LOCUS       40924_1334        8356 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector HPdO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva 
            L, Liu DR, Chen IA.
  TITLE     Inhibition of Bacterial Conjugation by Phage M13 and Its Protein 
            g3p: Quantitative Analysis and Model
  JOURNAL   PLoS ONE 6 (5), E19991 (2011)
  PUBMED    21637841
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Esvelt KM, Carlson JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8356)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8356)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "5"; pages: "E19991"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-OCT-2010) Chemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1230
                     /codon_start=1
                     /label=M13 gene II
                     /note="pII"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHVDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             1245..1505
                     /codon_start=1
                     /label=M13 gene V
                     /note="pV"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1510..1608
                     /codon_start=1
                     /label=M13 gene VII
                     /note="pVII"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1608..1703
                     /codon_start=1
                     /label=M13 gene IX
                     /note="pIX"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             1703..1921
                     /codon_start=1
                     /label=M13 gene VIII
                     /note="pVIII"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      1928..1976
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             1981..3252
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="VKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             3258..3593
                     /codon_start=1
                     /label=M13 gene VI
                     /note="pVI"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             3598..4641
                     /codon_start=1
                     /label=M13 gene I
                     /note="pI"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             4622..5899
                     /codon_start=1
                     /label=M13 gene IV
                     /note="pIV"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     rep_origin      complement(6135..6680)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(7232..8044)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc
       61 tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg
      121 aatttatcag ctagaacggt tgaatatcat gttgatggtg atttgactgt ctccggcctt
      181 tctcaccctt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag
      241 ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag
      301 ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat
      361 tttgctaatt ctttgccttg cctgtatgat ttattggatg ttaacgctac tactattagt
      421 agaattgatg ccaccttttc agctcgcgcc ccaaatgaaa atatagctaa acaggttatt
      481 gaccatttgc gaaatgtatc taatggtcaa actaaatcta ctcgttcgca gaattgggaa
      541 tcaactgtta catggaatga aacttccaga caccgtactt tagttgcata tttaaaacat
      601 gttgagctac agcaccagat tcagcaatta agctctaagc catccgcaaa aatgacctct
      661 tatcaaaagg agcaattaaa ggtactctct aatcctgacc tgttggagtt tgcttccggt
      721 ctggttcgct ttgaagctcg aattaaaacg cgatatttga agtctttcgg gcttcctctt
      781 aatctttttg atgcaatccg ctttgcttct gactataata gtcagggtaa agacctgatt
      841 tttgatttat ggtcattctc gttttctgaa ctgtttaaag catttgaggg ggattcaatg
      901 aatatttatg acgattccgc agtattggac gctatccagt ctaaacattt tactattacc
      961 ccctctggca aaacttcttt tgcaaaagcc tctcgctatt ttggttttta tcgtcgtctg
     1021 gtaaacgagg gttatgatag tgttgctctt actatgcctc gtaattcctt ttggcgttat
     1081 gtatctgcat tagttgaatg tggtattcct aaatctcaac tgatgaatct ttctacctgt
     1141 aataatgttg ttccgttagt tcgttttatt aacgtagatt tttcttccca acgtcctgac
     1201 tggtataatg agccagttct taaaatcgca taaggtaatt cacaatgatt aaagttgaaa
