LOCUS       40924_800        6724 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector EXP4(+), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 6724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..30
                     /label=multipe cloning site
                     /note="multipe cloning site"
     regulatory      42..1022
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             1791..2582
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3475..3579
                     /label=AmpR promoter
     CDS             3580..4437
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4611..5199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      6416..6724
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctacta gtgaattctg atcactcgag gatctgctag aggtaccaca agtggtggtg
       61 gtggtatggg ttcatttggt ggtcgatccg atgaagaatc aagtgatcca aatgctatac
      121 ttggtctctt tgaagacgat attttctggg gtgataatga tgaatacttt agtgataact
      181 accgttatgt tgatgaagaa gatattgatg aagatttcaa tggtaatgat gaatttgaaa
      241 ttgatgttga aggtgatttc caagaagatg atttcgaaga agaatatgca ttttattaaa
      301 ttaaactttt tttcataaaa aaataataat aataatttaa taactaatca ttatcatcaa
      361 taataacatt ataatattac tttaataaca atactttttt ttaatattca aagactttac
      421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa acaaaccata aacaaaacta aaaataaatt
      481 aaaaaaaaaa ataaaaaaaa aaaaaaaaaa aaaaactaaa aatacaatta ttttattatt
      541 taaataaata attgtattat aatttgtatt tttgtgtttt tgtttttttt atttctttaa
      601 aatgatttat aacttttatt ttttataaag gaaaataaca ttttccttaa atgataaaag
      661 atcatttaaa acatttgtat taaaaggttc attaccatta tataaatcat taaataattg
      721 tttttgaact gtgaaaacga atttagtatg tttcaaatca gtgatttgtg gattatcaac
      781 taatattgat tgaattgttg gtgtatcatc atgagcataa gaaccatcat ccattatact
      841 ttcaacatga aaatattttg taattttttt attgattgat gaaatatatc taatgagttg
      901 atcagaattt aaacctaata ttgataattt tcttaacggt gcaattgatg aaatcattga
      961 accaaactct ttaacgaatg cattggataa ctttatcgat ctttgttttc tttgagagaa
     1021 ttagcggccg ctaattcaga ggtttatttt taaaaattac aaaaactaaa aagaaaaata
     1081 aaaaggaaaa atcttattaa tctgaaaatt acagatttgc cccaatttaa agaaaaatta
     1141 tccaaaataa tcaatcaaca acgatatctt tttgatagat atttataaaa actttatctt
     1201 ttttattttt tcaaagttgc gcaaaataat aaaattaaaa taaatataaa aactgtaaaa
     1261 agaaaaaaaa aagtgtaaag gtttattaat tatttaatta ttattcactt tttgtaatta
     1321 tttttttatt ttgaagaata atgatggata ttttatataa aaaaaaaaag agatatgaaa
     1381 aaataataat tataaaaaaa aaaaaaaaaa atagaatttt aaagttttag tacaaatgga
     1441 tgattttttt tttttttttt tttttttcct aaataattca agtaataaac aaagaattga
     1501 tattctgatg cctaattaaa aaagaaattt ttaaataaaa aatgggtttt ttttaagtaa
     1561 agttatttga aattgattga aattttcaaa ccatgggtgg tttttcgctt taaaattggg
     1621 attttatttt tattttttta tattttttat tttttatttt tttttttttt gaggtttctg
     1681 agattataaa atgaaatttt tttttttttt ttaattaatt caaaaaataa tcaaataaat
     1741 aaatataata taaaatggac ggagaagatg ttcaagctgg atcgtttcgc atgattgaac
     1801 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     1861 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     1921 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     1981 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     2041 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     2101 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     2161 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     2221 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     2281 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     2341 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     2401 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     2461 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2521 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     2581 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     2641 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     2701 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg
     2761 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt
     2821 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca
     2881 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcccgg atcagcttta
     2941 tctttttgat tttcatactc tttggctgtt ttacatctaa tgcatatatt tatcttcacc
     3001 attcaaaatg tatctgtaaa taataaattt tttaaatata tttgattttt ttgatagaaa
     3061 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aattaaaata acttaataat tattaagatt
     3121 taaaaaaaaa aaattaaaaa aaaaagaaaa tattagatta ttacaacaat ttgttttttt
     3181 attatttatt aaataattta gaaacatttt ctgtgaacaa ttgatggacc ctcaggaaga
     3241 tcgcactcca gccagctttc cggcaccgct tctggtgccg gaaaccaggc aaagcgccat
     3301 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tggcggggat cagcggccgc
     3361 tatcagatca attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3421 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3781 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     3841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     4141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4741 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgctgact tccgcgtttc
     5401 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     5461 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     5521 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     5581 tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
     5641 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
     5701 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     5761 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     5821 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg
     5881 acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc
     5941 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     6001 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
     6061 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg
     6121 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     6181 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     6241 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     6301 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     6361 ctcaagggca tcggtcgagc agctgaagct tgcatgcctg caggtcgact ctagataaaa
     6421 aaaattttta tttattttta tttattttga attaaataga ttacaaatta attaatccca
     6481 tcaaatcttt aaaaaaaaaa atggtttaaa aaaacttggg ttggttaatt attatttgaa
     6541 aattttaaaa cccaaattaa aaaaaaaaaa tgggattcaa aaattttttt tttttttttt
     6601 tttttttttt tttttttttt tttttttttt tttttttttt tcagattgca taaaaagatt
     6661 tttttttttt tttttttctt atttcttaaa acaaataaat taaattaaat aaaaaataaa
     6721 aatc
//
