LOCUS       40924_1144        5218 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM2-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5218)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5218)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      411..1935
                     /label=GmScreamM2 promoter
                     /note="GmScreamM2 promoter"
                     /regulatory_class="promoter"
     CDS             1938..2654
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2690..2942
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2997..3013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3021..3037)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3045..3075)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3090..3111)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3399..3987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4161..5018)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5019..5123)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag aggaactgtc
      421 atgtcctcac attaatgaat tctctatttt tatttttttt taatttagta gcacgtaaga
      481 ttataaaagt cacaaaaaac tcaagtgtta ttattaggag agtaaaaaat gatatacaaa
      541 tatcataaaa ataactaatt aaaaaataat tactattatg taaaatcatt aaaaaaataa
      601 aattactcgt aagagatata ttacaaaaaa ttattaattt aacttataca gtatgaactt
      661 aattatgttc acctttattg atgaagttta ttcactgtta ttgtcatttg tgtgataaga
      721 gggaagaaga agagatgaat caagttgagt cagaggcagc agatgaaacc attcaagggg
      781 tagtaacaga cacaacacag gtgcaagaag aagcagagaa aaccaaaaga gtagtgagga
      841 agcccactta cttcaaggac tttgtctaaa gggtgaagtg tgtatttcca taacaaaatc
      901 atcaacaaac aaattctgtt actaggatta ctataaatac aattgtaata aacaagcaaa
      961 gatatgaatg aatgaaattt ccatatttct tacttttctt ctttggaggg ttctggtcct
     1021 cgaaaccaaa ttcttagttc tcttaccaat ttgtctcttt ttggttggtt attcagtgta
     1081 acaaaataaa aaaaataaga attatgaatc taaataatgg tatacatgac aaagagatat
     1141 agctcacatt gacaactaaa aattgaatta ctgatcactt gcattttcat ttatctttta
     1201 caccagcgac aaccaaattg atcatacaac atttgaacca acttttcatg tttaattttc
     1261 tatgtgtaac catatactgc atagaggacc atacatgtac cttttgcatg gtgcatacac
     1321 ctctaactta tattgtgaaa acaagtttag tttctatttg ctagtgttgt tacacaatca
     1381 aatttgttct tatcattctt tttttttgtg tgaaagttac attcttaaat aagtattatg
     1441 gaataagctt tcatattact taactttatt tcttactcgt tatttcaaaa ggtgaaaatt
     1501 acatttctac ccagacacac actaaataca gtacaaactc gaagcaaatg atttggattg
     1561 atttaaatca attttaggtt cacaagggca aggggtggaa tataaagaag aaagaaatgg
     1621 ataagggtgt catttggtgt gggcatatat ccaaccaaaa agggacattt tgcgggtatt
     1681 tgtgagtgtg tgagagagag agcgagcagt ttcagacaca gagagagggg tttgggattg
     1741 gagggattga cgctgcgtct cttcctcttc tctttcgatc catccctcat tcctaatcct
     1801 ccaatccaac cacctcagcc tcacactcac atatacacat gtctctctct atttcccttt
     1861 tcactttcac tttcatttct agggttccgt tctgatggct ctttcccttt cagatctgaa
     1921 cgcgtatcgt ccctcccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1981 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2041 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2101 ccgtgccctg gcccaccctc gtgaccacct tcacctacgg cgtgcagtgc ttcagccgct
     2161 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2221 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2281 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2341 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2401 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2461 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2521 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2581 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact cacggcatgg
     2641 acgagctgta caagtaaagc ggccgcccgg ctgcactgag gcctcagcag atcgttcaaa
     2701 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2761 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2821 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     2881 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     2941 tccgatgata agctgtcaaa catgagaatt agatctcaat tgaattcgta atcatggtca
     3001 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3061 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3121 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3181 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     5161 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