     1261 ttaaaccatc tcaagcccaa tttactactc gttctggtgt ttctcgtcag ggcaagcctt
     1321 attcactgaa tgagcagctt tgttacgttg atttgggtaa tgaatatccg gttcttgtca
     1381 agattactct tgatgaaggt cagccagcct atgcgcctgg tctgtacacc gttcatctgt
     1441 cctctttcaa agttggtcag ttcggttccc ttatgattga ccgtctgcgc ctcgttccgg
     1501 ctaagtaaca tggagcaggt cgcggatttc gacacaattt atcaggcgat gatacaaatc
     1561 tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtcaaagatg agtgttttag
     1621 tgtattcttt cgcctctttc gttttaggtt ggtgccttcg tagtggcatt acgtatttta
     1681 cccgtttaat ggaaacttcc tcatgaaaaa gtctttagtc ctcaaagcct ctgtagccgt
     1741 tgctaccctc gttccgatgc tgtctttcgc tgctgagggt gacgatcccg caaaagcggc
     1801 ctttaactcc ctgcaagcct cagcgaccga atatatcggt tatgcgtggg cgatggttgt
     1861 tgtcattgtc ggcgcaacta tcggtatcaa gctgtttaag aaattcacct cgaaagcaag
     1921 ctgataaacc gatacaatta aaggctcctt ttggagcctt tttttttgga gattttcaac
     1981 gtgaaaaaat tattattcgc aattccttta gttgttcctt tctattctca ctccgctgaa
     2041 actgttgaaa gttgtttagc aaaaccccat acagaaaatt catttactaa cgtctggaaa
     2101 gacgacaaaa ctttagatcg ttacgctaac tatgagggct gtctgtggaa tgctacaggc
     2161 gttgtagttt gtactggtga cgaaactcag tgttacggta catgggttcc tattgggctt
     2221 gctatccctg aaaatgaggg tggtggctct gagggtggcg gttctgaggg tggcggttct
     2281 gagggtggcg gtactaaacc tcctgagtac ggtgatacac ctattccggg ctatacttat
     2341 atcaaccctc tcgacggcac ttatccgcct ggtactgagc aaaaccccgc taatcctaat
     2401 ccttctcttg aggagtctca gcctcttaat actttcatgt ttcagaataa taggttccga
     2461 aataggcagg gggcattaac tgtttatacg ggcactgtta ctcaaggcac tgaccccgtt
     2521 aaaacttatt accagtacac tcctgtatca tcaaaagcca tgtatgacgc ttactggaac
     2581 ggtaaattca gagactgcgc tttccattct ggctttaatg aggatccatt cgtttgtgaa
     2641 tatcaaggcc aatcgtctga cctgcctcaa cctcctgtca atgctggcgg cggctctggt
     2701 ggtggttctg gtggcggctc tgagggtggt ggctctgagg gtggcggttc tgagggtggc
     2761 ggctctgagg gaggcggttc cggtggtggc tctggttccg gtgattttga ttatgaaaag
     2821 atggcaaacg ctaataaggg ggctatgacc gaaaatgccg atgaaaacgc gctacagtct
     2881 gacgctaaag gcaaacttga ttctgtcgct actgattacg gtgctgctat cgatggtttc
     2941 attggtgacg tttccggcct tgctaatggt aatggtgcta ctggtgattt tgctggctct
     3001 aattcccaaa tggctcaagt cggtgacggt gataattcac ctttaatgaa taatttccgt
     3061 caatatttac cttccctccc tcaatcggtt gaatgtcgcc cttttgtctt tggcgctggt
     3121 aaaccatatg aattttctat tgattgtgac aaaataaact tattccgtgg tgtctttgcg
     3181 tttcttttat atgttgccac ctttatgtat gtattttcta cgtttgctaa catactgcgt
     3241 aataaggagt cttaatcatg ccagttcttt tgggtattcc gttattattg cgtttcctcg
     3301 gtttccttct ggtaactttg ttcggctatc tgcttacttt tcttaaaaag ggcttcggta
     3361 agatagctat tgctatttca ttgtttcttg ctcttattat tgggcttaac tcaattcttg
     3421 tgggttatct ctctgatatt agcgctcaat taccctctga ctttgttcag ggtgttcagt
     3481 taattctccc gtctaatgcg cttccctgtt tttatgttat tctctctgta aaggctgcta
     3541 ttttcatttt tgacgttaaa caaaaaatcg tttcttattt ggattgggat aaataatatg
     3601 gctgtttatt ttgtaactgg caaattaggc tctggaaaga cgctcgttag cgttggtaag
     3661 attcaggata aaattgtagc tgggtgcaaa atagcaacta atcttgattt aaggcttcaa
     3721 aacctcccgc aagtcgggag gttcgctaaa acgcctcgcg ttcttagaat accggataag
     3781 ccttctatat ctgatttgct tgctattggg cgcggtaatg attcctacga tgaaaataaa
     3841 aacggcttgc ttgttctcga tgagtgcggt acttggttta atacccgttc ttggaatgat
     3901 aaggaaagac agccgattat tgattggttt ctacatgctc gtaaattagg atgggatatt
     3961 atttttcttg ttcaggactt atctattgtt gataaacagg cgcgttctgc attagctgaa
     4021 catgttgttt attgtcgtcg tctggacaga attactttac cttttgtcgg tactttatat
     4081 tctcttatta ctggctcgaa aatgcctctg cctaaattac atgttggcgt tgttaaatat
     4141 ggcgattctc aattaagccc tactgttgag cgttggcttt atactggtaa gaatttgtat
     4201 aacgcatatg atactaaaca ggctttttct agtaattatg attccggtgt ttattcttat
     4261 ttaacgcctt atttatcaca cggtcggtat ttcaaaccat taaatttagg tcagaagatg
     4321 aaattaacta aaatatattt gaaaaagttt tctcgcgttc tttgtcttgc gattggattt
     4381 gcatcagcat ttacatatag ttatataacc caacctaagc cggaggttaa aaaggtagtc
     4441 tctcagacct atgattttga taaattcact attgactctt ctcagcgtct taatctaagc
     4501 tatcgctatg ttttcaagga ttctaaggga aaattaatta atagcgacga tttacagaag
