LOCUS       40924_1139        5168 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM10-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5168)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1885
                     /label=GmScreamM10 promoter
                     /note="GmScreamM10 promoter"
                     /regulatory_class="promoter"
     CDS             1888..2604
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2640..2892
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2947..2963)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2971..2987)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2995..3025)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3040..3061)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3349..3937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4111..4968)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4969..5073)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttcagaac cgcactagtc
      421 ctctcaccac gagacaaaaa ccccaccagc tgcgctaaat tcgacaaagc ccgcgcccga
      481 attgcgccac tcacatcaga acaccgtttc acaagtgcct ccaagcacca cactccccac
      541 gcctcactcc cttcactctc cacgcccaac ggatccctca atgacgtcac cagattcaaa
      601 atgagatcaa ccgccaaaag ccgaagattc gacttcccct gtgccatctg cacaacatac
      661 ttcacaaacg caatttgatc atcaaattcc ataactttaa caacctccat gatggagtcc
      721 accgccaatg ctctaggctc ggccttctcc ggcgccttct tggccaaata cctcggaaaa
      781 ttaaccagcg ccttcttcac accatcgcat tggttactaa gaccggtgac aaatccaata
      841 gcaaatgtcc tcgccagcga cttcgccatg agaacaagcg aacacagcga tttcaacacc
      901 tcggcagcag tgttggaagg ttctccgtgc tcagatttga gaacttcctt cagcacgtgc
      961 gaacacaggc taagcaatct gctatacacc gccgcgttcc cgcacgtatc gagcgaggtc
     1021 acggggattt cagccacggt ttgaatcagg gacttgagag tctccgggaa gcggttcaag
     1081 tggatcaatc ccattacttt gactagcttc tccagcacgc ggaggagcac ccgggggtcg
     1141 tgttgggaac cggtgtccgg cgaatcgtcg tcgttttggg gattactcgc ccggccgcgg
     1201 cccggccgct tccgtttccg gttcatggag ttgtcttggg ccgggccggg gtgggagtgg
     1261 gccttgcagg agcggcggag ggagcggagg aaagaaagaa acgaaatggg tgtaaagagt
     1321 gtaaaaacgg gtgcgtttgg agcgaggagg agcgagagga agacgtcgga agcgagaaga
     1381 gagtggtgcg gtggtgaaga gtccattgct gaagcaatgg gaggaatgag agaagacgga
     1441 gagagattct tcgatggaag ctcatcgtag agagtctctg tgagagagtg tttgaggaga
     1501 aattgaagat ccattagggt ttgttcggag agtggtggcg gtggttgcgg gtttccacgg
     1561 agatcctcta gctcggagat tatgcgagac accgtttcct ccatttccgt catgctgtgt
     1621 gtgtccgagt cttcgtccac acactccacg ctctctattc aatttcaaat tcccgctttt
     1681 tcaattatgg gcctattatt ctatgggccg aaacagaagc ccaataggag aagggggttt
     1741 ttggtaattt gtgtgggtgt ggaaccagaa actgaaaacc ctggactata aaaaatagtt
     1801 gcacacagaa ttttcggtcc ctcttgctct cgcgtcgctg ctccgtacca gtttcactgc
     1861 ataaatttga gagaaaactc tgcaaccatg gtgagcaagg gcgaggagct gttcaccggg
     1921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg cgtgcagtgc
     2101 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2161 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2341 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2461 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2581 cacggcatgg acgagctgta caagtaaagc ggccgcccgg ctgcactgag gcctcagcag
     2641 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     2701 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     2761 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     2821 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     2881 tgttactaga tccgatgata agctgtcaaa catgagaatt agatctcaat tgaattcgta
     2941 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3001 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3061 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3121 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3781 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5101 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5161 ctttcgtc
//
