LOCUS       62056_1096        7010 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             381..632
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     polyA_signal    2050..2184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2208..2224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2232..2248)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2256..2286)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2301..2322)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2610..3198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3372..4229)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4883..4899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5448..5827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5828..6031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          6167..6993
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
ORIGIN
        1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg
       61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc
      121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct
      181 agaccaccat gaaaaaggtt gaatccatca tcaggcccga aaagctggag atcgttaaga
      241 aggctctctc ggacgctgga tatgtgggta tgaccgtctc tgaagtcaaa ggctctggcg
      301 tccagggcgg catcttcgag aggtaccgag gaagggtgta ctctgcaggc tacaacagcg
      361 acaacgtcta catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      421 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga
      541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      601 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg
      661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      781 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      841 tgggctacgg tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct
      901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca
     1081 acggcaccat agtagatctg atccctaagg taaaaattga actagtggtg aaggaggagg
     1141 acgtcgataa cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg
     1201 gtaagatatt tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag
     1261 catctggtgg tggatccggt ggtggcggtg catctggtat gaaaaaggtt gaagctatta
     1321 ttcgcccaga gaaactggag attgttaaga aggccctgtc cgacgccggc tatgttggca
     1381 tgaccgtttc cgaggttaaa ggccgcggtg ccggcggtgg cgacctgatt cctaaggtga
     1441 agatcgagct ggttgttaag gaggaagacg ttgataatgt tatcgacatt atctgcgaga
     1501 acgcccgcac tggcaatcct ggtgacggca aaatcttcgt tataccggtc gagcgaattg
     1561 tgcgagtacg aaccaaagaa gagggtgcct ccggcggtgg cggtggctcc ggtggcgcca
     1621 gcggcatgaa gaaggttgag gccatcatcc gcccagagaa gctggaaatc gttaagaagg
     1681 ccttaaacga cgacggctac gtcggcatga cagttagcga ggttaaaggt cgcggtgccg
     1741 gtgggggtga tctgattcct aaggttaaaa tcgagctcgt tgttaaggag gaggacgttg
     1801 acaacattat cgatatcatc tgcgaaaacg ctcgcaccgg caacccaggc gacggcaaga
     1861 ttttcgtcat tcctgttgag agagtggtgc gtccccgtac gaaggaggag ggcaaggaag
     1921 cactgtgact cgacgaattc attgatcata atcagccata ccacatttgt agaggtttta
     1981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2161 aatgtatctt atcatgtctg gatccgtacc gagctcgcgt aatcatggtc atagctgttt
     2221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     2281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     2821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     3061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaataagc ggccgcggcc
     4261 atgccggcca ctagtctcga gttattattg aagcatttat cagggttatt gtctcatgag
     4321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4441 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4621 atcagagcag attgtactga gagtgcacca tatgaagacg tcgcctcctc actacttctg
     4681 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg
     4741 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4801 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     4861 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccatctgcag tgaataataa
     4921 aatgtgtgtt tgtccgaaat acgcgtttga gatttctgtc ccgactaaat tcatgtcgcg
     4981 cgatagtggt gtttatcgcc gatagagatg gcgatattgg aaaaatcgat atttgaaaat
     5041 atggcatatt gaaaatgtcg ccgatgtgag tttctgtgta actgatatcg ccatttttcc
     5101 aaaagttgat ttttgggcat acgcgatatc tggcgatacg cttatatcgt ttacggggga
     5161 tggcgataga cgcctttggt gacttgggcg attctgtgtg tcgcaaatat cgcagtttcg
     5221 atataggtga cagacgatat gaggctatat cgccgataga ggcgacatca agctggcaca
     5281 tggccaatgc atatcgatct atacattgaa tcaatattgg ccattagcca tattattcat
     5341 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atccatatca
     5401 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt
     5461 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     5521 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     5581 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     5641 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     5701 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     5761 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     5821 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     5881 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     5941 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     6001 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     6061 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     6121 ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta
     6181 tagagtctat aggcccaccc ccttggcttc ttatgcatgc tatactgttt ttggcttggg
     6241 gtctatacac ccccgcttcc tcatgttata ggtgatggta tagcttagcc tataggtgtg
     6301 ggttattgac cattattgac cactccccta ttggtgacga tactttccat tactaatcca
     6361 taacatggct ctttgccaca actctcttta ttggctatat gccaatacac tgtccttcag
     6421 agactgacac ggactctgta tttttacagg atggggtctc atttattatt tacaaattca
     6481 catatacaac accaccgtcc ccagtgcccg cagtttttat taaacataac gtgggatctc
     6541 cacgcgaatc tcgggtacgt gttccggaca tgggctcttc tccggtagcg gcggagcttc
     6601 tacatccgag ccctgctccc atgcctccag cgactcatgg tcgctcggca gctccttgct
     6661 cctaacagtg gaggccagac ttaggcacag cacgatgccc accaccacca gtgtgccgca
     6721 caaggccgtg gcggtagggt atgtgtctga aaatgagctc ggggagcggg cttgcaccgc
     6781 tgacgcattt ggaagactta aggcagcggc agaagaagat gcaggcagct gagttgttgt
     6841 gttctgataa gagtcagagg taactcccgt tgcggtgctg ttaacggtgg agggcagtgt
     6901 agtctgagca gtactcgttg ctgccgcgcg cgccaccaga cataatagct gacagactaa
     6961 cagactgttc ctttccatgg gtcttttctg cagtcaccgt ccttgacacg
//