     4561 caaggttatt cactcacata tattgattta tgtactgttt ccattaaaaa aggtaattca
     4621 aatgaaattg ttaaatgtaa ttaattttgt tttcttgatg tttgtttcat catcttcttt
     4681 tgctcaggta attgaaatga ataattcgcc tctgcgcgat tttgtaactt ggtattcaaa
     4741 gcaatcaggc gaatccgtta ttgtttctcc cgatgtaaaa ggtactgtta ctgtatattc
     4801 atctgacgtt aaacctgaaa atctacgcaa tttctttatt tctgttttac gtgcaaataa
     4861 ttttgatatg gtaggttcta acccttccat tattcagaag tataatccaa acaatcagga
     4921 ttatattgat gaattgccat catctgataa tcaggaatat gatgataatt ccgctccttc
     4981 tggtggtttc tttgttccgc aaaatgataa tgttactcaa acttttaaaa ttaataacgt
     5041 tcgggcaaag gatttaatac gagttgtcga attgtttgta aagtctaata cttctaaatc
     5101 ctcaaatgta ttatctattg acggctctaa tctattagtt gttagtgctc ctaaagatat
     5161 tttagataac cttcctcaat tcctttcaac tgttgatttg ccaactgacc agatattgat
     5221 tgagggtttg atatttgagg ttcagcaagg tgatgcttta gatttttcat ttgctgctgg
     5281 ctctcagcgt ggcactgttg caggcggtgt taatactgac cgcctcacct ctgttttatc
     5341 ttctgctggt ggttcgttcg gtatttttaa tggcgatgtt ttagggctat cagttcgcgc
     5401 attaaagact aatagccatt caaaaatatt gtctgtgcca cgtattctta cgctttcagg
     5461 tcagaagggt tctatctctg ttggccagaa tgtccctttt attactggtc gtgtgactgg
     5521 tgaatctgcc aatgtaaata atccatttca gacgattgag cgtcaaaatg taggtatttc
     5581 catgagcgtt tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa
     5641 ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat
     5701 tgctacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta
     5761 taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct
     5821 cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc
     5881 aaccatagta cgcgccctgt agcggcgcat taagcgctag cagtgagctt catgtggcag
     5941 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     6001 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     6061 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     6121 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     6181 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     6241 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     6301 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     6361 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     6421 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     6481 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     6541 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     6601 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     6661 gcagaccaaa acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg
     6721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7021 taaaccagcc agccgattcg agctcgcccg gggatcgacc agttggtgat tttgaacttt
     7081 tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca
     7141 gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc
     7201 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     7261 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     7321 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     7381 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     7441 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     7501 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     7561 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     7621 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     7681 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     7741 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     7801 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     7861 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     7921 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     7981 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     8041 tcataacccc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     8101 tatttttatc ttgtgcaatg taacatcaga gattttgaaa cacaacgtgg ctttcccccc
     8161 cccccccctg caggtctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     8221 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8281 aacgtttaca atttaaatat ttgcttatac aatcttcctg tttttggggc ttttcttatt
     8341 atcaaccggg gtacat
//
