LOCUS       40924_1094        5084 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream2-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5084)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      411..1801
                     /label=GmScream2 promoter
                     /note="GmScream2 promoter"
                     /regulatory_class="promoter"
     CDS             1804..2520
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2556..2808
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2863..2879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2887..2903)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2911..2941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2956..2977)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3265..3853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4027..4884)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4885..4989)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag tggttcaaca
      421 acacaagctt caagttttaa aaggaaaaat gtcagccaaa aactttaaat aaaatggtaa
      481 caaggaaatt attcaaaaat tacaaacctc gtcaaaatag gaaagaaaaa aagtttaggg
      541 atttagaaaa aacatcaatc tagttccacc ttattttata gagagaagaa actaatatat
      601 aagaactaaa aaacagaaga atagaaaaaa aaagtattga caggaaagaa aaagtagctg
      661 tatgcttata agtactttga ggatttgaat tctctcttat aaaacacaaa cacaattttt
      721 agattttatt taaataatca tcaatccgat tataattatt tatatatttt tctattttca
      781 aagaagtaaa tcatgagctt ttccaactca acatctattt tttttctctc aacctttttc
      841 acatcttaag tagtctcacc ctttatatat ataacttatt tcttaccttt tacattatgt
      901 aacttttatc accaaaacca acaactttaa aattttatta aatagactcc acaagtaact
      961 tgacactctt acattcatcg acattaactt ttatctgttt tataaatatt attgtgatat
     1021 aatttaatca aaataaccac aaactttcat aaaaggttct tattaagcat ggcatttaat
     1081 aagcaaaaac aactcaatca ctttcatata ggaggtagcc taagtacgta ctcaaaatgc
     1141 caacaaataa aaaaaaagtt gctttaataa tgccaaaaca aattaataaa acacttacaa
     1201 caccggattt tttttaatta aaatgtgcca tttaggataa atagttaata tttttaataa
     1261 ttatttaaaa agccgtatct actaaaatga tttttatttg gttgaaaata ttaatatgtt
     1321 taaatcaaca caatctatca aaattaaact aaaaaaaaaa taagtgtacg tggttaacat
     1381 tagtacagta atataagagg aaaatgagaa attaagaaat tgaaagcgag tctaattttt
     1441 aaattatgaa cctgcatata taaaaggaaa gaaagaatcc aggaagaaaa gaaatgaaac
     1501 catgcatggt cccctcgtca tcacgagttt ctgccatttg caatagaaac actgaaacac
     1561 ctttctcttt gtcacttaat tgagatgccg aagccacctc acaccatgaa cttcatgagg
     1621 tgtagcaccc aaggcttcca tagccatgca tactgaagaa tgtctcaagc tcagcaccct
     1681 acttctgtga cgtgtccctc attcaccttc ctctcttccc tataaataac cacgcctcag
     1741 gttctccgct tcacaactca aacattctct ccattggtcc ttaaacactc atcagtcatc
     1801 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1861 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1921 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1981 accctcgtga ccaccttcac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2041 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2101 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2161 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2221 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2281 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2341 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2401 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2461 gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga gctgtacaag
     2521 taaagcggcc gcccggctgc actgaggcct cagcagatcg ttcaaacatt tggcaataaa
     2581 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     2641 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     2701 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     2761 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg atgataagct
     2821 gtcaaacatg agaattagat ctcaattgaa ttcgtaatca tggtcatagc tgtttcctgt
     2881 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2941 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3001 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3061 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4021 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4081 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4141 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4201 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4261 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4321 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4381 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4441 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4501 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4561 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4621 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4681 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4741 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4801 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4861 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4921 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4981 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     5041 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
