LOCUS       40924_1129        4920 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream9-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4920)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1637
                     /label=GmScream9 promoter
                     /note="GmScream9 promoter"
                     /regulatory_class="promoter"
     CDS             1640..2356
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2392..2644
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2699..2715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2723..2739)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2747..2777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2792..2813)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3101..3689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3863..4720)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4721..4825)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttacaatg ggcaccagaa
      421 taccctgact ttgtccattt cagatatgat ggaacatcat accaaattag actaagacag
      481 catcggcaaa aagtatactt tgctgaggga ctaaaccagt tcagaaaaga tttagcaatt
      541 tatgaatcta tcaccattca cttcttggtg caccagacct gcagaagacc acgaatgatc
      601 tcaagagagc accagacctg cagaagacca cgaatgatct caagagagca tatttggaca
      661 attgaaatca cccagtcgat gcttggtgca ccccaaccgc tggtaaccat caaaagttgt
      721 tctttgttcc ttatacaaat tcttgtttat atataagact attttttttt cattgcagag
      781 acttccaaat tgtgtcatta cacatgttaa tgcctgcggc caacacatga caatccttag
      841 aaaatttgga cctccactgc agtggaatgt tgtgctcctt gacattggaa ttggtcacag
      901 atatgttata caaccatggt atcaattcct tgccgacagt gatttctccc acgacgatga
      961 ggtttccttc tattacagac atgataaaat atggaaaatt atcatccgcc gccgcaggaa
     1021 agactgggat gacaatgaca gtgaataaat ttggaatgtt aaactgatac ttcttgataa
     1081 tagtgtttat ctttcttgct tttctgaaca atttatttac tatgtaaata tattatcaat
     1141 gtttaatcta ttttaatttg cacattaatt ttcattttat ttttacttta caaaacaaat
     1201 aaatatatat gcaaaaaaat ttacaaacga tgcacgggtt acaaactaat ttcattaaat
     1261 gctaatgcag attttgtgaa gtaaaactcc aattatgatg aaaaatacca ccaacaccac
     1321 ctgcgaaact gtatcccaac tgtccttaat aaaaatgtta aaaagtatat tattctcatt
     1381 tgtctgtcat aatttatgta ccccacttta atttttctga tgtactaaac cgagggcaaa
     1441 ctgaaacctg ttcctcatgc aaagccccta ctcaccatgt atcatgtacg tgtcatcacc
     1501 caacaactcc acttttgcta tataacaaca cccccgtcac actctccctc tctaacacac
     1561 accccactaa caattccttc acttgcagca ctgttgcatc atcatcttca ttgcaaaacc
     1621 ctaaacttca ccttcaagca tgctgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1681 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1741 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1801 gcccgtgccc tggcccaccc tcgtgaccac cttcacctac ggcgtgcagt gcttcagccg
     1861 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1921 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1981 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2041 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2101 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2161 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2221 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2281 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat
     2341 ggacgagctg tacaagtaaa gcggccgccc ggctgcactg aggcctcagc agatcgttca
     2401 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     2461 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     2521 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     2581 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     2641 gatccgatga taagctgtca aacatgagaa ttagatctca attgaattcg taatcatggt
     2701 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2761 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2821 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2881 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2941 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3001 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3061 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3121 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3181 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3241 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3301 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3361 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3421 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3481 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3541 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3601 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3661 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3721 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3781 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3841 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3901 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3961 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4021 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4081 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4141 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4201 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4261 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4321 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4381 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4441 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4501 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4561 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4621 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4681 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4741 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4801 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     4861 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
