc agcaagtcgg agcagtacga cctggacagc ctgtgcgcag gcatggagca
     2521 gagcctccgt gcgggcccca acgagcccga gggcggcgac aagagccgca aaagcgccaa
     2581 gggggacaaa ggcggaaagg ataaaaagca gattcagacc tctcccgttc ccgtccgcaa
     2641 aaacagcaga gacgaagaga agagagagtc acgcatcaag agctactcgc catacgcctt
     2701 taagttcttc atggagcagc acgtggagaa tgtcatcaaa acctaccagc agaaggttaa
     2761 ccggaggctg cagctggagc aagaaatggc caaagctgga ctctgtgaag ctgagcagga
     2821 gcagatgcgg aagatcctct accagaaaga gtctaattac aacaggttaa agagggccaa
     2881 gatggacaag tctatgtttg tcaagatcaa aaccctgggg atcggtgcct ttggagaagt
     2941 gtgccttgct tgtaaggtgg acactcacgc cctgtacgcc atgaagaccc taaggaaaaa
     3001 ggatgtcctg aaccggaatc aggtggccca cgtcaaggcc gagagggaca tcctggccga
     3061 ggcagacaat gagtgggtgg tcaaactcta ctactccttc caagacaaag acagcctgta
     3121 ctttgtgatg gactacatcc ctggtgggga catgatgagc ctgctgatcc ggatggaggt
     3181 cttccctgag cacctggccc ggttctacat cgcagagctg actttggcca ttgagagtgt
     3241 ccacaagatg ggcttcatcc accgagacat caagcctgat aacattttga tagatctgga
     3301 tggtcacatt aaactcacag atttcggcct ctgcactggg ttcaggtgga ctcacaattc
     3361 caaatattac cagaaaggga gccatgtcag acaggacagc atggagccca gcgacctctg
     3421 ggatgatgtg tctaactgtc ggtgtgggga caggctgaag accctagagc agagggcgcg
     3481 gaagcagcac cagaggtgcc tggcacattc actggtgggg actccaaact acatcgcacc
     3541 cgaggtgctc ctccgcaaag ggtacactca actctgtgac tggtggagtg ttggagtgat
     3601 tctcttcgag atgctggtgg ggcagccgcc ctttttggca cctactccca cagaaaccca
     3661 gctgaaggtg atcaactggg agaacacgct ccacattcca gcccaggtga agctgagccc
     3721 tgaggccagg gacctcatca ccaagctgtg ctgctccgca gaccaccgcc tggggcggaa
     3781 tggggccgat gacctgaagg cccacccctt cttcagcgcc attgacttct ccagtgacat
     3841 ccggaagcag ccagccccct acgttcccac catcagccac cccatggaca cctcgaattt
     3901 cgaccccgta gatgaagaaa gcccttggaa cgatgccagc gaaggtagca ccaaggcctg
     3961 ggacacactc acctcgccca ataacaagca tcctgagcac gcattttacg aattcacctt
     4021 ccgaaggttc tttgatgaca atggctaccc ctttcgatgc ccaaagcctt caggagcaga
     4081 agcttcacag gctgagagct cagatttaga aagctctgat ctggtggatc agactgaagg
     4141 ctgccagcct gtgtacgtgg gatccggaag cggagccact aacttctccc tgttgaaaca
     4201 agcaggggat gtcgaagaga atcccgggcc aggtggatca atggtgagca agggcgagga
     4261 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4321 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4381 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg
     4441 gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4501 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4561 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4621 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat
     4681 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     4741 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4801 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4861 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4921 cgccgggatc actctcggca tggacgagct gtacaaggaa ttcatggatc ccgggcagca
     4981 gccgccgcct caaccggccc cccagggcca agggcagccg ccttcgcagc ccccgcaggg
     5041 gcagggcccg ccgtccggac ccgggcaacc ggcacccgcg gcgacccagg cggcgccgca
     5101 ggcacccccc gccgggcatc agatcgtgca cgtccgcggg gactcggaga ccgacctgga
     5161 ggcgctcttc aacgccgtca tgaaccccaa gacggccaac gtgccccaga ccgtgcccat
     5221 gaggctccgg aagctgcccg actccttctt caagccgccg gagcccaaat cccactcccg
     5281 acaggccagt actgatgcag gcactgcagg agccctgact ccacagcatg ttcgagctca
     5341 ttcctctcca gcttctctgc agttgggagc tgtttctcct gggacactga cccccactgg
     5401 agtagtctct ggcccagcag ctacacccac agctcagcat cttcgacagt cttcttttga
     5461 gatacctgat gatgtacctc tgccagcagg ttgggagatg gcaaagacat cttctggtca
     5521 gagatacttc ttaaatcaca tcgatcagac aacaacatgg caggacccca ggaaggccat
     5581 gctgtcccag atgaacgtca cagcccccac cagtccacca gtgcagcaga atatgatgaa
     5641 ctcggcttca ggtcctcttc ctgatggatg ggaacaagcc atgactcagg atggagaaat
     5701 ttactatata aaccataaga acaagaccac ctcttggcta gacccaaggc ttgaccctcg
     5761 ttttgccatg aaccagagaa tcagtcagag tgctccagtg aaacagccac cacccctggc
     5821 tccccagagc ccacagggag gcgtcatggg tggcagcaac tccaaccagc agcaacagat
     5881 gcgactgcag caactgcaga tggagaagga gaggctgcgg ctgaaacagc aagaactgct
     5941 tcggcaggca atgcggaata tcaatcccag cacagcaaat tctccaaaat gtcaggagtt
     6001 agccctgcgt agccagttac caacactgga gcaggatggt gggactcaaa atccagtgtc
     6061 ttctcccggg atgtctcagg aattgagaac aatgacgacc aatagctcag atcctttcct
     6121 taacagtggc acctatcact ctcgagatga gagtacagac agtggactaa gcatgagcag
     6181 ctacagtgtc cctcgaaccc cagatgactt cctgaacagt gtggatgaga tggatacagg
     6241 tgatactatc aaccaaagca ccctgccctc acagcagaac cgtttcccag actaccttga
     6301 agccattcct gggacaaatg tggaccttgg aacactggaa ggagatggaa tgaacataga
     6361 aggagaggag ctgatgccaa gtctgcagga agctttgagt tctgacatcc ttaatgacat
     6421 ggagtctgtt ttggctgcca ccaagctaga taaagaaagc tttcttacat ggttaaccgg
     6481 ttagctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6541 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6601 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6661 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6721 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6781 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6841 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6901 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6961 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7021 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7081 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7141 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7201 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7261 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7321 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7381 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7441 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7501 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7561 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     7621 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     7681 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     7741 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     7801 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     7861 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     7921 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     7981 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     8041 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     8101 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     8161 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     8221 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     8281 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     8341 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     8401 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     8461 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     8521 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8581 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     8641 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8701 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8761 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8821 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8881 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8941 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9001 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9061 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9121 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9181 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9241 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9361 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9421 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9481 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9541 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9601 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     9721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10921 ctgacgtcga cggatcggga gatctcccga tcccctatgg t
//
