LOCUS       40924_1154        5111 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM4-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5111)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      411..1828
                     /label=GmScreamM4 promoter
                     /note="GmScreamM4 promoter"
                     /regulatory_class="promoter"
     CDS             1831..2547
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2583..2835
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2890..2906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2914..2930)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2938..2968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2983..3004)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3292..3880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4054..4911)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4912..5016)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag cctgaacttg
      421 tattattcag tagatcgaat aaattataaa aagataaaat cataaaataa tattttatcc
      481 tatcaatcat attaaagcaa tgaatatgta aaattaatct tatctttatt ttaaaaaatc
      541 atataggttt agtatttttt taaaaataaa gataggatta gttttactat tcactgctta
      601 ttacttttaa aaaaatcata aaggtttagt atttttttaa aataaatata ggaatagttt
      661 tactattcac tgctttaata gaaaaatagt ttaaaattta agatagtttt aatcccagca
      721 tttgccacgt ttgaacgtga gccgaaacga tgtcgttaca ttatcttaac ctagctgaaa
      781 cgatgtcgtc ataatatcgc caaatgccaa ctggactacg tcgaacccac aaatcccaca
      841 aagcgcgtga aatcaaatcg ctcaaaccac aaaaaagaac aacgcgtttg ttacacgctc
      901 aatcccacgc gagtagagca cagtaacctt caaataagcg aatggggcat aatcagaaat
      961 ccgaaataaa cctaggggca ttatcggaaa tgaaaagtag ctcactcaat ataaaaatct
     1021 aggaacccta gttttcgtta tcactctgtg ctccctcgct ctatttctca gtctctgtgt
     1081 ttgcggctga ggattccgaa cgagtgacct tcttcgtttc tcgcaaaggt aacagcctct
     1141 gctcttgtct cttcgattcg atctatgcct gtctcttatt tacgatgatg tttcttcggt
     1201 tatgtttttt tatttatgct ttatgctgtt gatgttcggt tgtttgtttc gctttgtttt
     1261 tgtggttcag ttttttagga ttcttttggt ttttgaatcg attaatcgga agagattttc
     1321 gagttatttg gtgtgttgga ggtgaatctt ttttttgagg tcatagatct gttgtatttg
     1381 tgttataaac atgcgacttt gtatgatttt ttacgaggtt atgatgttct ggttgtttta
     1441 ttatgaatct gttgagacag aaccatgatt tttgttgatg ttcgtttaca ctattaaagg
     1501 tttgttttaa caggattaaa agttttttaa gcatgttgaa ggagtcttgt agatatgtaa
     1561 ccgtcgatag tttttttgtg ggtttgttca catgttatca agcttaatct tttactatgt
     1621 atgcgaccat atctggatcc agcaaaggcg attttttaat tccttgtgaa acttttgtaa
     1681 tatgaagttg aaattttgtt attggtaaac tataaatgtg tgaagttgga gtataccttt
     1741 accttcttat ttggctttgt gatagtttaa tttatatgta ttttgagttc tgacttgtat
     1801 ttctttgaat tgattctagt ttaagtaacc atggtgagca agggcgagga gctgttcacc
     1861 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1921 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1981 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag
     2041 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2101 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2161 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2221 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2281 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2341 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2401 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2461 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2521 actcacggca tggacgagct gtacaagtaa agcggccgcc cggctgcact gaggcctcag
     2581 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     2641 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     2701 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     2761 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     2821 ctatgttact agatccgatg ataagctgtc aaacatgaga attagatctc aattgaattc
     2881 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2941 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3001 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3061 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3721 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4021 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4081 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4141 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4201 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4261 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4321 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4381 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4441 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4501 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4561 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4621 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4681 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4741 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4801 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4861 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4921 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4981 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5041 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5101 gccctttcgt c
//
