LOCUS       .                       6881 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(45..185)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(290..478)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        974..1051
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1052..2131
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    2147..2168
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2377..2405
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    2413..2429
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             2502..2519
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2520..3620
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             3627..3650
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     primer_bind     complement(3945..3961)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      4310..4396
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4488..4515
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        4534..4625
                     /label="AmpR promoter"
     CDS             4626..5483
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      complement(5528..6041)
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      6152..6740
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
       61 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      121 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
      181 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      241 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      301 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg
      361 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
      421 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
      481 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
      541 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
      601 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
      661 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
      721 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
      781 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
      841 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
      901 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc
      961 tgcccgaaat tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg
     1021 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
     1081 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     1141 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     1201 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     1261 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     1321 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     1381 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
     1441 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
     1501 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
     1561 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
     1621 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
     1681 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
     1741 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
     1801 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
     1861 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     1921 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     1981 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     2041 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2101 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc
     2161 tcactcatta ggcacaattc tcatgtttga cagcttatca tcgactgcac ggtgcaccaa
     2221 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
     2281 tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
     2341 tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca tcggctcgta
     2401 taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagccag tccgtttagg
     2461 tgttttcacg agcacttcac caacaaggac catagcatat gcatcaccat caccatcaca
     2521 tgaaaactga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc
     2581 tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc
     2641 cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta
     2701 tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca
     2761 ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca
     2821 acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag
     2881 atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga
     2941 aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc
     3001 tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag
     3061 acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta
     3121 aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag
     3181 gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag
     3241 tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg
     3301 gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc
     3361 tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg
     3421 gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca
     3481 ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct
     3541 ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag
     3601 ccctgaaaga cgcgcagact gaattcctgg aagttctgtt ccaggggccc ggatccactc
     3661 tctacagtga ccaggagctg gcctatctcc agcaggggga ggaggccatg cagaaggcct
     3721 tgggcatcct tagcaaccaa gagggctgga agaaggagag tcagcaggac aatggggaca
     3781 aagtgatgag taaagtggtc ccagatgtgg gcaaggtgtt ccggctggag gtcgtggtgg
     3841 accagcccat ggagaggctc tatgaagagc tcgtggagcg catggaagca atgggggagt
     3901 ggaaccccaa tgtcaaggag atcaaggtcc tgcagaagct tggcactggc cgtcgtttta
     3961 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4021 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4081 cgcagcctga atggcgaatg gcagcttggc tgttttggcg gatgagataa gattttcagc
     4141 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
     4201 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     4261 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     4321 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     4381 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     4441 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     4501 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     4561 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4621 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4681 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4741 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4801 gccccgaaga acgtttccca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4861 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4921 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4981 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5041 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5101 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5161 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5221 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5281 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5341 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5401 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5461 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5521 ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg tataagcaaa
     5581 tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5641 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagcc
     5701 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     5761 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     5821 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     5881 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     5941 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6001 caccacaccc gccgcgctta atgcgccgct acagggcgcg taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg a
//
